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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
EYFP-Abr in pCCL-cppt178-MNDU3
 
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RRID:Addgene_38155 Abr Homo sapiens Ampicillin PMID:17116687 Constructed by Jess Cunnick. Abr was first subcloned into pEYFP-C1 Bgl II x KpnI as a 0.4 kb 5' BamHI/BstEII + 2.2 kb BstEII- KpnI fragment. The EYFP-Abr fusion was isolated as a 5' AgeI- 3' KpnI fragment and inserted into pCCL-cppt178-MNDU3-X3 digested with Xma I x Kpn I. Backbone Marker:Don Kohn [email protected]; Backbone Size:6600; Vector Backbone:pCCL-cppt178-MNDU3; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:14:19 1
Abr-pLEF
 
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RRID:Addgene_38157 ABR Homo sapiens Ampicillin PMID:18070886 Constructed by Suparna Mishra. Generated in a 3-way ligation consisting of 5' 0.4 kb BamHI/BstEII + 3' 2.2 kb BstEII/EcoRI ABR fragments into pLEF digested with BamHIxEcoRI. The ABR insert contains the natural TAG and 3' UT sequences. Addgene sequencing found an E24G point mutation--this does not alter plasmid function. Backbone Size:7400; Vector Backbone:pLEF; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:14:19 0
GST-Bcr pLEF
 
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RRID:Addgene_38160 Bcr Homo sapiens Ampicillin PMID:14614449 Constructed by Anja Reichert. Generated in a 3-way ligation consisting of 5' 0.6 kb BamHI/SalI + 3' 3.5 kb SalI/XbaI BCR fragments into pLEF digested with BamHIxXbaI. The BCR insert contains the natural TAG and 3' UT sequences. At the 3' end there are polylinker sequences from pSK. Backbone Size:7400; Vector Backbone:pLEF; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:14:19 0
Bcr/Abl P210 in pAcG2T
 
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RRID:Addgene_38161 Bcr/Abl P210 Homo sapiens Ampicillin Constructed by Nora Heisterkamp. Made using a 3-way ligation with 0.6 kb 5' BamHI-SalI from BCR + 3' 7.5 kb Sal I-EcoRI into pAcG2T. Not evaluated for protein production. Backbone Marker:Pharmingen; Backbone Size:8530; Vector Backbone:pAcG2T; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:14:19 0
pPK719
 
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RRID:Addgene_38149 cbr-unc-119::FRT*::galk::FRT* Caenorhabditis elegans Ampicillin This plasmid carries a complementary cassette consisting of a genomic copy of the C. briggsae unc-119 gene and the selectable galK marker flanked by FRT* sites in the presumptive 3’ UTR of the Cbr-unc-119 gene. The 3.6 kb Cbr-unc-119::FRT*::galk::FRT* cassette can be targeted by recombineering to insert between nts 281:282 in the pCC1FOS fosmid vector backbone. In other words, this cassette can be readily inserted in all fosmids contained in the C. elegans library developed by Don Moerman and colleagues. We have tested the construct in fosmid recombineering and have shown that it rescues unc-119(ed3) mutants. Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript KSII(+); Vector Types:Worm Expression, recombineering; Bacterial Resistance:Ampicillin 2026-08-15 01:14:19 1
pPK348
 
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RRID:Addgene_38145 ptc-3::GFP Caenorhabditis elegans Ampicillin PMID:21215260 The pPK348 plasmid was derived by inserting the GFP cassette from pPD103.87 (Addgene plasmid 1524) flanked by XbaI ends into the unique SpeI site present in exon 8 of ptc-3 and carries the entire predicted ptc-3 locus comprised of a 6.9 kb genomic coding region fused in-frame to GFP and more than 5 kb of 5′ upstream sequence, including the putative promoter Please note that Addgene's sequencing results identified several mismatches in the region of bp#722-824 when compared to the full plasmid sequence provided by the depositing laboratory. The depositor states that these mismatches are not a concern for the function of the plasmid. Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript KSII(+); Vector Types:Worm Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:14:19 0
pPK700
 
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RRID:Addgene_38146 let-60p::ptc-3::gfp Caenorhabditis elegans Ampicillin PMID:21215260 The pPK700 plasmid was derived by ligating the let-60 promoter region to ptc-3::gfp expression cassette, which was PCR amplified from pPK348 (Addgene plasmid 38145) Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript KSII(+); Vector Types:Worm Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:14:19 0
RCIscript-GoldyTALEN
 
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10+ mentions
RRID:Addgene_38142 GoldyTALEN Synthetic Ampicillin PMID:23027955 Please note that this plasmid can be prone to recombination in bacteria. We recommend storing and propagating the construct in Stbl3 cells and screen 4-6 colonies by sequencing with TAL_F1 (ttggcgtcggcaaacagtgg) primer. The F1 origin feature in the full plasmid sequence is in reverse compliment orientation. Please view Addgene's quality control sequence for the most accurate information. The flipped orientation does not affect the SacI site necessary to linearize the plasmid. For further reference, this plasmid was also used in the Bedell et al Nature 2012 publication (PMID 23000899). For more information on Carlson TALEN Add-On Plasmids please refer to: http://www.addgene.org/TALeffector/goldengate/add-ons/#carlson Backbone Size:2900; Vector Backbone:pBSK; Vector Types:mRNA transcription vector; Bacterial Resistance:Ampicillin Truncate at N and C terminous 2026-08-15 01:14:19 18
pC-GoldyTALEN
 
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RRID:Addgene_38143 GoldyTALEN Synthetic Ampicillin PMID:23027955 For further reference, this plasmid was also used in the Bedell et al Nature 2012 publication (PMID 23000899). For more information on Carlson TALEN Add-On Plasmids please refer to: http://www.addgene.org/TALeffector/goldengate/add-ons/#carlson Backbone Size:4000; Vector Backbone:pBSK; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Truncate at N and C terminous 2026-08-15 01:14:19 12
p1177-X1-8
 
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RRID:Addgene_38150 Carboxypeptidase A M. anisopliae Ampicillin PMID:21073956 Backbone Marker:Invitrogen; Backbone Size:6526; Vector Backbone:pFastBac-1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:14:19 0
pcDNA4-FLAG-Jhdm2a
 
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RRID:Addgene_38136 Jhdm2a Mus musculus Ampicillin PMID:22722204 Backbone Size:5100; Vector Backbone:pcDNA4/myc-His A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin protein starts at aa115 2026-08-15 01:14:19 3
pCW57.1-4EBP1_4xAla
 
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10+ mentions
RRID:Addgene_38240 4EBP1 Rattus norvegicus Ampicillin PMID:22552098 Backbone Marker:Broad Insitute; Backbone Size:5000; Vector Backbone:pCW57.1; Vector Types:Mammalian Expression, Lentiviral, Doxycycline inducible; Bacterial Resistance:Ampicillin T37A, T46A, S65A, T70A (dominant negative) 2026-08-15 01:14:20 15
pIS1-Vim/Actb5UTR-renilla
 
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RRID:Addgene_38238 Vim promoter with Actb 5UTR/Renilla Mus musculus Ampicillin PMID:22552098 This vector originally contained the mouse Actb 5' UTR followed by the renilla luciferase ORF. In the current version, the promoter and first 30 nt of the 5' UTR have been replaced by the mouse Vim promoter and first 30 nt of the Vim 5' UTR, respectively. Backbone Size:4000; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin Actb promoter (1000 nt upstream) and 5' UTR was replaced with the Vim promoter and first 30 nt of Actb 5' UTR 2026-08-15 01:14:20 0
pEBB HA cIAP1 H588A
 
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1+ mentions
RRID:Addgene_38233 cIAP1 H588A Homo sapiens Ampicillin PMID:19243308 Backbone Marker:Baltimore Lab; Backbone Size:5350; Vector Backbone:pEBB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin H588A 2026-08-15 01:14:20 1
pMXs-IP-EGFP-LC3
 
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1+ mentions
RRID:Addgene_38195 microtubule-associated protein 1 light chain 3 beta Rattus norvegicus Ampicillin PMID:18443221 Backbone Marker:Dr. Toshio Kitamura of the University of Tokyo ; Backbone Size:5847; Vector Backbone:pMXs-IP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-08-15 01:14:20 2
pMXs-IP-EGFP-ULK1(K46N)
 
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RRID:Addgene_38197 a novel protein kinase structurally related to C. elegans UNC-51 Mus musculus Ampicillin PMID:18443221 Backbone Marker:Dr. Toshio Kitamura of the University of Tokyo ; Backbone Size:5847; Vector Backbone:pMXs-IP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin changed Lysine 46 to Asparagine 2026-08-15 01:14:20 2
pMXs-IP-EGFP-ULK1(1-828)
 
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RRID:Addgene_38198 a novel protein kinase structurally related to C. elegans UNC-51 Mus musculus Ampicillin PMID:18443221 Backbone Marker:Dr. Toshio Kitamura of the University of Tokyo ; Backbone Size:5847; Vector Backbone:pMXs-IP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin deleted amino acids 829-1051 2026-08-15 01:14:20 0
pTH736-CEN-staHIS3
 
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RRID:Addgene_38228 HIS3 Saccharomyces cerevisiae Ampicillin PMID:24357599 Backbone Size:6614; Vector Backbone:pCENBEVY-U; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:14:20 0
HA-tagged Bcr
 
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RRID:Addgene_38189 BCR Homo sapiens Ampicillin Constructed by Young Jin Cho. A human BCR cDNA was inserted into pKH3 (Addgene plasmid #12555; obtained from Martin Schwartz) digested with BamHI x EcoRI in a three-way ligation (1.7 kb BamHI-SstI + 2.6 kb SstI-EcoRI). The 1.7 kb BamHI-SstI fragment was isolated from Bcr in pAcG2T (AddGene plasmid #38163). The 5’ sequence is GGA TCC CCC GGG CTG CAG GAA TTC GAT GGC CGC GCC ATG-start BCR. Backbone Size:4805; Vector Backbone:pKH3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:14:20 2
pTH743-CEN-RLuc/slowstaCFLuc
 
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RRID:Addgene_38223 Firefly Luciferase Photinus pyralis Ampicillin PMID:24357599 Backbone Size:7122; Vector Backbone:pCENBEVY-U; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin The last three codons of the full-length Firefly luciferase gene have been deleted to yield a luciferase variant which is fully functional, but no longer localises to peroxisomes. 2026-08-15 01:14:20 0

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