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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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EYFP-Abr in pCCL-cppt178-MNDU3 Resource Report Resource Website 1+ mentions |
RRID:Addgene_38155 | Abr | Homo sapiens | Ampicillin | PMID:17116687 | Constructed by Jess Cunnick. Abr was first subcloned into pEYFP-C1 Bgl II x KpnI as a 0.4 kb 5' BamHI/BstEII + 2.2 kb BstEII- KpnI fragment. The EYFP-Abr fusion was isolated as a 5' AgeI- 3' KpnI fragment and inserted into pCCL-cppt178-MNDU3-X3 digested with Xma I x Kpn I. | Backbone Marker:Don Kohn [email protected]; Backbone Size:6600; Vector Backbone:pCCL-cppt178-MNDU3; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:19 | 1 | |
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Abr-pLEF Resource Report Resource Website |
RRID:Addgene_38157 | ABR | Homo sapiens | Ampicillin | PMID:18070886 | Constructed by Suparna Mishra. Generated in a 3-way ligation consisting of 5' 0.4 kb BamHI/BstEII + 3' 2.2 kb BstEII/EcoRI ABR fragments into pLEF digested with BamHIxEcoRI. The ABR insert contains the natural TAG and 3' UT sequences. Addgene sequencing found an E24G point mutation--this does not alter plasmid function. | Backbone Size:7400; Vector Backbone:pLEF; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:19 | 0 | |
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GST-Bcr pLEF Resource Report Resource Website |
RRID:Addgene_38160 | Bcr | Homo sapiens | Ampicillin | PMID:14614449 | Constructed by Anja Reichert. Generated in a 3-way ligation consisting of 5' 0.6 kb BamHI/SalI + 3' 3.5 kb SalI/XbaI BCR fragments into pLEF digested with BamHIxXbaI. The BCR insert contains the natural TAG and 3' UT sequences. At the 3' end there are polylinker sequences from pSK. | Backbone Size:7400; Vector Backbone:pLEF; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:19 | 0 | |
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Bcr/Abl P210 in pAcG2T Resource Report Resource Website |
RRID:Addgene_38161 | Bcr/Abl P210 | Homo sapiens | Ampicillin | Constructed by Nora Heisterkamp. Made using a 3-way ligation with 0.6 kb 5' BamHI-SalI from BCR + 3' 7.5 kb Sal I-EcoRI into pAcG2T. Not evaluated for protein production. | Backbone Marker:Pharmingen; Backbone Size:8530; Vector Backbone:pAcG2T; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:19 | 0 | ||
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pPK719 Resource Report Resource Website 1+ mentions |
RRID:Addgene_38149 | cbr-unc-119::FRT*::galk::FRT* | Caenorhabditis elegans | Ampicillin | This plasmid carries a complementary cassette consisting of a genomic copy of the C. briggsae unc-119 gene and the selectable galK marker flanked by FRT* sites in the presumptive 3’ UTR of the Cbr-unc-119 gene. The 3.6 kb Cbr-unc-119::FRT*::galk::FRT* cassette can be targeted by recombineering to insert between nts 281:282 in the pCC1FOS fosmid vector backbone. In other words, this cassette can be readily inserted in all fosmids contained in the C. elegans library developed by Don Moerman and colleagues. We have tested the construct in fosmid recombineering and have shown that it rescues unc-119(ed3) mutants. | Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript KSII(+); Vector Types:Worm Expression, recombineering; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:19 | 1 | ||
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pPK348 Resource Report Resource Website |
RRID:Addgene_38145 | ptc-3::GFP | Caenorhabditis elegans | Ampicillin | PMID:21215260 | The pPK348 plasmid was derived by inserting the GFP cassette from pPD103.87 (Addgene plasmid 1524) flanked by XbaI ends into the unique SpeI site present in exon 8 of ptc-3 and carries the entire predicted ptc-3 locus comprised of a 6.9 kb genomic coding region fused in-frame to GFP and more than 5 kb of 5′ upstream sequence, including the putative promoter Please note that Addgene's sequencing results identified several mismatches in the region of bp#722-824 when compared to the full plasmid sequence provided by the depositing laboratory. The depositor states that these mismatches are not a concern for the function of the plasmid. | Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript KSII(+); Vector Types:Worm Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:19 | 0 | |
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pPK700 Resource Report Resource Website |
RRID:Addgene_38146 | let-60p::ptc-3::gfp | Caenorhabditis elegans | Ampicillin | PMID:21215260 | The pPK700 plasmid was derived by ligating the let-60 promoter region to ptc-3::gfp expression cassette, which was PCR amplified from pPK348 (Addgene plasmid 38145) | Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript KSII(+); Vector Types:Worm Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:19 | 0 | |
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RCIscript-GoldyTALEN Resource Report Resource Website 10+ mentions |
RRID:Addgene_38142 | GoldyTALEN | Synthetic | Ampicillin | PMID:23027955 | Please note that this plasmid can be prone to recombination in bacteria. We recommend storing and propagating the construct in Stbl3 cells and screen 4-6 colonies by sequencing with TAL_F1 (ttggcgtcggcaaacagtgg) primer. The F1 origin feature in the full plasmid sequence is in reverse compliment orientation. Please view Addgene's quality control sequence for the most accurate information. The flipped orientation does not affect the SacI site necessary to linearize the plasmid. For further reference, this plasmid was also used in the Bedell et al Nature 2012 publication (PMID 23000899). For more information on Carlson TALEN Add-On Plasmids please refer to: http://www.addgene.org/TALeffector/goldengate/add-ons/#carlson | Backbone Size:2900; Vector Backbone:pBSK; Vector Types:mRNA transcription vector; Bacterial Resistance:Ampicillin | Truncate at N and C terminous | 2026-08-15 01:14:19 | 18 |
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pC-GoldyTALEN Resource Report Resource Website 10+ mentions |
RRID:Addgene_38143 | GoldyTALEN | Synthetic | Ampicillin | PMID:23027955 | For further reference, this plasmid was also used in the Bedell et al Nature 2012 publication (PMID 23000899). For more information on Carlson TALEN Add-On Plasmids please refer to: http://www.addgene.org/TALeffector/goldengate/add-ons/#carlson | Backbone Size:4000; Vector Backbone:pBSK; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Truncate at N and C terminous | 2026-08-15 01:14:19 | 12 |
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p1177-X1-8 Resource Report Resource Website |
RRID:Addgene_38150 | Carboxypeptidase A | M. anisopliae | Ampicillin | PMID:21073956 | Backbone Marker:Invitrogen; Backbone Size:6526; Vector Backbone:pFastBac-1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:19 | 0 | ||
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pcDNA4-FLAG-Jhdm2a Resource Report Resource Website 1+ mentions |
RRID:Addgene_38136 | Jhdm2a | Mus musculus | Ampicillin | PMID:22722204 | Backbone Size:5100; Vector Backbone:pcDNA4/myc-His A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | protein starts at aa115 | 2026-08-15 01:14:19 | 3 | |
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pCW57.1-4EBP1_4xAla Resource Report Resource Website 10+ mentions |
RRID:Addgene_38240 | 4EBP1 | Rattus norvegicus | Ampicillin | PMID:22552098 | Backbone Marker:Broad Insitute; Backbone Size:5000; Vector Backbone:pCW57.1; Vector Types:Mammalian Expression, Lentiviral, Doxycycline inducible; Bacterial Resistance:Ampicillin | T37A, T46A, S65A, T70A (dominant negative) | 2026-08-15 01:14:20 | 15 | |
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pIS1-Vim/Actb5UTR-renilla Resource Report Resource Website |
RRID:Addgene_38238 | Vim promoter with Actb 5UTR/Renilla | Mus musculus | Ampicillin | PMID:22552098 | This vector originally contained the mouse Actb 5' UTR followed by the renilla luciferase ORF. In the current version, the promoter and first 30 nt of the 5' UTR have been replaced by the mouse Vim promoter and first 30 nt of the Vim 5' UTR, respectively. | Backbone Size:4000; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin | Actb promoter (1000 nt upstream) and 5' UTR was replaced with the Vim promoter and first 30 nt of Actb 5' UTR | 2026-08-15 01:14:20 | 0 |
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pEBB HA cIAP1 H588A Resource Report Resource Website 1+ mentions |
RRID:Addgene_38233 | cIAP1 H588A | Homo sapiens | Ampicillin | PMID:19243308 | Backbone Marker:Baltimore Lab; Backbone Size:5350; Vector Backbone:pEBB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | H588A | 2026-08-15 01:14:20 | 1 | |
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pMXs-IP-EGFP-LC3 Resource Report Resource Website 1+ mentions |
RRID:Addgene_38195 | microtubule-associated protein 1 light chain 3 beta | Rattus norvegicus | Ampicillin | PMID:18443221 | Backbone Marker:Dr. Toshio Kitamura of the University of Tokyo ; Backbone Size:5847; Vector Backbone:pMXs-IP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:20 | 2 | ||
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pMXs-IP-EGFP-ULK1(K46N) Resource Report Resource Website 1+ mentions |
RRID:Addgene_38197 | a novel protein kinase structurally related to C. elegans UNC-51 | Mus musculus | Ampicillin | PMID:18443221 | Backbone Marker:Dr. Toshio Kitamura of the University of Tokyo ; Backbone Size:5847; Vector Backbone:pMXs-IP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | changed Lysine 46 to Asparagine | 2026-08-15 01:14:20 | 2 | |
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pMXs-IP-EGFP-ULK1(1-828) Resource Report Resource Website |
RRID:Addgene_38198 | a novel protein kinase structurally related to C. elegans UNC-51 | Mus musculus | Ampicillin | PMID:18443221 | Backbone Marker:Dr. Toshio Kitamura of the University of Tokyo ; Backbone Size:5847; Vector Backbone:pMXs-IP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | deleted amino acids 829-1051 | 2026-08-15 01:14:20 | 0 | |
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pTH736-CEN-staHIS3 Resource Report Resource Website |
RRID:Addgene_38228 | HIS3 | Saccharomyces cerevisiae | Ampicillin | PMID:24357599 | Backbone Size:6614; Vector Backbone:pCENBEVY-U; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:20 | 0 | ||
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HA-tagged Bcr Resource Report Resource Website 1+ mentions |
RRID:Addgene_38189 | BCR | Homo sapiens | Ampicillin | Constructed by Young Jin Cho. A human BCR cDNA was inserted into pKH3 (Addgene plasmid #12555; obtained from Martin Schwartz) digested with BamHI x EcoRI in a three-way ligation (1.7 kb BamHI-SstI + 2.6 kb SstI-EcoRI). The 1.7 kb BamHI-SstI fragment was isolated from Bcr in pAcG2T (AddGene plasmid #38163). The 5’ sequence is GGA TCC CCC GGG CTG CAG GAA TTC GAT GGC CGC GCC ATG-start BCR. | Backbone Size:4805; Vector Backbone:pKH3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:20 | 2 | ||
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pTH743-CEN-RLuc/slowstaCFLuc Resource Report Resource Website |
RRID:Addgene_38223 | Firefly Luciferase | Photinus pyralis | Ampicillin | PMID:24357599 | Backbone Size:7122; Vector Backbone:pCENBEVY-U; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | The last three codons of the full-length Firefly luciferase gene have been deleted to yield a luciferase variant which is fully functional, but no longer localises to peroxisomes. | 2026-08-15 01:14:20 | 0 |
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