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Species: Saccharomyces cerevisiae
Genetic Insert: 6his-Cdc12 + Cdc11
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET-Duet; Vector Types:Bacterial Expression, bicistronic; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23889817
Proper citation: RRID:Addgene_131162 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cdc3 + Cdc10
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pACYC-Duet1; Vector Types:Bacterial Expression, bicistronic; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23889817
Comments: combine with pET-Cdc12-Cdc11 for expression of septin rods
Proper citation: RRID:Addgene_131160 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsp104 L553W
Vector Backbone Description: Backbone Size:5586; Vector Backbone:pGAL; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11983167
Proper citation: RRID:Addgene_1313 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsp104 L892W
Vector Backbone Description: Backbone Size:5586; Vector Backbone:pGAL; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11983167
Proper citation: RRID:Addgene_1315 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsp104 (773-908)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4746; Vector Backbone:pPROEX-HTb; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11983167
Proper citation: RRID:Addgene_1316 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MalE-6xHis-Ulp1
Vector Backbone Description: Vector Backbone:pTST101 ; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19127343
Proper citation: RRID:Addgene_135086 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SPT23
Vector Backbone Description: Backbone Size:0; Vector Backbone:pPS808; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11598205
Comments: Expresses both the unprocessed and processed forms of Spt23. pPS808 (or pCGF-1a) is YEp352-derived vector with GAL1 promoter (EcoRI/XbaI fragment), GFP (BglII/BamHI fragment), and 2 micron ori.
Proper citation: RRID:Addgene_1351 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MGA2
Vector Backbone Description: Backbone Size:0; Vector Backbone:pPS808; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11598205
Comments: Expresses both the unprocessed and processed forms of MGA2. pPS808 (or pCGF-1a) is YEp352-derived vector with GAL1 promoter (EcoRI/XbaI fragment), GFP (BglII/BamHI fragment), and 2 micron ori.
Proper citation: RRID:Addgene_1352 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PSE1
Vector Backbone Description: Backbone Size:7300; Vector Backbone:pPS934; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9238021
Comments: PSE1 replaces NUF2 of pPS934, CEN plasmid.
Proper citation: RRID:Addgene_1354 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SpunH2Bpr hph-tdTomato ADH1t
Vector Backbone Description: Backbone Size:9576; Vector Backbone:pGI3; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32392127
Comments: The pGI3 vector was a gift from Giuseppe Ianiri
Proper citation: RRID:Addgene_135484 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: hph SpunH2A/Bpr H2B-tdTomato
Vector Backbone Description: Backbone Size:9576; Vector Backbone:pGI3EM11; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32392127
Comments: The pGI3 vector was a gift from Giuseppe Ianiri
Proper citation: RRID:Addgene_135487 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: hph SpunH2A/Bpr LifeAct-tdTomato
Vector Backbone Description: Backbone Size:12668; Vector Backbone:pGI3EM20C; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32392127
Comments: The pGI3 vector was a gift from Giuseppe Ianiri
Proper citation: RRID:Addgene_135488 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: hph SpunH2A/Bpr hph-mCerulean3
Vector Backbone Description: Backbone Size:10906; Vector Backbone:pGI3EM18; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32392127
Comments: mCerulean3 was obtained by PCR of mCerulean3-N1 (Addgene plasmid \# 54730), a plasmid created by the Davidson lab
The pGI3 vector was a gift from Giuseppe Ianiri
Proper citation: RRID:Addgene_135491 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sup35NM
Vector Backbone Description: Backbone Marker:Gregory Newby; Backbone Size:8600; Vector Backbone:pGAN147; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29056345
Comments: This is the basic yTRAP sensor to sense aggregation of Sup35 or [PSI+]. It should be linearized with NotI-HF enzyme prior to integration into the yeast genome. The sensor integrates into the HO locus, and can be selected for using nourseothricin.
Proper citation: RRID:Addgene_129203 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RNQ1
Vector Backbone Description: Backbone Marker:Gregory Newby; Backbone Size:8600; Vector Backbone:pGAN147; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29056345
Comments: This is the basic yTRAP sensor to sense aggregation of Rnq1 or [RNQ+]. It should be linearized with NotI-HF enzyme prior to integration into the yeast genome. The sensor integrates into the HO locus, and can be selected for using nourseothricin.
Proper citation: RRID:Addgene_129204 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FlpO-2A-Cre
Vector Backbone Description: Backbone Marker:ThermoFisher; Vector Backbone:pOG44; Vector Types:Mammalian Expression, Cre/Lox, Unspecified, Episomal expression vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31539496
Proper citation: RRID:Addgene_129419 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 15xUAS-∆pes-10-Flp-SL2
Vector Backbone Description: Vector Backbone:pBN412; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32550473
Comments: 15xUAS sequence from pHW394 inserted into pBN412
Proper citation: RRID:Addgene_129555 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ADP-ribosylation factor GTPase-activating protein
Vector Backbone Description: Vector Backbone:PQE; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31331965
Proper citation: RRID:Addgene_129484 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Glo3 ΔGRM ΔC
Vector Backbone Description: Vector Backbone:P415 Met25; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31331965
Proper citation: RRID:Addgene_129487 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: snR6
Vector Backbone Description: Vector Backbone:pSE358; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31558568
Proper citation: RRID:Addgene_135591 Copy
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