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Species: Other
Genetic Insert: sfGFP Dropout Cassette
Vector Backbone Description: Vector Backbone: pNW33N; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_235362 Copy
Species: Other
Genetic Insert: Bba_B0015
Vector Backbone Description: Backbone Marker:iGEM; Vector Backbone:pSB1A2; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_235369 Copy
Species: Other
Genetic Insert: S718 term
Vector Backbone Description: Backbone Marker:iGEM; Vector Backbone:pSB1A2; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_235439 Copy
Species: Other
Genetic Insert: sfGFP Dropout Cassette
Vector Backbone Description: Vector Backbone:pS797; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: To enable the conjugal transformation of Geobacillus species. plasmid pS797 was engineered to include an origin of transfer (ORI T) that incorporates the Nic region and traJ gene from the RP4 conjugal plasmid. Additionally, it features two replication origins-ColE for E. coli and BST1 for Geobacillus-to support propagation in both hosts. The plasmid also carries two antibiotic resistance markers, which allow for selection with ampicillin in E. coli and kanamycin in Geobacillus.
Proper citation: RRID:Addgene_235443 Copy
Species: Other
Genetic Insert: lacZ Dropout Cassette
Vector Backbone Description: Vector Backbone:pS797; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: To enable the conjugal transformation of Geobacillus species. plasmid pS797 was engineered to include an origin of transfer (ORI T) that incorporates the Nic region and traJ gene from the RP4 conjugal plasmid. Additionally, it features two replication origins-ColE for E. coli and BST1 for Geobacillus-to support propagation in both hosts. The plasmid also carries two antibiotic resistance markers, which allow for selection with ampicillin in E. coli and kanamycin in Geobacillus.
Proper citation: RRID:Addgene_235445 Copy
Species: Other
Genetic Insert: miRFP703
Vector Backbone Description: Backbone Size:9591; Vector Backbone:pHR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_216667 Copy
Species: Other
Genetic Insert: BFP
Vector Backbone Description: Backbone Marker:Addgene #79119; Backbone Size:8689; Vector Backbone:pHR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_216665 Copy
Species: Other
Genetic Insert: TurboID-FKBP
Vector Backbone Description: Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39271111
Proper citation: RRID:Addgene_228019 Copy
Species: Other
Genetic Insert: TurboID-FKBP
Vector Backbone Description: Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39271111
Proper citation: RRID:Addgene_228014 Copy
Species: Other
Genetic Insert: TurboID-FRB
Vector Backbone Description: Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39271111
Proper citation: RRID:Addgene_228016 Copy
Species: Other
Genetic Insert: TurboID-FKBP
Vector Backbone Description: Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39271111
Proper citation: RRID:Addgene_228007 Copy
Species: Other
Genetic Insert: TurboID-FRB
Vector Backbone Description: Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39271111
Proper citation: RRID:Addgene_227997 Copy
Species: Other
Genetic Insert: TurboID-FKBP
Vector Backbone Description: Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39271111
Proper citation: RRID:Addgene_227998 Copy
Species: Other
Genetic Insert: CBS4-miniDFR::RUBY::OCS
Vector Backbone Description: Vector Backbone:pICH47751; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39081031
Comments: Please visit https://doi.org/10.1101/2024.03.23.586378 for bioRxiv preprint.
Proper citation: RRID:Addgene_222450 Copy
Species: Other
Genetic Insert: CBS4-miniDFR::Cre::NOS
Vector Backbone Description: Vector Backbone:pICH47791; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39081031
Comments: Please visit https://doi.org/10.1101/2024.03.23.586378 for bioRxiv preprint.
Proper citation: RRID:Addgene_222452 Copy
Species: Other
Genetic Insert: 35S::CUP2-p65::NOS
Vector Backbone Description: Vector Backbone:pICH47742; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39081031
Comments: Please visit https://doi.org/10.1101/2024.03.23.586378 for bioRxiv preprint.
Proper citation: RRID:Addgene_222454 Copy
Species: Other
Genetic Insert: Mas::CUP2-VP16::NOS
Vector Backbone Description: Vector Backbone:pICH47742; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39081031
Comments: Please visit https://doi.org/10.1101/2024.03.23.586378 for bioRxiv preprint.
Proper citation: RRID:Addgene_222455 Copy
Species: Other
Genetic Insert: miniTurboID-FRB
Vector Backbone Description: Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39271111
Proper citation: RRID:Addgene_228000 Copy
Species: Other
Genetic Insert: TurboID-FRB
Vector Backbone Description: Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39271111
Proper citation: RRID:Addgene_228004 Copy
Species: Other
Genetic Insert: BASU-FRB
Vector Backbone Description: Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39271111
Proper citation: RRID:Addgene_227992 Copy
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