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Species: Mus musculus
Genetic Insert: Dicer shRNA
Vector Backbone Description: Backbone Size:7700; Vector Backbone:pSicoR PGK puro; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17401365
Comments: shRNA sequence: TGCATGGTGG TGTCGATATT TTCAAGAGAA ATATCGACAC CACCATGCTT TTTTC
Proper citation: RRID:Addgene_14781 Copy
Species: Mus musculus
Genetic Insert: Hmga2 truncated
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17322030
Comments: The 3' UTR of the mouse Hmga2 cDNA (BC052158) was subcloned into pCR2.1-TOPO (Invitrogen) for site-directed mutagenesis. To disrupt each let-7 complementary site, the nucleotides that paired to nucleotides 3 and 5 of the miRNA were substituted (see Author's map), using the Quikchange site-directed mutagenesis kit (Stratagene). The UTR fragment was then cloned back into the vector containing the Hmga2 cDNA (pCMV•SPORT6.1). To construct the mammalian expression vectors, Hmga2 inserts were excised from the pCMV•SPORT6.1 constructs using EcoRI restriction sites and cloned into pcDNA3.1 (Invitrogen).
Proper citation: RRID:Addgene_14793 Copy
Species: Mus musculus
Genetic Insert: Hmga2 m7
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17322030
Comments: The 3' UTR of the mouse Hmga2 cDNA (BC052158) was subcloned into pCR2.1-TOPO (Invitrogen) for site-directed mutagenesis. To disrupt each let-7 complementary site, the nucleotides that paired to nucleotides 3 and 5 of the miRNA were substituted (see Author's map), using the Quikchange site-directed mutagenesis kit (Stratagene). The UTR fragment was then cloned back into the vector containing the Hmga2 cDNA (pCMV•SPORT6.1). To construct the mammalian expression vectors, Hmga2 inserts were excised from the pCMV•SPORT6.1 constructs using EcoRI restriction sites and cloned into pcDNA3.1 (Invitrogen).
Full sequence of wild-type plasmid available at Addgene plasmid #14789.
Proper citation: RRID:Addgene_14792 Copy
Species: Mus musculus
Genetic Insert: Hmga2 m2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17322030
Comments: The 3' UTR of the mouse Hmga2 cDNA (BC052158) was subcloned into pCR2.1-TOPO (Invitrogen) for site-directed mutagenesis. To disrupt each let-7 complementary site, the nucleotides that paired to nucleotides 3 and 5 of the miRNA were substituted (see Author's map), using the Quikchange site-directed mutagenesis kit (Stratagene). The UTR fragment was then cloned back into the vector containing the Hmga2 cDNA (pCMV•SPORT6.1). To construct the mammalian expression vectors, Hmga2 inserts were excised from the pCMV•SPORT6.1 constructs using EcoRI restriction sites and cloned into pcDNA3.1 (Invitrogen).
Full sequence of wild-type plasmid available at Addgene plasmid #14789.
Proper citation: RRID:Addgene_14790 Copy
Species: Mus musculus
Genetic Insert: Smad7
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pBabe puro 3; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10791980
Comments: See Author's map for more information.
Proper citation: RRID:Addgene_14836 Copy
Species: Mus musculus
Genetic Insert: Sprouty-2 shRNA
Vector Backbone Description: Backbone Size:7566; Vector Backbone:pSico; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17369402
Comments: The sequence of the Spry-2 shRNA is 5'-GCCGG GTTGT CGTTG TAAA-3' and corresponds to nucleotides 1150–1168 of mSpry-2.
Proper citation: RRID:Addgene_14809 Copy
Species: Mus musculus
Genetic Insert: K-ras 3'UTR
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4045; Vector Backbone:pRL-TK; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17401365
Proper citation: RRID:Addgene_14804 Copy
Species: Mus musculus
Genetic Insert: Dicer shRNA
Vector Backbone Description: Backbone Marker:OligoEngine; Backbone Size:6350; Vector Backbone:pSUPER.retro.puro; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17401365
Comments: shRNA sequence: GATCCCCCTG CCTCACTTGA CCTGAATTCA AGAGATTCAG GTCAAGTGAG GCAGTTTTTG GAAC
Proper citation: RRID:Addgene_14779 Copy
Species: Mus musculus
Genetic Insert: Dicer shRNA
Vector Backbone Description: Backbone Marker:OligoEngine; Backbone Size:6350; Vector Backbone:pSUPER.retro.puro; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17401365
Comments: shRNA sequence: GATCCCCGCA TGGTGGTGTC GATATTTTCA AGAGAAATAT CGACACCACC ATGCTTTTTG GAAC
Proper citation: RRID:Addgene_14778 Copy
Species: Mus musculus
Genetic Insert: PERP
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3-Basic; Vector Types:Bacterial Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14707288
Comments: The pPERPlucFL vector was constructed by insertion of a splice acceptor upstream of the luciferase cassette in pGL3Basic and insertion of a BamHI Perp genomic fragment (from -3850 to +4150) into the polylinker region.
Proper citation: RRID:Addgene_14811 Copy
Species: Mus musculus
Genetic Insert: metastasis-associated gene family, member 2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:Mouse Targeting; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_140959 Copy
Species: Mus musculus
Genetic Insert: methyl-CpG binding protein 3
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:Mouse Targeting; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_140958 Copy
Species: Mus musculus
Genetic Insert: metastasis associated 3
Vector Backbone Description: Backbone Marker:Hendrich Lab; Backbone Size:6348; Vector Backbone:pCAG-A3XFiBsd; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31036553
Proper citation: RRID:Addgene_140962 Copy
Species: Mus musculus
Genetic Insert: PI3K catalytic subunit p110alpha-Myc- (iSH2-p85alpha)
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pSRalpha; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please note that NcoI is not a unique enzyme and will cut the plasmid DNA at five locations.
Proper citation: RRID:Addgene_1410 Copy
Species: Mus musculus
Genetic Insert: PI3K regulatory subunit p55alpha
Vector Backbone Description: Backbone Size:3900; Vector Backbone:pCR2.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: does not express, only to subclone into other plasmid, contains internal BglII site
Proper citation: RRID:Addgene_1409 Copy
Species: Mus musculus
Genetic Insert: p19 ARF shRNA -2
Vector Backbone Description: Backbone Marker:Available from Addgene (#11795); Backbone Size:8000; Vector Backbone:pLL3.7; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12853964
Comments: p19 ARF shRNA expressed from a lentiviral vector. The p19 ARF shRNA together with the H1 promoter was cloned from pSIRIP-puro into pLL3.7. Therefore, although the shRNA is normally expressed from the U6 promoter of pLL3.7, p19-ARF shRNA is expressed here from the H1 promoter.
Proper citation: RRID:Addgene_14091 Copy
Species: Mus musculus
Genetic Insert: goosecoid
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pWZL Blast; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17142318
Proper citation: RRID:Addgene_14096 Copy
Species: Mus musculus
Genetic Insert: goosecoid
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pWZL Blast; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17142318
Proper citation: RRID:Addgene_14095 Copy
Species: Mus musculus
Genetic Insert: ERK1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: T7 tag is MASMTGGQQMG.
Made by Sejeong Shin.
Proper citation: RRID:Addgene_14440 Copy
Species: Mus musculus
Genetic Insert: modified U6 promoter (U6lox), U6 STOP cassette, shA1 gene
Vector Backbone Description: Backbone Marker:Rene Maehr; Backbone Size:3000; Vector Backbone:pBS-cas2; Vector Types:Mammalian Expression, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15870264
Comments: inducible shRNA expression vector. shRNA coding region can be replaced as follows: design ~80 bp oligos with 5’ BamHI site followed by mutant loxP, where the last 5 bp are replaced by the first 5 bp of shRNA sequence, followed by the remaining shRNA sequence, hairpin and as shRNA sequence, T stretch and SalI site.
Proper citation: RRID:Addgene_14422 Copy
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