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Species: Homo sapiens
Genetic Insert: Keratin 8
Vector Backbone Description: Backbone Size:0; Vector Backbone:pMRB101; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11788583
Proper citation: RRID:Addgene_18057 Copy
Genetic Insert: Tobacco rattle virus RNA1
Vector Backbone Description: Vector Backbone:pLX-Z4; Vector Types:Plant Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:35332696
Proper citation: RRID:Addgene_180515 Copy
Species: Homo sapiens
Genetic Insert: Glut4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20354141
Proper citation: RRID:Addgene_18087 Copy
Species: Anthozoa
Genetic Insert: Zsgreen
Vector Backbone Description: Backbone Size:6900; Vector Backbone:pSICO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18371425
Comments: HIV-Zsgreen was cloned by removing the U6-TATAlox-CMVie-EGFP-TATAlox- WPRE content of pSICO (Ventura et al., 2004), and adding the EF1-alpha promoter, a multiple cloning site (MCS), an internal ribosome entry site (IRES) and Zsgreen. cDNA can be cloned into the MCS (NotI, EcoRI, HpaI, XbaI, SmaI, and BamHI sites) to enable bicistronic expression with Zsgreen.
Proper citation: RRID:Addgene_18121 Copy
Species: MuLV
Genetic Insert: Gag
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14617360
Comments: This is a YFP-fusion to the amino acids PQ at the C-terminus of the Gag NC domain. This was cloned by replacing Pol with YFP in pMDoldGag-Pol.
Proper citation: RRID:Addgene_1813 Copy
Species: Rattus norvegicus
Genetic Insert: lysosome associated membrane protein 1
Vector Backbone Description: Backbone Marker:BD Biosciences / Clontech; Backbone Size:4700; Vector Backbone:pEYFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:14617360
Comments: Lamp-1 sequence contains a D50E mutation, which is not important for function of plasmid. There is also a silent mutation at bp#715 compared to GenBank NM_012857.1
Proper citation: RRID:Addgene_1816 Copy
Species: Rattus norvegicus
Genetic Insert: lysosome associated membrane protein 1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:Modified Clontech Plasmid; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:14617360
Comments: Lamp-1 sequence contains a D50E mutation, which is not important for function of plasmid. There is also a silent mutation at bp#715 compared to GenBank NM_012857.1
Proper citation: RRID:Addgene_1817 Copy
Species: Mus musculus
Genetic Insert: yes kinase
Vector Backbone Description: Backbone Marker:gift from Japan; Backbone Size:6200; Vector Backbone:pMIK-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11278422
Comments: The vector pMIK-Neo is a gift from Dr. Kazuo Maruyama of the University of Tokyo, Tokyo, Japan. The map of the vector will be sent with the DNA
Proper citation: RRID:Addgene_18067 Copy
Species: Homo sapiens
Genetic Insert: Human Epididymis Protein 4
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_18101 Copy
Species: Homo sapiens
Genetic Insert: Secretory Leucocyte Protease Inhibitor
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_18103 Copy
Species: evolved fluorescent protein
Genetic Insert: mAmetrine1.1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad/His B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18425137
Comments: Improved photostability (~1.5x better) compared to mAmetrine (Addgene plasmid 18083)
Proper citation: RRID:Addgene_18084 Copy
Species: Bacteriophage T4
Genetic Insert: T4 Lysozyme
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop.
Note that the T4 Lysozyme insert sequence provided by the depositing laboratory differs from GenBank reference sequence NP_049736.1 at two amino acids: R12G and I137R. These differences were confirmed by Addgene's sequencing results.
Proper citation: RRID:Addgene_18110 Copy
Species: Bacteriophage T4
Genetic Insert: T4 Lysozyme
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_18111 Copy
Species: SARS-CoV-2
Genetic Insert: S-protein B.1.1.529
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_180774 Copy
Species: Homo sapiens
Genetic Insert: c-MYC
Vector Backbone Description: Backbone Marker:Dr. Robert Hawley of George Washington University; Backbone Size:5660; Vector Backbone:MSCV IRES GFP; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Comments: The MSCV vector is described in; Hawley, R.G., Lieu, F.H.L., Fong, A.Z.C., and Hawley, T.S. Versatile retroviral vectors for potential use in gene therapy. Gene Ther. 1: 136-138, 1994.
Use of the construct is described in; Park, I., Zhao, R., West, J.A., Yabuuchi, A., Huo, H., Ince, T.A., Lerou, P.H., Lensch, M.W., and Daley, G.Q. Reprogramming of human somatic cells to pluripotency with defined factors. Nature, 451, 141-146, 2008.
Please note that the assembled sequence presented here is not 100% reflective of the actual sequence. See the Addgene diagnostic digest (found under the Resource Information section of the plasmid page) for additional information on the expected plasmid size. Addgene has found another HindIII site at the end of the MYC gene (see our quality control sequence).
Proper citation: RRID:Addgene_18119 Copy
Species: Homo sapiens
Genetic Insert: PD-1
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34734802
Proper citation: RRID:Addgene_180819 Copy
Species: Homo sapiens
Genetic Insert: MMLVgag-3xNES-ABE8e
Vector Backbone Description: Backbone Size:4757; Vector Backbone:pCDNA3-like; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35021064
Comments: the backbone was amplified from BIC-Gag-CAS9
(Plasmid #119942)
Proper citation: RRID:Addgene_181751 Copy
Species: Homo sapiens
Genetic Insert: CD82
Vector Backbone Description: Backbone Marker:BD Biosciences / Clontech; Backbone Size:4700; Vector Backbone:pECFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:14617360
Proper citation: RRID:Addgene_1818 Copy
Species: Homo sapiens
Genetic Insert: CD82
Vector Backbone Description: Backbone Marker:BD Biosciences / Clontech; Backbone Size:4700; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:14617360
Proper citation: RRID:Addgene_1819 Copy
Species: Synthetic
Genetic Insert: bacterial codon optimized SpRY
Vector Backbone Description: Vector Backbone:pET28; Vector Types:Bacterial Expression, in vitro transcription; T7 promoter; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36203014
Proper citation: RRID:Addgene_181743 Copy
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