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Species: Saccharomyces cerevisiae
Genetic Insert: Booster-PKA
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5384; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: Booster PKA was first described in https://pubmed.ncbi.nlm.nih.gov/32101394/ and was a gift from Michiyuki Matsuda (Addgene plasmid # 138373 ; http://n2t.net/addgene:138373 ; RRID:Addgene_138373).
Proper citation: RRID:Addgene_171121 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Booster-PKA (from #138373)
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5039; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: Booster PKA was first described in https://pubmed.ncbi.nlm.nih.gov/32101394/ and was a gift from Michiyuki Matsuda (Addgene plasmid # 138373 ; http://n2t.net/addgene:138373 ; RRID:Addgene_138373).
Proper citation: RRID:Addgene_171119 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Rnt1
Vector Backbone Description: Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33751023
Proper citation: RRID:Addgene_171551 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TdTomato, GAL4
Vector Backbone Description: Vector Backbone:pBlueScriptSK; Vector Types:Mammalian Expression, Xenopus laevis expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25385606
Comments: Additional references:
-Koster et. al, Dev Biol. 2001, 233(2):329-46. doi.org/10.1006/dbio.2001.0242
-Bestman, J.E., and Cline, H.T. PNAS. 2008, 105: 20494-20499. doi.org/10.1073/pnas.0806296105
-Gabmrill et. al, J Neurophysiol. 2018, 119: 1947–1961. doi.org/10.1152/jn.00051.2018
Proper citation: RRID:Addgene_161712 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA library targeting yeast genes
Vector Backbone Description: Vector Backbone:ampl43; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33247197
Proper citation: RRID:Addgene_161829 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: OSH2
Vector Backbone Description: Vector Backbone:eGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18077555
Proper citation: RRID:Addgene_161987 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: P(PGK1)-citrine and P(HIS4)-mCherry
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3500; Vector Backbone:pCR™Blunt II-TOPO®; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33303588
Comments: Digesting this plasmid with BciVI leaves compatible Gibson homology arms on either side of divergent promoter expression cassette, allowing direct Gibson assembly into the barcoded-gRNA plasmid library. See manuscript for additional details.
Proper citation: RRID:Addgene_164917 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FCU1
Vector Backbone Description: Backbone Size:2955; Vector Backbone:pUPD2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34525356
Comments: CDS
Proper citation: RRID:Addgene_165842 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PDR12
Vector Backbone Description: Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA Binding Coordinates: chrXVI:449415..449434 (+ strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome
Proper citation: RRID:Addgene_166093 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: LHS1
Vector Backbone Description: Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA Binding Coordinates: chrXI:296533..296552 (- strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome
Proper citation: RRID:Addgene_166092 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL7
Vector Backbone Description: Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA Binding Coordinates: chrII:275406..275425 (+ strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome
Proper citation: RRID:Addgene_166089 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Promoter of TDH3
Vector Backbone Description: Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA Binding Coordinates: chrVII:883938..883957 (- strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome
Proper citation: RRID:Addgene_166084 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PPE1
Vector Backbone Description: Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA Binding Coordinates: chrVIII:249554..249573 (- strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome
Proper citation: RRID:Addgene_166083 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TDH2
Vector Backbone Description: Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA Binding Coordinates: chrX:454530..454549 (- strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome
Proper citation: RRID:Addgene_166082 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Promoter of YNR071C
Vector Backbone Description: Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA Binding Coordinates: chrXIV:771638..771657 (+ strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome
Proper citation: RRID:Addgene_166081 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: LYS9
Vector Backbone Description: Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA Binding Coordinates: chrXIV:715092..715111 (+ strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome
Proper citation: RRID:Addgene_166085 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GLK1
Vector Backbone Description: Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA Binding Coordinates: chrIII:51377..51396 (+ strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome
Proper citation: RRID:Addgene_166090 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hta2-sg18
Vector Backbone Description: Vector Backbone:pYTK102; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_166111 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: APL3
Vector Backbone Description: Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA Binding Coordinates: chrII: 149461-149480 (+ strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome
Proper citation: RRID:Addgene_166073 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Intergenic site near HXT6 (in YDR344C, possible dubious ORF)
Vector Backbone Description: Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA Binding Coordinates: chrIV: 1162101-1162120 (+ strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome
Proper citation: RRID:Addgene_166072 Copy
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