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Species: Synthetic
Genetic Insert: colEdes14/Imdes14
Vector Backbone Description: Backbone Size:5440; Vector Backbone:pET21d; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30538236
Comments: Two genes in tandem (colE, Im), separated by 2 nucleotides (AA). See partial sequences. AA sequence of colEdes14: MESKRNKPGKATGKGKPVGDKWLDDAGKDSGAPIPDRIADKLRDKEFKNFDDFKRKFWEEVSKDPDLSKQFKGSNQNRIKQGQAPFARKKDQVGGRKTFELHHDKPISQDGGVYDMNNIRVTTPKRHIDIHRGK and AA sequence of Imdes14: MELKHSISDYTEAEFLEFVKKICEGHFEAGSDGPKVVQEFERLTEHPDGSDLIYYPRDDREDSPEGIVKEIKEWRAANGKSGFKQGLEHHHHHH
Proper citation: RRID:Addgene_107122 Copy
Species: Synthetic
Genetic Insert: colEdes13/Imdes13
Vector Backbone Description: Backbone Size:5440; Vector Backbone:pET21d; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30538236
Comments: Two genes in tandem (colE, Im), separated by 2 nucleotides (AA). See partial sequences. AA sequence of colEdes13: MESKRNKPGKATGKGKPVGDKWLDDAGKDSGAPIPDRIADKLRDKEFKNFDDFRKKFWEEVSKDPDLSKQFKRSNQNNIKQGIAPFARSKDQVGGRRTFELHHDKPISQDGGVYDMNNIRVTTPKRHIDIHRGK and AA sequence of Imdes13: MELKHSISDYTEAEFLEFVKKIMASNDETQQRKLVTEFERLTEHPDGSDLIYYPRDDREDSPEGIVKEIKEWRAANGKSGFKQGLEHHHHHH
Proper citation: RRID:Addgene_107121 Copy
Species: Synthetic
Genetic Insert: M. sedula PCNA1
Vector Backbone Description: Backbone Marker:Merck Millipore; Backbone Size:5700; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27228945
Proper citation: RRID:Addgene_107063 Copy
Species: Synthetic
Genetic Insert: YC3.6
Vector Backbone Description: Vector Backbone:pGreenII 0179; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18284694
Proper citation: RRID:Addgene_107061 Copy
Species: Synthetic
Genetic Insert: M. sedula PCNA3
Vector Backbone Description: Backbone Marker:Takara Bio; Backbone Size:2700; Vector Backbone:pMD20; Vector Types:Cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27228945
Proper citation: RRID:Addgene_107075 Copy
Species: Synthetic
Genetic Insert: M. sedula PCNA2
Vector Backbone Description: Backbone Marker:Takara Bio; Backbone Size:2700; Vector Backbone:pMD20; Vector Types:Cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27228945
Proper citation: RRID:Addgene_107074 Copy
Species: Synthetic
Genetic Insert: M. sedula PCNA1
Vector Backbone Description: Backbone Marker:Takara Bio; Backbone Size:2700; Vector Backbone:pMD20; Vector Types:Cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27228945
Proper citation: RRID:Addgene_107073 Copy
Species: Synthetic
Genetic Insert: YPet-CyPet fusion protein
Vector Backbone Description: Backbone Marker:Merck Millipore; Backbone Size:5700; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27228945
Proper citation: RRID:Addgene_107072 Copy
Species: Synthetic
Genetic Insert: DNA ligase 1
Vector Backbone Description: Backbone Marker:Merck Millipore; Backbone Size:5400; Vector Backbone:pET-28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27228945
Proper citation: RRID:Addgene_107071 Copy
Species: Synthetic
Genetic Insert: S. solfataricus PCNA3
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:5670; Vector Backbone:pMAL-c5E; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27228945
Proper citation: RRID:Addgene_107069 Copy
Species: Synthetic
Genetic Insert: colEdes2/Imdes2
Vector Backbone Description: Backbone Size:5440; Vector Backbone:pET21d; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30538236
Comments: Two genes in tandem (colE, Im), separated by 2 nucleotides (AA). See partial sequences. AA sequence of colEdes2: MESKRNKPGKATGKGKPVGDKWLDDAGKDSGAPIPDRIADKLRDKEFKNFDDFRKKFWKEVAKDPDLAKQFKRANRRNIKQGRAPFAPKKDQVGGRRTFELHHDKPISQDGGVYDMNNIRVTTPKRHIDIHRGK and AA sequence of Imdes2: MELKHSISDYTEAEFLEFVKKIYNSTDEEKQKELVTEFERLTEHPSGSDLIYYPRDDREDSPEGIVKEIKEWRAANGKSGFKQGLEHHHHHH
Proper citation: RRID:Addgene_107109 Copy
Species: Synthetic
Genetic Insert: F37A+L196F+V252A+C253G-hADPRM GST-mutant cADPR phosphohydrolase gene
Vector Backbone Description: Backbone Marker:GE Healthcare; Backbone Size:4946; Vector Backbone:pGEX-6P-3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29348648
Proper citation: RRID:Addgene_107163 Copy
Species: Synthetic
Genetic Insert: PLL 2.4
Vector Backbone Description: Vector Backbone:pETMBPH; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30018322
Proper citation: RRID:Addgene_107205 Copy
Species: Synthetic
Genetic Insert: PLL 2.2
Vector Backbone Description: Vector Backbone:pETMBPH; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30018322
Proper citation: RRID:Addgene_107203 Copy
Species: Synthetic
Genetic Insert: xyl3.1
Vector Backbone Description: Vector Backbone:pETMBPH; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30018322
Proper citation: RRID:Addgene_107209 Copy
Species: Synthetic
Genetic Insert: PLL 4.1
Vector Backbone Description: Vector Backbone:pETMBPH; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30018322
Proper citation: RRID:Addgene_107208 Copy
Species: Synthetic
Genetic Insert: PLL 3.1
Vector Backbone Description: Vector Backbone:pETMBPH; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30018322
Proper citation: RRID:Addgene_107207 Copy
Species: Synthetic
Genetic Insert: GFP + sgRNA for GFP
Vector Backbone Description: Vector Backbone:pXPR_047; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA sequence is GGTGAACCGCATCGAGCTGA
pLKO TRC v2 library vector, Puro selection and eGFP expression.
Proper citation: RRID:Addgene_107145 Copy
Species: Synthetic
Genetic Insert: xyl8.2
Vector Backbone Description: Vector Backbone:pETMBPH; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30018322
Proper citation: RRID:Addgene_107216 Copy
Species: Synthetic
Genetic Insert: ecAT3.10
Vector Backbone Description: Backbone Marker:modified from Invitrogen; Backbone Size:5000; Vector Backbone:pCMV(MinDis) [variant of pDisplay]; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29121644
Proper citation: RRID:Addgene_107215 Copy
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