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On page 146 showing 2901 ~ 2920 out of 32,424 results
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  • RRID:Addgene_118857

    This resource has 1+ mentions.

http://www.addgene.org/118857

Species: Other
Genetic Insert: ECFP
Vector Backbone Description: Vector Backbone:pQLinkHD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30339749

Proper citation: RRID:Addgene_118857 Copy   


http://www.addgene.org/118840

Vector Backbone Description: Vector Backbone:MiniCoopR; Vector Types:CRISPR, Tol2; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30385465
Comments: Use the BseRI enzyme to clone gRNA of interest in the U6:gRNA cassette. Use primer: CCATACCACATTTGTAGAGGT to sequence insert

Proper citation: RRID:Addgene_118840 Copy   


  • RRID:Addgene_1188

    This resource has 1+ mentions.

http://www.addgene.org/1188

Species: Homo sapiens
Genetic Insert: htt 103Q
Vector Backbone Description: Backbone Size:0; Vector Backbone:p426 GAL; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10677504
Comments: N terminal region of Ht with polyQ repeat length of 103 fused to GFP.

Proper citation: RRID:Addgene_1188 Copy   


  • RRID:Addgene_118803

    This resource has 1+ mentions.

http://www.addgene.org/118803

Species: Synthetic
Genetic Insert: Synthetic
Vector Backbone Description: Vector Backbone:pCS2-Dest; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31653829

Proper citation: RRID:Addgene_118803 Copy   


  • RRID:Addgene_119021

    This resource has 1+ mentions.

http://www.addgene.org/119021

Species: Homo sapiens
Genetic Insert: death-domain associated protein (Daxx)
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23405218

Proper citation: RRID:Addgene_119021 Copy   


  • RRID:Addgene_118956

    This resource has 1+ mentions.

http://www.addgene.org/118956

Species: Rattus norvegicus
Genetic Insert: GABRA5
Vector Backbone Description: Vector Backbone:pEGFP N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin

Proper citation: RRID:Addgene_118956 Copy   


  • RRID:Addgene_118942

    This resource has 1+ mentions.

http://www.addgene.org/118942

Species: Mus musculus
Genetic Insert: OptoTGFBRs
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3925; Vector Backbone:ptdToamto-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29241005

Proper citation: RRID:Addgene_118942 Copy   


  • RRID:Addgene_118943

    This resource has 1+ mentions.

http://www.addgene.org/118943

Species: Homo sapiens
Genetic Insert: iRFP682-Smad2
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:piRFP682-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29241005

Proper citation: RRID:Addgene_118943 Copy   


  • RRID:Addgene_119052

    This resource has 1+ mentions.

http://www.addgene.org/119052

Genetic Insert: A0201-Mart1-SABR
Vector Backbone Description: Backbone Marker:Donald B. Kohn's laboratory at UCLA; Backbone Size:6600; Vector Backbone:pCCLc-MND; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30700902

Proper citation: RRID:Addgene_119052 Copy   


  • RRID:Addgene_119034

    This resource has 1+ mentions.

http://www.addgene.org/119034

Species: Synthetic
Genetic Insert: Voltron-ST
Vector Backbone Description: Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31371562
Comments: 5' cloning site: NheI, 3' cloning site: HindIII

Proper citation: RRID:Addgene_119034 Copy   


  • RRID:Addgene_11915

    This resource has 1+ mentions.

http://www.addgene.org/11915

Genetic Insert: VSVG
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9288971
Comments: The EGFP (Clontech Laboratories, Inc., Palo, Alto, CA) contains a valine immediately after the start codon that is not found in the wild type sequence. However, to avoid confusion with previously published work on GFP mutants, the wild type residue numbers are used. The Kozak sequence at the beginning of the GFP encoding region of the pEGFP-N1 plasmid from Clontech has been disrupted. Reference for the A206K mutation is Zacharias, D. A., Violin, J. D., Newton, A. C., and Tsien, R. Y. (2002) Science 296, 913-6. Reference for PAGFP is Patterson, G. H., and Lippincott-Schwartz, J. (2002) Science 297, 1873-7.

Proper citation: RRID:Addgene_11915 Copy   


  • RRID:Addgene_11911

    This resource has 1+ mentions.

http://www.addgene.org/11911

Species: Aequorea victoria
Genetic Insert: pRSETA-PAGFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2827; Vector Backbone:pRSETA; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12228718
Comments: The fluorescent protein encoding region contains one of the three substitutions (A206K) suggested by Dr. Roger Tsien. This mutation disrupts fluorescent protein dimerization even at high concentrations. The mutation does not seem to have much effect on the fluorescent properties of the CFP, GFP and YFP versions, so hopefully it will not affect PAGFP too much. The PAGFP A206K mutant does not display obvious fluorescent differences compared with the original, but please bear in mind that I haven't characterized it to the same extent. The citation for the A206K mutation in fluorescent proteins is Science 2002 296:913.

Proper citation: RRID:Addgene_11911 Copy   


  • RRID:Addgene_11910

    This resource has 10+ mentions.

http://www.addgene.org/11910

Species: Aequorea victoria
Genetic Insert: PAGFP
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12228718
Comments: The pPAGFP-C1 was not published. It was prepared by removing the PAGFP cDNA from pPAGFP-N1 with an AgeI/BsrGI restriction digest and subcloning it into a similarly digested pEYFP-C1 (Clontech). The pPAGFP-C1 was confirmed by sequencing. If you use this construct in future publications, use your best judgement for citation. The fluorescent protein encoding region contains one of the three substitutions (A206K) suggested by Dr. Roger Tsien. This mutation disrupts fluorescent protein dimerization even at high concentrations. The mutation does not seem to have much effect on the fluorescent properties of the CFP, GFP and YFP versions, so hopefully it will not affect PAGFP too much. The PAGFP A206K mutant does not display obvious fluorescent differences compared with the original, but please bear in mind that I haven't characterized it to the same extent. The citation for the A206K mutation in fluorescent proteins is Science 2002 296:913.

Proper citation: RRID:Addgene_11910 Copy   


  • RRID:Addgene_119129

    This resource has 1+ mentions.

http://www.addgene.org/119129

Species: Other
Genetic Insert: mCherry and eGFP
Vector Backbone Description: Backbone Size:9990; Vector Backbone:plenti-CMV-mCherry-T2A-GFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30679582

Proper citation: RRID:Addgene_119129 Copy   


  • RRID:Addgene_119078

    This resource has 1+ mentions.

http://www.addgene.org/119078

Species: Homo sapiens
Genetic Insert: PIS
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:4700; Vector Backbone:mCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23263280

Proper citation: RRID:Addgene_119078 Copy   


  • RRID:Addgene_11909

    This resource has 1+ mentions.

http://www.addgene.org/11909

Species: Aequorea victoria
Genetic Insert: pPAGFP-N1
Vector Backbone Description: Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12228718
Comments: The fluorescent protein encoding region contains one of the three substitutions (A206K) suggested by Dr. Roger Tsien. This mutation disrupts fluorescent protein dimerization even at high concentrations. The mutation does not seem to have much effect on the fluorescent properties of the CFP, GFP and YFP versions, so hopefully it will not affect PAGFP too much. The PAGFP A206K mutant does not display obvious fluorescent differences compared with the original, but please bear in mind that I haven't characterized it to the same extent. The citation for the A206K mutation in fluorescent proteins is Science 2002 296:913.

Proper citation: RRID:Addgene_11909 Copy   


http://www.addgene.org/11906

Species: Caenorhabditis elegans
Genetic Insert: lin-41 miRNA target sequence
Vector Backbone Description: Backbone Marker:Available at Addgene (plasmid 12178); Backbone Size:5264; Vector Backbone:pIS0; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14697198
Comments: Used to test effects of lin-41 microRNA (let-7).

Proper citation: RRID:Addgene_11906 Copy   


  • RRID:Addgene_11918

    This resource has 1+ mentions.

http://www.addgene.org/11918

Species: bacteriophage P1
Genetic Insert: cre
Vector Backbone Description: Backbone Size:6600; Vector Backbone:na; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10334853
Comments: pBS513 carries the wt cre gene under the control of the elongation factor-1 alpha (EF-1 alpha) promoter. The construct carries an upstream Kozak modification to increase expression. The resulting construct provides very strong expression in many different types of cells, including murine embryonic stem (ES) cells. For cell culture work this construct is recommended as a replacement for the CMV-cre construct pBS185 as it provides much stronger expression. However, pBS513 is not suitable for use in transgenic mice as such EF-1 alpha transgene constructs are often inactive in many tissues. Please note: The following enzymes are NOT single cutters: PstI, AflIII, and SacI. We do not suggest using these enzymes for cloning.

Proper citation: RRID:Addgene_11918 Copy   


  • RRID:Addgene_119271

    This resource has 1+ mentions.

http://www.addgene.org/119271

Species: Other
Genetic Insert: dcas9
Vector Backbone Description: Vector Backbone:R6Kgamma; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30617347

Proper citation: RRID:Addgene_119271 Copy   


  • RRID:Addgene_11925

    This resource has 1+ mentions.

http://www.addgene.org/11925

Genetic Insert: STOP
Vector Backbone Description: Backbone Size:3806; Vector Backbone:n/a; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8231893
Comments: pBS302 carries two directly repeated loxP sites flanking a synthetic DNA sequence designated "STOP." The lox-square STOP cassette sits on a NotI fragment that can excised and gel-purified for injection into fertilized zygotes. The STOP sequence is designed to thwart productive expression of a downstream gene under the control of an upstream promoter (to be inserted in the SfiI-SpeI polylinker region). It will have been removed in cells expressing Cre, or in descendants of cells that previously had expressed Cre, because of Cre-mediated recombination at the loxP sites. The STOP sequence is the same as used to regulate T-Ag expression in pBS241.

Proper citation: RRID:Addgene_11925 Copy   



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