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Species: Saccharomyces cerevisiae
Genetic Insert: YIp ADE2-LEU2 pTDH3::lys1-V26D-GFP::tADH1
Vector Backbone Description: Vector Backbone:pRH3022; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37729018
Proper citation: RRID:Addgene_210147 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YIp ADE2-LEU2 pTDH3::lys1-W353R-GFP::tADH1
Vector Backbone Description: Vector Backbone:pRH3022; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37729018
Proper citation: RRID:Addgene_210145 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YIp ADE2-LEU2 pTDH3::lys1-W151E-GFP::tADH1
Vector Backbone Description: Vector Backbone:pRH3022; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37729018
Proper citation: RRID:Addgene_210152 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YIp ADE2-LEU2 pTDH3::lys1-W151L-GFP::tADH1
Vector Backbone Description: Vector Backbone:pRH3022; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37729018
Proper citation: RRID:Addgene_210156 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YIp ADE2-LEU2 pTDH3::lys1-W151M-GFP::tADH1
Vector Backbone Description: Vector Backbone:pRH3022; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37729018
Proper citation: RRID:Addgene_210185 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ubc13
Vector Backbone Description: Vector Backbone:pET21(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38103558
Comments: pHU3010; ribosome binding site and ATG from pQE
Proper citation: RRID:Addgene_212715 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cas9 (codon optimized for human)
Vector Backbone Description: Backbone Size:5700; Vector Backbone:pRS426; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37326023
Proper citation: RRID:Addgene_187971 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RER2
Vector Backbone Description: Vector Backbone:pKG-GW1 ; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25066056
Proper citation: RRID:Addgene_203170 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FCU1
Vector Backbone Description: Backbone Size:2955; Vector Backbone:pUPD2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34525356
Comments: CDS
Proper citation: RRID:Addgene_165842 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PDR12
Vector Backbone Description: Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA Binding Coordinates: chrXVI:449415..449434 (+ strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome
Proper citation: RRID:Addgene_166093 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: LHS1
Vector Backbone Description: Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA Binding Coordinates: chrXI:296533..296552 (- strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome
Proper citation: RRID:Addgene_166092 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL7
Vector Backbone Description: Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA Binding Coordinates: chrII:275406..275425 (+ strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome
Proper citation: RRID:Addgene_166089 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Promoter of TDH3
Vector Backbone Description: Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA Binding Coordinates: chrVII:883938..883957 (- strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome
Proper citation: RRID:Addgene_166084 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PPE1
Vector Backbone Description: Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA Binding Coordinates: chrVIII:249554..249573 (- strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome
Proper citation: RRID:Addgene_166083 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TDH2
Vector Backbone Description: Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA Binding Coordinates: chrX:454530..454549 (- strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome
Proper citation: RRID:Addgene_166082 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Promoter of YNR071C
Vector Backbone Description: Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA Binding Coordinates: chrXIV:771638..771657 (+ strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome
Proper citation: RRID:Addgene_166081 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: LYS9
Vector Backbone Description: Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA Binding Coordinates: chrXIV:715092..715111 (+ strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome
Proper citation: RRID:Addgene_166085 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GLK1
Vector Backbone Description: Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA Binding Coordinates: chrIII:51377..51396 (+ strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome
Proper citation: RRID:Addgene_166090 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hta2-sg18
Vector Backbone Description: Vector Backbone:pYTK102; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_166111 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: APL3
Vector Backbone Description: Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA Binding Coordinates: chrII: 149461-149480 (+ strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome
Proper citation: RRID:Addgene_166073 Copy
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