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Genetic Insert: eGFP
Vector Backbone Description: Backbone Marker:Matthew Bogyo laboratory; Backbone Size:6126; Vector Backbone:Addgene Plasmid #38251 (pET22b-CPDSalI); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19956581
Comments: Encodes untagged eGFP fused to Vibrio cholerae MARTX toxin cysteine protease domain (CPD) to be used as a positive control for protein purification
Proper citation: RRID:Addgene_38257 Copy
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:6153; Vector Backbone:pET22b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19956581
Comments: For protein purification, target protein of interest should be cloned into NdeI and SalI sites to create HA-tagged fusion with Vibrio cholerae MARTX toxin cysteine protease domain (CPD). HA epitope tag will remain on target protein after cleavage.
Proper citation: RRID:Addgene_38253 Copy
Species: Synthetic
Genetic Insert: E2-Crimson
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5603; Vector Backbone:pEF/myc/ER; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19658435
Comments: Vector for targeting E2-Crimson to the ER in mammalian cells.
Substitutions in E2-Crimson relative to DsRed-Express2 are: E32V, Q66F, V71A, V73I, K83L, L85Q, F118L, L150N, I161N, K163M, V175C, Y193H, S197Y
IMPORTANT NOTE: There is an 82-bp intron after the ER signal sequence. The signal sequence will be in frame with the final product.
Proper citation: RRID:Addgene_38770 Copy
Species: Homo sapiens
Genetic Insert: β₂AR
Vector Backbone Description: Vector Backbone:pcDNA3.1-mCFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16687412
Comments: The Lefkowitz Lab has confirmed this plasmid contains the human gene, and not the rat version described in the publication.
Proper citation: RRID:Addgene_38260 Copy
Species: Homo sapiens
Genetic Insert: Rif
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15668168
Comments: human Rif GTPase (RHOF) with N-terminal myc-tag in pcDNA3. Constitutively active point mutation of Q77L
Proper citation: RRID:Addgene_38768 Copy
Species: Drosophila melanogaster
Genetic Insert: zw4
Vector Backbone Description: Backbone Size:8904; Vector Backbone:pUAST; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9012507
Comments: Perrimon Lab plasmid #77
Proper citation: RRID:Addgene_38764 Copy
Species: Mus musculus
Genetic Insert: Tiar
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pLJM60; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22552098
Proper citation: RRID:Addgene_38244 Copy
Species: Drosophila melanogaster
Genetic Insert: dishevelled
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBlueScript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8288125
Comments: Perrimon Lab plasmid #17
Proper citation: RRID:Addgene_38342 Copy
Species: Drosophila melanogaster
Genetic Insert: spitz
Vector Backbone Description: Backbone Size:7855; Vector Backbone:pCaSpeR-4; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1644292
Comments: Perrimon Lab plasmid #10
Proper citation: RRID:Addgene_38340 Copy
Species: Drosophila melanogaster
Genetic Insert: djun
Vector Backbone Description: Backbone Size:8900; Vector Backbone:pCaSpeR-hs; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1696724
Comments: Perrimon Lab plasmid #7
Proper citation: RRID:Addgene_38337 Copy
Species: Mus musculus
Genetic Insert: Septin 7
Vector Backbone Description: Backbone Marker:Ambion; Backbone Size:3130; Vector Backbone:pSilencer 2.0-U6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19043408
Proper citation: RRID:Addgene_38299 Copy
Species: Escherichia coli
Genetic Insert: E. coli polyphosphate kinase
Vector Backbone Description: Backbone Marker:K. Melcher; Vector Backbone:pKM263; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_38333 Copy
Species: Escherichia coli
Genetic Insert: E. coli polyphosphatase
Vector Backbone Description: Backbone Marker:K. Melcher; Vector Backbone:pKM263; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17320430
Comments: See "Bolesch, D.G., Keasling, J.D., 2000. Polyphosphate binding and chain length recognition of Escherichia coli exopolyphosphatase. J. Biol. Chem. 275, 33814–33819" for more information.
Proper citation: RRID:Addgene_38328 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PPX1
Vector Backbone Description: Backbone Marker:K. Melcher; Vector Backbone:pKM263; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16184370
Proper citation: RRID:Addgene_38327 Copy
Species: Homo sapiens
Genetic Insert: ARRDC1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5472; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22315426
Proper citation: RRID:Addgene_38321 Copy
Species: Homo sapiens
Genetic Insert: Next to BRCA1 gene 1 protein
Vector Backbone Description: Backbone Marker:Dr. Toshio Kitamura of the University of Tokyo ; Backbone Size:5800; Vector Backbone:pMXs-IP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21220506
Comments: DNA res. 1, 223-229, (1994)
Proper citation: RRID:Addgene_38283 Copy
Species: Homo sapiens
Genetic Insert: YWHAG
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pTvHR21-SGC; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: http://www.thesgc.org/structures/2B05/
Proper citation: RRID:Addgene_39129 Copy
Genetic Insert: none
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pFB-Sec-NH; Vector Types:Baculovirus; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_39189 Copy
Species: Rattus norvegicus
Genetic Insert: Erk2
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11352917
Proper citation: RRID:Addgene_39222 Copy
Species: Homo sapiens
Genetic Insert: INCENP
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pGTvL1-SGC; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: http://www.thesgc.org/structures/3U23/
Proper citation: RRID:Addgene_39185 Copy
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