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pNK845
 
Resource Report
Resource Website 		RRID:Addgene_219746 mutant of fungal luciferase Neonothopanus nambi Spectinomycin PMID:38253843 Backbone Size:2247; Vector Backbone:Level 0-like; Vector Types:Luciferase, Synthetic Biology; Bacterial Resistance:Spectinomycin I3S, N4T, F11L, I63T, T99P, T192S, A199P 2026-08-15 01:30:02 0
pKI_ΩBBMVP16&WUS
 
Resource Report
Resource Website 		RRID:Addgene_220348 BABY BOOM Arabidopsis thaliana Spectinomycin PMID:38633452 Vector Backbone:pKI1.1R; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:30:05 0
pDONR223-TAF5-FL
 
Resource Report
Resource Website 		RRID:Addgene_209047 TAF5 full length Homo sapiens Spectinomycin PMID:38917794 Vector Backbone:pDONR223; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Spectinomycin 2026-08-15 01:29:44 0
pDONR223-TAF5-∆ex8
 
Resource Report
Resource Website 		RRID:Addgene_209048 TAF5 deltaexon-8 Homo sapiens Spectinomycin PMID:38917794 Vector Backbone:pDONR223; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Spectinomycin 2026-08-15 01:29:45 0
pDONR223-EGFP
 
Resource Report
Resource Website 		RRID:Addgene_209057 EGFP Aequorea victoria Spectinomycin PMID:38917794 Vector Backbone:pDONR223; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Spectinomycin 2026-08-15 01:29:45 0
pYPQ265E6
 
Resource Report
Resource Website 		RRID:Addgene_213469 TadA-CDa-32aa linker-zSpCas9-D10A-2xUGI Spectinomycin PMID:38877035 Please visit https://doi.org/10.1101/2024.02.25.581917 for bioRxiv preprint. Vector Backbone:pYPQ166; Vector Types:Plant Expression, CRISPR, Gateway compatible TadA-CDa-32aa linker-zSpCas9-D10A-2xUGI entry clone; Bacterial Resistance:Spectinomycin 2026-08-15 01:29:47 0
pYPQ265E7
 
Resource Report
Resource Website 		RRID:Addgene_213470 TadA-CDd-32aa linker-zSpCas9-D10A-2xUGI Spectinomycin PMID:38877035 Please visit https://doi.org/10.1101/2024.02.25.581917 for bioRxiv preprint. Vector Backbone:pYPQ166; Vector Types:Plant Expression, CRISPR, Gateway compatible TadA-CDd-32aa linker-zSpCas9-D10A-2xUGI entry clone; Bacterial Resistance:Spectinomycin 2026-08-15 01:29:46 0
pReporter_ZF1_mNeonGreen
 
Resource Report
Resource Website 		RRID:Addgene_213840 mNeonGreen Synthetic Spectinomycin PMID:38906116 Contains a minimal CYC1 promoter driving expression of mNeonGreen. Binding sites for ZF-1 (ZF 43-8) are upstream of the promoter. Cut with NotI prior to transformation into yeast to facilitate genomic insertion. ** This plasmid is prone to recombination due to ZF binding site repeats.** Vector Backbone:pLEU; Vector Types:Yeast Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:29:47 0
pReporter_ZF2_mNeonGreen
 
Resource Report
Resource Website 		RRID:Addgene_213841 mNeonGreen Synthetic Spectinomycin PMID:38906116 Contains a minimal CYC1 promoter driving expression of mNeonGreen. Binding sites for ZF-2 (ZF 42-10) are upstream of the promoter. Cut with NotI prior to transformation into yeast to facilitate genomic insertion. **This plasmid is prone to recombination due to ZF binding site repeats.** Vector Backbone:pLEU; Vector Types:Yeast Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:29:46 0
pPtet-1-VP16
 
Resource Report
Resource Website 		RRID:Addgene_213843 VP16 Synthetic Spectinomycin PMID:38906116 Contains one aTc-inducible promoter variants driving the expression of VP-16 fused to ZF-1 (ZF-43-8). **This plasmid is prone to recombination due to repeats in the aTc-inducible promoters.** Vector Backbone:self-made; Vector Types:Yeast Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:29:47 0
pStorage_Ptet-1_ZF1
 
Resource Report
Resource Website 		RRID:Addgene_213844 Spectinomycin PMID:38906116 Storage plasmid for type 1 expression vectors -- i.e., fusions to ZF-1 (ZF 43-8). To insert barcoded genes: i) digest this plasmid with BbsI and MfeI ii) digest the barcoded gene with BsaI -- making sure the resultant overhangs match the BbsI and MfeI sites iii) ligate the two digestion products. Terminators can be added to the barcoded ligation product via a BbsI golden gate reaction. Vector Backbone:self-made; Vector Types:Yeast Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:29:46 0
pStorage_Ptet-2_ZF2
 
Resource Report
Resource Website 		RRID:Addgene_213845 Spectinomycin PMID:38906116 Storage plasmid for type 1 expression vectors -- i.e., fusions to ZF-2 (ZF 42-10). To insert barcoded genes: i) digest this plasmid with BbsI and MfeI ii) digest the barcoded gene with BsaI -- making sure the resultant overhangs match the BbsI and MfeI sites iii) ligate the two digestion products. Terminators can be added to the barcoded ligation product via a BbsI golden gate reaction. Vector Backbone:self-made; Vector Types:Yeast Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:29:46 0
pReporter_mNeonGreen
 
Resource Report
Resource Website 		RRID:Addgene_213838 mNeonGreen Synthetic Spectinomycin PMID:38906116 Contains a minimal CYC1 promoter driving expression of mNeonGreen. No ZF binding sites are present. Cut with NotI prior to transformation into yeast to facilitate genomic insertion. Vector Backbone:pLEU; Vector Types:Yeast Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:29:46 0
pPtet-1_mNeonGreen_Ptet-2_mCherry
 
Resource Report
Resource Website 		RRID:Addgene_213839 mNeonGreen, mCherry Synthetic Spectinomycin PMID:38906116 Contains two aTc-inducible variants driving the expression of mNeonGreen and mCherry. **This plasmid is prone to recombination due to repeats in the aTc-inducible promoters.** Vector Backbone:self-made; Vector Types:Yeast Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:29:46 0
pReporter_ZF1_mNeonGreen_ZF2
 
Resource Report
Resource Website 		RRID:Addgene_213835 mNeonGreen Synthetic Spectinomycin PMID:38906116 Contains a minimal CYC1 promoter driving expression of mNeonGreen. Binding sites for ZF-1 (ZF 43-8) are upstream of the promoter and ZF-2 (ZF 42-10) binding sites are downstream. Cut with NotI prior to transformation into yeast to facilitate genomic insertion. **This plasmid is prone to recombination due to ZF binding site repeats.** Vector Backbone:pLEU; Vector Types:Yeast Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:29:46 0
pDONR223 ERFlux (Bip-HaloTag7-mGFP-KDEL-STOP)
 
Resource Report
Resource Website 		RRID:Addgene_215002 KDEL Other Spectinomycin Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin 2026-08-15 01:29:50 0
pDONR223 LC3Flux (HaloTag7-mGFP-LC3-STOP)
 
Resource Report
Resource Website 		RRID:Addgene_215004 MAP1LC3B Rattus norvegicus Spectinomycin Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin 2026-08-15 01:29:51 0
GB4468
 
Resource Report
Resource Website 		RRID:Addgene_215257 SF-LUZ-H3H-EGFP-p19 Synthetic Spectinomycin PMID:37882352 Backbone Marker:self-made; derived from pCambia1302 generated at the Cambia Institute; Vector Backbone:pDGB3_omega2; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin BsaI and BsmBI sites removed 2026-08-15 01:29:50 0
GB4665
 
Resource Report
Resource Website 		RRID:Addgene_215270 MAR10-SF-nptII-LUZ-CPH-MAR10-SF Synthetic Spectinomycin PMID:37882352 Backbone Marker:self-made; derived from pCambia1302 generated at the Cambia Institute; Vector Backbone:pDGB3_omega1; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin BsaI and BsmBI sites removed 2026-08-15 01:29:51 0
pBi2FC-C-MGGAGM-P2A:mTagBFP2
 
Resource Report
Resource Website 		RRID:Addgene_216141 MGGAGM Linker Synthetic Spectinomycin PMID:39100877 Please visit https://www.biorxiv.org/content/10.1101/2024.06.28.601222v1.full for bioRxiv preprint. Vector Backbone:pGWB502-omega; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:29:51 0

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