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Species: Neonothopanus nambi
Genetic Insert: mutant of fungal luciferase
Vector Backbone Description: Backbone Size:2247; Vector Backbone:Level 0-like; Vector Types:Luciferase, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38253843
Proper citation: RRID:Addgene_219746 Copy
Species: Arabidopsis thaliana
Genetic Insert: BABY BOOM
Vector Backbone Description: Vector Backbone:pKI1.1R; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38633452
Proper citation: RRID:Addgene_220348 Copy
Species: Homo sapiens
Genetic Insert: TAF5 full length
Vector Backbone Description: Vector Backbone:pDONR223; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38917794
Proper citation: RRID:Addgene_209047 Copy
Species: Homo sapiens
Genetic Insert: TAF5 deltaexon-8
Vector Backbone Description: Vector Backbone:pDONR223; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38917794
Proper citation: RRID:Addgene_209048 Copy
Species: Aequorea victoria
Genetic Insert: EGFP
Vector Backbone Description: Vector Backbone:pDONR223; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38917794
Proper citation: RRID:Addgene_209057 Copy
Genetic Insert: TadA-CDa-32aa linker-zSpCas9-D10A-2xUGI
Vector Backbone Description: Vector Backbone:pYPQ166; Vector Types:Plant Expression, CRISPR, Gateway compatible TadA-CDa-32aa linker-zSpCas9-D10A-2xUGI entry clone; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38877035
Comments: Please visit https://doi.org/10.1101/2024.02.25.581917 for bioRxiv preprint.
Proper citation: RRID:Addgene_213469 Copy
Genetic Insert: TadA-CDd-32aa linker-zSpCas9-D10A-2xUGI
Vector Backbone Description: Vector Backbone:pYPQ166; Vector Types:Plant Expression, CRISPR, Gateway compatible TadA-CDd-32aa linker-zSpCas9-D10A-2xUGI entry clone; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38877035
Comments: Please visit https://doi.org/10.1101/2024.02.25.581917 for bioRxiv preprint.
Proper citation: RRID:Addgene_213470 Copy
Species: Synthetic
Genetic Insert: mNeonGreen
Vector Backbone Description: Vector Backbone:pLEU; Vector Types:Yeast Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38906116
Comments: Contains a minimal CYC1 promoter driving expression of mNeonGreen. Binding sites for ZF-1 (ZF 43-8) are upstream of the promoter. Cut with NotI prior to transformation into yeast to facilitate genomic insertion.
** This plasmid is prone to recombination due to ZF binding site repeats.**
Proper citation: RRID:Addgene_213840 Copy
Species: Synthetic
Genetic Insert: mNeonGreen
Vector Backbone Description: Vector Backbone:pLEU; Vector Types:Yeast Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38906116
Comments: Contains a minimal CYC1 promoter driving expression of mNeonGreen. Binding sites for ZF-2 (ZF 42-10) are upstream of the promoter. Cut with NotI prior to transformation into yeast to facilitate genomic insertion.
**This plasmid is prone to recombination due to ZF binding site repeats.**
Proper citation: RRID:Addgene_213841 Copy
Species: Synthetic
Genetic Insert: VP16
Vector Backbone Description: Vector Backbone:self-made; Vector Types:Yeast Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38906116
Comments: Contains one aTc-inducible promoter variants driving the expression of VP-16 fused to ZF-1 (ZF-43-8).
**This plasmid is prone to recombination due to repeats in the aTc-inducible promoters.**
Proper citation: RRID:Addgene_213843 Copy
Vector Backbone Description: Vector Backbone:self-made; Vector Types:Yeast Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38906116
Comments: Storage plasmid for type 1 expression vectors -- i.e., fusions to ZF-1 (ZF 43-8).
To insert barcoded genes:
i) digest this plasmid with BbsI and MfeI
ii) digest the barcoded gene with BsaI -- making sure the resultant overhangs match the BbsI and MfeI sites
iii) ligate the two digestion products.
Terminators can be added to the barcoded ligation product via a BbsI golden gate reaction.
Proper citation: RRID:Addgene_213844 Copy
Vector Backbone Description: Vector Backbone:self-made; Vector Types:Yeast Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38906116
Comments: Storage plasmid for type 1 expression vectors -- i.e., fusions to ZF-2 (ZF 42-10).
To insert barcoded genes:
i) digest this plasmid with BbsI and MfeI
ii) digest the barcoded gene with BsaI -- making sure the resultant overhangs match the BbsI and MfeI sites
iii) ligate the two digestion products.
Terminators can be added to the barcoded ligation product via a BbsI golden gate reaction.
Proper citation: RRID:Addgene_213845 Copy
Species: Synthetic
Genetic Insert: mNeonGreen
Vector Backbone Description: Vector Backbone:pLEU; Vector Types:Yeast Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38906116
Comments: Contains a minimal CYC1 promoter driving expression of mNeonGreen. No ZF binding sites are present.
Cut with NotI prior to transformation into yeast to facilitate genomic insertion.
Proper citation: RRID:Addgene_213838 Copy
Species: Synthetic
Genetic Insert: mNeonGreen, mCherry
Vector Backbone Description: Vector Backbone:self-made; Vector Types:Yeast Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38906116
Comments: Contains two aTc-inducible variants driving the expression of mNeonGreen and mCherry.
**This plasmid is prone to recombination due to repeats in the aTc-inducible promoters.**
Proper citation: RRID:Addgene_213839 Copy
Species: Synthetic
Genetic Insert: mNeonGreen
Vector Backbone Description: Vector Backbone:pLEU; Vector Types:Yeast Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38906116
Comments: Contains a minimal CYC1 promoter driving expression of mNeonGreen. Binding sites for ZF-1 (ZF 43-8) are upstream of the promoter and ZF-2 (ZF 42-10) binding sites are downstream.
Cut with NotI prior to transformation into yeast to facilitate genomic insertion.
**This plasmid is prone to recombination due to ZF binding site repeats.**
Proper citation: RRID:Addgene_213835 Copy
Species: Other
Genetic Insert: KDEL
Vector Backbone Description: Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_215002 Copy
Species: Rattus norvegicus
Genetic Insert: MAP1LC3B
Vector Backbone Description: Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_215004 Copy
Species: Synthetic
Genetic Insert: SF-LUZ-H3H-EGFP-p19
Vector Backbone Description: Backbone Marker:self-made; derived from pCambia1302 generated at the Cambia Institute; Vector Backbone:pDGB3_omega2; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37882352
Proper citation: RRID:Addgene_215257 Copy
Species: Synthetic
Genetic Insert: MAR10-SF-nptII-LUZ-CPH-MAR10-SF
Vector Backbone Description: Backbone Marker:self-made; derived from pCambia1302 generated at the Cambia Institute; Vector Backbone:pDGB3_omega1; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37882352
Proper citation: RRID:Addgene_215270 Copy
Species: Synthetic
Genetic Insert: MGGAGM Linker
Vector Backbone Description: Vector Backbone:pGWB502-omega; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:39100877
Comments: Please visit https://www.biorxiv.org/content/10.1101/2024.06.28.601222v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_216141 Copy
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