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  • Organism:saccharomyces cerevisiae (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pAAV CaMKII Booster PKA
 
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RRID:Addgene_171121 Booster-PKA Saccharomyces cerevisiae Ampicillin Booster PKA was first described in https://pubmed.ncbi.nlm.nih.gov/32101394/ and was a gift from Michiyuki Matsuda (Addgene plasmid # 138373 ; http://n2t.net/addgene:138373 ; RRID:Addgene_138373). Backbone Marker:Stratagene; Backbone Size:5384; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin 2026-08-15 01:10:13 0
pAAV gfaABC1D Booster PKA
 
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RRID:Addgene_171119 Booster-PKA (from #138373) Saccharomyces cerevisiae Ampicillin Booster PKA was first described in https://pubmed.ncbi.nlm.nih.gov/32101394/ and was a gift from Michiyuki Matsuda (Addgene plasmid # 138373 ; http://n2t.net/addgene:138373 ; RRID:Addgene_138373). Backbone Marker:Stratagene; Backbone Size:5039; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin 2026-08-15 01:10:13 0
pGEX-4T-1-Rnt1-dsRBD(+3A)
 
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RRID:Addgene_171551 Rnt1 Saccharomyces cerevisiae Ampicillin PMID:33751023 Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin a three-alanine insertion in the L1 region 2026-08-15 01:10:18 0
p alpha-actinin:GAL4/UAS:TdTomato
 
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RRID:Addgene_161712 TdTomato, GAL4 Saccharomyces cerevisiae Ampicillin PMID:25385606 Additional references: -Koster et. al, Dev Biol. 2001, 233(2):329-46. doi.org/10.1006/dbio.2001.0242 -Bestman, J.E., and Cline, H.T. PNAS. 2008, 105: 20494-20499. doi.org/10.1073/pnas.0806296105 -Gabmrill et. al, J Neurophysiol. 2018, 119: 1947–1961. doi.org/10.1152/jn.00051.2018 Vector Backbone:pBlueScriptSK; Vector Types:Mammalian Expression, Xenopus laevis expression; Bacterial Resistance:Ampicillin 2026-08-15 01:08:57 0
Yeast Inducible CRISPRi Library
 
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RRID:Addgene_161829 gRNA library targeting yeast genes Saccharomyces cerevisiae Ampicillin PMID:33247197 Vector Backbone:ampl43; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:08:57 0
GFP-OSH2-2xPH
 
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1+ mentions
RRID:Addgene_161987 OSH2 Saccharomyces cerevisiae Kanamycin PMID:18077555 Vector Backbone:eGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:08:58 1
pNTI757 P(PGK1)-citrine P(HIS4)-mCherry
 
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1+ mentions
RRID:Addgene_164917 P(PGK1)-citrine and P(HIS4)-mCherry Saccharomyces cerevisiae Kanamycin PMID:33303588 Digesting this plasmid with BciVI leaves compatible Gibson homology arms on either side of divergent promoter expression cassette, allowing direct Gibson assembly into the barcoded-gRNA plasmid library. See manuscript for additional details. Backbone Marker:Invitrogen; Backbone Size:3500; Vector Backbone:pCR™Blunt II-TOPO®; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:09:25 1
pFCU1
 
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RRID:Addgene_165842 FCU1 Saccharomyces cerevisiae Ampicillin PMID:34525356 CDS Backbone Size:2955; Vector Backbone:pUPD2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:32 0
pNSU299_PDR12
 
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RRID:Addgene_166093 PDR12 Saccharomyces cerevisiae Ampicillin gRNA Binding Coordinates: chrXVI:449415..449434 (+ strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:09:34 0
pNSU299_LHS1
 
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RRID:Addgene_166092 LHS1 Saccharomyces cerevisiae Ampicillin gRNA Binding Coordinates: chrXI:296533..296552 (- strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:09:34 0
pNSU299_GAL7
 
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RRID:Addgene_166089 GAL7 Saccharomyces cerevisiae Ampicillin gRNA Binding Coordinates: chrII:275406..275425 (+ strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:09:34 0
pNSU299_TDH3
 
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RRID:Addgene_166084 Promoter of TDH3 Saccharomyces cerevisiae Ampicillin gRNA Binding Coordinates: chrVII:883938..883957 (- strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:09:34 0
pNSU299_PPE1
 
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RRID:Addgene_166083 PPE1 Saccharomyces cerevisiae Ampicillin gRNA Binding Coordinates: chrVIII:249554..249573 (- strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:09:34 0
pNSU299_TDH2
 
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RRID:Addgene_166082 TDH2 Saccharomyces cerevisiae Ampicillin gRNA Binding Coordinates: chrX:454530..454549 (- strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:09:34 0
pNSU299_YNR071C
 
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RRID:Addgene_166081 Promoter of YNR071C Saccharomyces cerevisiae Ampicillin gRNA Binding Coordinates: chrXIV:771638..771657 (+ strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:09:34 0
pNSU299_LYS9
 
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RRID:Addgene_166085 LYS9 Saccharomyces cerevisiae Ampicillin gRNA Binding Coordinates: chrXIV:715092..715111 (+ strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:09:34 0
pNSU299_GLK1
 
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RRID:Addgene_166090 GLK1 Saccharomyces cerevisiae Ampicillin gRNA Binding Coordinates: chrIII:51377..51396 (+ strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:09:34 0
HCP9
 
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RRID:Addgene_166111 Hta2-sg18 Saccharomyces cerevisiae Kanamycin Vector Backbone:pYTK102; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Kanamycin 2026-08-15 01:09:34 0
pNSU299_APL3
 
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RRID:Addgene_166073 APL3 Saccharomyces cerevisiae Ampicillin gRNA Binding Coordinates: chrII: 149461-149480 (+ strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:09:33 0
pNSU299_YDR344C
 
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RRID:Addgene_166072 Intergenic site near HXT6 (in YDR344C, possible dubious ORF) Saccharomyces cerevisiae Ampicillin gRNA Binding Coordinates: chrIV: 1162101-1162120 (+ strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:09:33 0

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