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Species: bacteriophage P1
Genetic Insert: EGFP-cre
Vector Backbone Description: Backbone Size:6600; Vector Backbone:na; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10334853
Comments: pBS598 carries the EGFPcre fusion gene under the control of the strong elongation factor EF1alpha promoter. This promoter expresses at high levels in most cell types, including embryonic stem (ES) cells. However its use in transgenic mice is not recommended: the promoter is often silenced in a transgene setting. The GFP moiety of the fusion gene carries the S65T mutation for enhanced fluorescence and is codon-optimised for higher level expression.
Note: pBS513 (EF1alpha-cre) is very similar to pBS598 but carries the wt cre gene and thus serves as the non-fluorescent counterpart of pBS598.
Proper citation: RRID:Addgene_11923 Copy
Species: Other
Genetic Insert: sgRNA NT1/RR1 (rfp)
Vector Backbone Description: Vector Backbone:R6Kgamma; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30617347
Proper citation: RRID:Addgene_119250 Copy
Species: Other
Genetic Insert: tnsABCD
Vector Backbone Description: Vector Backbone:R6Kgamma; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30617347
Comments: very similar to pTNS3 (PMID: 18156318)
Please visit https://www.biorxiv.org/content/early/2018/05/05/315499 for BioRxiv preprint.
Proper citation: RRID:Addgene_119239 Copy
Species: Mus musculus
Genetic Insert: Vegfd
Vector Backbone Description: Vector Backbone:psubCMV-WPRE; Vector Types:AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_119226 Copy
Species: Other
Genetic Insert: split cTEVp in fusion with ABI
Vector Backbone Description: Backbone Size:5443; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30531965
Proper citation: RRID:Addgene_119214 Copy
Species: Other
Genetic Insert: split nTEVp in sufion with PYL1
Vector Backbone Description: Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30531965
Proper citation: RRID:Addgene_119213 Copy
Species: Other
Genetic Insert: HyPer3
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4600; Vector Backbone:pAAV-MCS; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30279532
Comments: Can be used for in vivo expression of the hydrogen peroxide-sensitive fluorescent protein HyPer3. It was used for this purpose in Steinhorn et al. Free Radic Biol Med. 2017 Dec;113:16-25. doi: 10.1016/j.freeradbiomed.2017.09.006.
In the associated publication (Steinhorn et al. Nat Commun. 2018 Oct 2;9(1):4044. doi: 10.1038/s41467-018-06533-2), it was used as a control for a virus driving expression of a fusion between HyPer and D-amino acid oxidase.
Proper citation: RRID:Addgene_119183 Copy
Species: R. gracilis
Genetic Insert: HyPer-DAAO-NES
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4600; Vector Backbone:pAAV-MCS; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30279532
Proper citation: RRID:Addgene_119164 Copy
Species: Homo sapiens
Genetic Insert: GalT
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:8670420
Comments: The EGFP (Clontech Laboratories, Inc., Palo, Alto, CA) contains a valine immediately after the start codon that is not found in the wild type sequence. However, to avoid confusion with previously published work on GFP mutants, the wild type residue numbers are used. This construct contains amino acids 1-60 of the human galactosyltransferase. The original article describes cloning into pcDNA1 ro pCDLSRa. A subsequent paper Zaal et. al. Cell 99:589-601 (1999) describes subcloning into the parent vector used here.
Proper citation: RRID:Addgene_11929 Copy
Genetic Insert: CD3delta-YFP
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3961; Vector Backbone:EGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16103128
Comments: CD3 delta was PCR amplified from a plasmid, introducing flanking BglII and EcoRI sites, and cloned in Ub-R-YFP digested with BamHI and EcoRI.
Proper citation: RRID:Addgene_11951 Copy
Genetic Insert: YFP-CL1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3932; Vector Backbone:EGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16103128
Comments: The YFP-CL1 reporter was generated by inserting an oligodimer encoding the 16 amino acid CL1 degradation signal (ACKNWFSSLSHFVIHL) in enhanced GFP-C1 (BD Biosciences Clontech) using the EcoRI and BamH1 restriction sites, then the enhanced GFP open-reading frame was replaced with YFP using the Nhe1 and SspB1 restriction sites.
Proper citation: RRID:Addgene_11950 Copy
Genetic Insert: Ub-M-GFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5830; Vector Backbone:pYES2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14644450
Comments: The ubiquitin open reading frame was amplified by PCR from the Ub-Pro-Gal plasmid with the sense primer 5'-GCG GAATTCACCATGCAGATCTTCGTGAAGACT-3' and the antisense primer 5'-GCG GGATCCTGTCGACCAAGCTTCCCXXX CCCACCTCTGAGACGGAGTAC-3' where the XXX leads to a Methionine at position 1 (see article 10802622, Figure 1). The PCR product was cloned into the EcoRI and BamHI sites of the EGFP-N1 vector from Clontech.
The Ub-M-GFP insert was then excised with EcoRI and NotI and cloned into pYES2.
Proper citation: RRID:Addgene_11952 Copy
Genetic Insert: Ub-G76V-GFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5830; Vector Backbone:pYES2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14644450
Comments: The ubiquitin open reading frame was amplified by PCR from the Ub-Pro-Gal plasmid with the sense primer 5'-GCG GAATTCACCATGCAGATCTTCGTGAAGACT-3' and the antisense primer 5'-GCGGGATCCTGTCGACCAAGCTTC
CCCACCACACCTCTGAGACGGAGTAC-3'. The PCR product was cloned into the EcoRI and BamHI sites of the EGFP-N1 vector from Clontech.
The Ub-G76V-GFP insert was then excised with EcoRI and NotI and cloned into pYES2.
Proper citation: RRID:Addgene_11954 Copy
Species: Homo sapiens
Genetic Insert: GalT
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEYFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:8670420
Comments: Venus was developed by Miyawaki and colleagues. The reference is Nagai, T., Ibata, K., Park, E. S., Kubota, M., Mikoshiba, K., and Miyawaki, A. (2002) Nat Biotechnol 20, 87-90. The EYFP (Clontech Laboratories, Inc., Palo, Alto, CA) contains a valine immediately after the start codon that is not found in the wild type sequence. However, to avoid confusion with previously published work on GFP mutants, the wild type residue numbers are used. This construct contains amino acids 1-60 of the human galactosyltransferase. The original article describes cloning into pcDNA1 ro pCDLSRa. A subsequent paper Zaal et. al. Cell 99:589-601 (1999) describes subcloning into the parent vector used here.
Proper citation: RRID:Addgene_11931 Copy
Species: Homo sapiens
Genetic Insert: GalT
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pECFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:8670420
Comments: Cerulean ws developed by Piston lab. Reference is Rizzo, M. A., Springer, G. H., Granada, B., and Piston, D. W. (2004) Nat Biotechnol 22, 445-9. The EGFP (Clontech Laboratories, Inc., Palo, Alto, CA) contains a valine immediately after the start codon that is not found in the wild type sequence. However, to avoid confusion with previously published work on GFP mutants, the wild type residue numbers are used. This construct contains amino acids 1-60 of the human galactosyltransferase. The original article describes cloning into pcDNA1 ro pCDLSRa. A subsequent paper Zaal et. al. Cell 99:589-601 (1999) describes subcloning into the parent vector used here.
Proper citation: RRID:Addgene_11930 Copy
Species: Homo sapiens
Genetic Insert: GalT
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pECFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:8670420
Comments: This construct contains amino acids 1-60 of the human galactosyltransferase. The original article describes cloning into pcDNA1 or pCDLSRa. A subsequent paper Zaal et. al. Cell 99:589-601 (1999) describes subcloning into the parent vector used here.
Addgene Note: Please see the Addgene sequencing result to view the fluorophore sequence.
Proper citation: RRID:Addgene_11937 Copy
Species: Homo sapiens
Genetic Insert: GalT
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEYFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:8670420
Comments: This construct contains amino acids 1-60 of the human galactosyltransferase. The original article describes cloning into pcDNA1 ro pCDLSRa. A subsequent paper Zaal et. al. Cell 99:589-601 (1999) describes subcloning into the parent vector used here.
Proper citation: RRID:Addgene_11936 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4500; Vector Backbone:EGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16606690
Comments: PAGFP is a photoactivatable version of GFP. It is essentially the same as the standard EGFP-C1 from clontech, but contains the mutations L64F/T65S/V163A/T203H, and conatins an A206K mutation to disrupt dimerization.
In total, the PAGFP-Ubiquitin insert is 983bp.
Proper citation: RRID:Addgene_11933 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin KO.G76V
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:EGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16606690
Proper citation: RRID:Addgene_11932 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4500; Vector Backbone:EGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16702407
Proper citation: RRID:Addgene_11935 Copy
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