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Species: Synthetic
Genetic Insert: xyl8.1
Vector Backbone Description: Vector Backbone:pETMBPH; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30018322
Proper citation: RRID:Addgene_107214 Copy
Species: Synthetic
Genetic Insert: xyl4.2
Vector Backbone Description: Vector Backbone:pETMBPH; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30018322
Proper citation: RRID:Addgene_107213 Copy
Species: Synthetic
Genetic Insert: xyl3.2
Vector Backbone Description: Vector Backbone:pETMBPH; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30018322
Proper citation: RRID:Addgene_107210 Copy
Species: Synthetic
Genetic Insert: 24xPP7v3
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29281824
Proper citation: RRID:Addgene_107230 Copy
Species: Synthetic
Genetic Insert: 1D3-28Z. 1-3 mut
Vector Backbone Description: Backbone Size:5586; Vector Backbone:MSGV1; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20631379
Proper citation: RRID:Addgene_107227 Copy
Species: Synthetic
Genetic Insert: 1D3-28Z All ITAMs intact
Vector Backbone Description: Backbone Size:5584; Vector Backbone:MSGV1; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20631379
Proper citation: RRID:Addgene_107226 Copy
Species: Synthetic
Genetic Insert: SpCas9-SaCas9 fusion
Vector Backbone Description: Vector Backbone:pCSDest2; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30451839
Proper citation: RRID:Addgene_107319 Copy
Species: Synthetic
Genetic Insert: SpCas9-SaCas9 fusion
Vector Backbone Description: Vector Backbone:pCSDest2; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30451839
Proper citation: RRID:Addgene_107318 Copy
Species: Synthetic
Genetic Insert: SpCas9-NmCas9 fusion
Vector Backbone Description: Vector Backbone:pCSDest2; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30451839
Proper citation: RRID:Addgene_107326 Copy
Species: Synthetic
Genetic Insert: SpCas9-NmCas9 fusion
Vector Backbone Description: Vector Backbone:pCSDest2; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30451839
Proper citation: RRID:Addgene_107325 Copy
Species: Synthetic
Genetic Insert: SpCas9-NmCas9 fusion
Vector Backbone Description: Vector Backbone:pCSDest2; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30451839
Proper citation: RRID:Addgene_107324 Copy
Species: Synthetic
Genetic Insert: SpCas9-SaCas9 fusion
Vector Backbone Description: Vector Backbone:pCSDest2; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30451839
Proper citation: RRID:Addgene_107322 Copy
Species: Synthetic
Genetic Insert: SpCas9-SaCas9 fusion
Vector Backbone Description: Vector Backbone:pCSDest2; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30451839
Proper citation: RRID:Addgene_107320 Copy
Species: Synthetic
Genetic Insert: HU-FRB
Vector Backbone Description: Backbone Size:4313; Vector Backbone:pBR322; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29357135
Proper citation: RRID:Addgene_107255 Copy
Species: Synthetic
Genetic Insert: ProA-RBS(B0032)-Gemini (E0051)
Vector Backbone Description: Backbone Marker:http://parts.igem.org/Part:pSB3C5; Backbone Size:2738; Vector Backbone:pSB3C5; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:20843779
Proper citation: RRID:Addgene_107241 Copy
Species: Synthetic
Genetic Insert: Full length LexA repressor
Vector Backbone Description: Backbone Size:6363; Vector Backbone:pSB4K5; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28946740
Proper citation: RRID:Addgene_107237 Copy
Species: Synthetic
Genetic Insert: BASU
Vector Backbone Description: Vector Backbone:pLEX; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29400715
Comments: The BASU protein sequence has 4 mutations relative to wild type Bacillus subtilis biotin ligase (Uniprot P0CI75): N terminal truncation of 65 aa, R124G, E323S and G325R.
Proper citation: RRID:Addgene_107250 Copy
Species: Synthetic
Genetic Insert: hM3DREADD
Vector Backbone Description: Backbone Size:6597; Vector Backbone:AAV2; Vector Types:AAV; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/early/2018/01/22/251769 for bioRxiv preprint.
These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.
Proper citation: RRID:Addgene_107246 Copy
Species: Synthetic
Genetic Insert: Rncp-iGluSnFR1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5268; Vector Backbone:pDisplay; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29308878
Comments: Please note that mutation M178K in the Neomycin resistance cassette was found during Addgene's quality control. Neomycin selection has not been tested for this plasmid, but should not affect sensor function.
Proper citation: RRID:Addgene_107336 Copy
Species: Synthetic
Genetic Insert: SpyCatcher002-oDi
Vector Backbone Description: Backbone Size:3648; Vector Backbone:pDEST14; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30988307
Proper citation: RRID:Addgene_124661 Copy
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