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Species: Homo sapiens
Genetic Insert: Halotag
Vector Backbone Description: Vector Backbone:pUC57-BsaI-Free; Vector Types:Donor vector; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_178139 Copy
Species: Homo sapiens
Genetic Insert: ATP1A1 G3 + EBFP nick sgRNAs
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR, Prime editing; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36207338
Comments: Control PE3 nick sgRNAs vector derived from ATP1A1_G3_Dual_sgRNA. This vector can be used as a positive control for prime editing (PE3) to co-target ATP1A1 (Q118R) and EBFP to perform coselection for EBFP to EGFP conversion. Please visit https://doi.org/10.1101/2021.11.02.464583 for bioRxiv preprint.
Proper citation: RRID:Addgene_178094 Copy
Species: Homo sapiens
Genetic Insert: Halotag
Vector Backbone Description: Vector Backbone:pUC57-BsaI-Free; Vector Types:Donor vector; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_178132 Copy
Species: Homo sapiens
Genetic Insert: NPM1 G6 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
Vector Backbone Description: Vector Backbone:pX330-U6-Chimeric_BB-CBh-hSpCas9; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36207338
Comments: This plasmid is derived from eSpCas9(1.1) (Plasmid #71814) from Feng Zhang's lab. The 3x FLAG sequence was removed to prevent interference of the tag in western blot and immunoprecipitation applications. Please visit https://doi.org/10.1101/2021.11.02.464583 for bioRxiv preprint.
Proper citation: RRID:Addgene_178091 Copy
Species: Homo sapiens
Genetic Insert: Halotag
Vector Backbone Description: Vector Backbone:pUC57-BsaI-Free; Vector Types:Donor vector; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_178125 Copy
Species: Homo sapiens
Genetic Insert: Halotag
Vector Backbone Description: Vector Backbone:pUC57-BsaI-Free; Vector Types:Donor vector; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_178126 Copy
Species: Homo sapiens
Genetic Insert: Halotag
Vector Backbone Description: Vector Backbone:pUC57-BsaI-Free; Vector Types:Donor vector; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_178123 Copy
Species: Homo sapiens
Genetic Insert: Halotag
Vector Backbone Description: Vector Backbone:pUC57-BsaI-Free; Vector Types:Donor vector; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_178124 Copy
Species: Homo sapiens
Genetic Insert: EGFP
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36207338
Comments: Plasmid derived from AAVS1_Puro_PGK1_3xFLAG_Twin_Strep (Addgene 68375). Please visit https://doi.org/10.1101/2021.11.02.464583 for bioRxiv preprint.
Proper citation: RRID:Addgene_178088 Copy
Species: Homo sapiens
Genetic Insert: Halotag
Vector Backbone Description: Vector Backbone:pUC57-BsaI-Free; Vector Types:Donor vector; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_178121 Copy
Species: Homo sapiens
Genetic Insert: EBFP (T65S/F145Y)
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36207338
Comments: Plasmid derived from AAVS1_Puro_PGK1_3xFLAG_Twin_Strep (Addgene 68375). Please visit https://doi.org/10.1101/2021.11.02.464583 for bioRxiv preprint.
Proper citation: RRID:Addgene_178089 Copy
Species: Homo sapiens
Genetic Insert: Halotag
Vector Backbone Description: Vector Backbone:pUC57-BsaI-Free; Vector Types:Donor vector; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_178122 Copy
Species: Homo sapiens
Genetic Insert: mScarlet-I mTOR signaling indicator (TOSI)
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36207338
Comments: Plasmid derived from AAVS1_Puro_PGK1_3xFLAG_Twin_Strep (Addgene 68375). Please visit https://doi.org/10.1101/2021.11.02.464583 for bioRxiv preprint.
Proper citation: RRID:Addgene_178087 Copy
Species: Homo sapiens
Genetic Insert: Halotag
Vector Backbone Description: Vector Backbone:pUC57-BsaI-Free; Vector Types:Donor vector; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_178120 Copy
Species: Homo sapiens
Genetic Insert: Halotag
Vector Backbone Description: Vector Backbone:pUC57-BsaI-Free; Vector Types:Donor vector; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_178140 Copy
Species: Homo sapiens
Genetic Insert: Halotag
Vector Backbone Description: Vector Backbone:pUC57-BsaI-Free; Vector Types:Donor vector; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_178141 Copy
Species: Homo sapiens
Genetic Insert: Toll-Interacting Protein
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFPc3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32311122
Proper citation: RRID:Addgene_178050 Copy
Species: Homo sapiens
Genetic Insert: Toll-Interacting Protein
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5300; Vector Backbone:pIRESneo2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32311122
Proper citation: RRID:Addgene_178054 Copy
Species: Homo sapiens
Genetic Insert: nPC Tagged mCherry-NLS
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pLKO; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35290801
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.07.13.451021v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_178235 Copy
Species: Homo sapiens
Genetic Insert: nPC Tagged mCherry-NLS
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pLKO; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35290801
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.07.13.451021v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_178234 Copy
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