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Species: Saccharomyces cerevisiae
Genetic Insert: Intergenic region near CPR1
Vector Backbone Description: Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA Binding Coordinates: chrIV: 768328-768347 (+ strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome
Addgene QC identified a G>C difference at the terminal nucleotide of the gRNA compared to the expected sequence. The depositing lab does not know if this difference is of functional consequence.
Proper citation: RRID:Addgene_166071 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YBL055C (near PTC3)
Vector Backbone Description: Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA Binding Coordinates: chrII: 115606-115625 (- strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome
Proper citation: RRID:Addgene_166077 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Whi5-sg106
Vector Backbone Description: Vector Backbone:pYTK102; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_166110 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SEO1
Vector Backbone Description: Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA Binding Coordinates: chrI: 7457-7476 (+ strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome
Proper citation: RRID:Addgene_166076 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CPR1
Vector Backbone Description: Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA Binding Coordinates: chrIV: 768512-769000 (+ strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome
Proper citation: RRID:Addgene_166074 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: IDH2 sgRNA #7
Vector Backbone Description: Vector Backbone:pROS13; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.05.25.445643v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_166102 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: IDH1 sgRNA #4
Vector Backbone Description: Vector Backbone:pROS13; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.05.25.445643v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_166101 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cyr1CT-sgRNA
Vector Backbone Description: Vector Backbone:pYTK103; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_166107 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cyr1Del-sgRNA/X1-sgRNA
Vector Backbone Description: Vector Backbone:pYTK102; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_166106 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Msn2-sgRNA#27
Vector Backbone Description: Vector Backbone:pYTK102; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_166104 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Whi5-sg106
Vector Backbone Description: Vector Backbone:pYTK102; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_166109 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cyr1CT-sgRNA/X1-sgRNA
Vector Backbone Description: Vector Backbone:pYTK103; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_166108 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VP2C-GFP
Vector Backbone Description: Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34591448
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.01.30.428974v1 for BioRxiv preprint
Proper citation: RRID:Addgene_166674 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Wild-type Murine polyomavirus VP1
Vector Backbone Description: Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34591448
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.01.30.428974v1 for BioRxiv preprint
Proper citation: RRID:Addgene_166673 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: UDH
Vector Backbone Description: Vector Backbone:pUG73; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34591448
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.01.30.428974v1 for BioRxiv preprint
Proper citation: RRID:Addgene_166679 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VP2C-GFP
Vector Backbone Description: Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34591448
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.01.30.428974v1 for BioRxiv preprint
Proper citation: RRID:Addgene_166676 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VAC8
Vector Backbone Description: Vector Backbone:pRS405; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32508216
Comments: Linearize with AflII
Proper citation: RRID:Addgene_166837 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VAC8
Vector Backbone Description: Vector Backbone:pRS405; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32508216
Comments: Linearize with AflII
Proper citation: RRID:Addgene_166836 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VAC8
Vector Backbone Description: Vector Backbone:pRS405; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32508216
Comments: Linearize with AflII
Proper citation: RRID:Addgene_166839 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Atg13[571–700]
Vector Backbone Description: Vector Backbone:pRS424; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31352862
Proper citation: RRID:Addgene_166853 Copy
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