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Vector Backbone Description: Backbone Size:2644; Vector Backbone:puc19; Vector Types:Zebrafish transgenesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25628360
Comments: Zebrafish transgenesis vector: tol1 arms, QUAS-E1b promoter, ubiquitin C intron, multiple cloning site, ocean pout antifreeze protein terminator.
Co-inject with tol1 (not tol2) transposase.
Proper citation: RRID:Addgene_62216 Copy
Genetic Insert: Fok1-dCas9
Vector Backbone Description: Backbone Marker:gift from Silvia Aldaz; Backbone Size:12429; Vector Backbone:pact; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: Please visit crisprflydesign.org for more information.
Please acknowledge Fillip Port and Simon Bullock when publishing work derived from the use of this plasmid.
Proper citation: RRID:Addgene_62211 Copy
Species: Synthetic
Genetic Insert: 3xFLAG-dCas9
Vector Backbone Description: Backbone Size:5385; Vector Backbone:p414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36325175
Comments: This plasmid is compatible with gRNA expression plasmids for budding yeast, such as Addgene Plasmid #43803 ( http://www.addgene.org/43803 ).
Proper citation: RRID:Addgene_62190 Copy
Species: Synthetic
Genetic Insert: VP64dCas9BFPVP64
Vector Backbone Description: Vector Backbone:TetO-FUW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25448066
Comments: The vector backbone was from Addgene plasmid 27152 (PI Marius Wernig)
The dCas9:BFP fusion part of the insert was cloned from Addgene plasmid 44247 (PI Stanley Qi)
Proper citation: RRID:Addgene_62195 Copy
Genetic Insert: human RHD_exon1_TALEN_Right
Vector Backbone Description: Backbone Size:3739; Vector Backbone:pcDNA3; Vector Types:TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26078220
Proper citation: RRID:Addgene_62240 Copy
Species: Synthetic
Genetic Insert: A2T sgRNA
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25422271
Proper citation: RRID:Addgene_62258 Copy
Species: Synthetic
Genetic Insert: A1NT
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25422271
Proper citation: RRID:Addgene_62255 Copy
Species: Synthetic
Genetic Insert: A1T sgRNA
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25422271
Proper citation: RRID:Addgene_62256 Copy
Vector Backbone Description: Backbone Marker:Structural Genomics Consortium; Backbone Size:6767; Vector Backbone:pFBOH-MHL; Vector Types:Baculovirus expression; Bacterial Resistance:Ampicillin
Comments: The pFBOH-MHL vector is a derivative of the pFBOH-LIC vector (SGC). It is a donor vector for generation of recombinant baculovirus by site-specific transposition in an E. coli host. This vector adds an 18 amino acid N-terminal fusion tag containing a 6X His followed by a TEV cleavage site. Two stop codons are included in the vector at the C-terminal cloning site.
Insertion of a DNA sequence into the cloning/expression region is performed using Clontech’s In-fusion enzyme mediated directional recombination between complementary 15 nucleotide DNA sequences at the ends of the insert (PCR product) and BseRI linearized vector. Insertion of a target sequence involves replacement of a SacB gene stuffer sequence, which provides for negative selection of the original plasmid on 5% sucrose.
Proper citation: RRID:Addgene_62304 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: OSM1-EGFP fusion
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25378625
Proper citation: RRID:Addgene_62386 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: OSM1-EGFP fusion
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25378625
Proper citation: RRID:Addgene_62387 Copy
Species: Other
Genetic Insert: mCherry-BirA
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:yeast c-terminal tagging vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25378625
Proper citation: RRID:Addgene_62388 Copy
Species: Synthetic
Genetic Insert: su9-tagBFP
Vector Backbone Description: Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25378625
Proper citation: RRID:Addgene_62383 Copy
Genetic Insert: sgRNA + 2x PP7 RNA binding module
Vector Backbone Description: Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25533786
Proper citation: RRID:Addgene_62317 Copy
Genetic Insert: sgRNA + 1x com RNA binding module
Vector Backbone Description: Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25533786
Proper citation: RRID:Addgene_62318 Copy
Genetic Insert: sgRNA + 1x MS2 binding module
Vector Backbone Description: Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25533786
Proper citation: RRID:Addgene_62313 Copy
Genetic Insert: sgRNA + 2x MS2 binding module
Vector Backbone Description: Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25533786
Proper citation: RRID:Addgene_62314 Copy
Species: Synthetic
Genetic Insert: MluI-XhoI-HindIII-PstI-SalI MCS
Vector Backbone Description: Backbone Marker:Neveu lab, Addgene plasmid #62394; Backbone Size:1945; Vector Backbone:MXS_chaining vector; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25909630
Proper citation: RRID:Addgene_62397 Copy
Species: Synthetic
Genetic Insert: MluI-XhoI-PvuII-BglII-SalI MCS
Vector Backbone Description: Backbone Marker:Neveu lab, Addgene plasmid #62394; Backbone Size:1945; Vector Backbone:MXS_chaining vector; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25909630
Proper citation: RRID:Addgene_62398 Copy
Vector Backbone Description: Backbone Size:1945; Vector Backbone:Custom Backbone; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25909630
Proper citation: RRID:Addgene_62394 Copy
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