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Species: Synthetic
Genetic Insert: SpyCatcher002-oTri
Vector Backbone Description: Backbone Size:3648; Vector Backbone:pDEST14; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30988307
Proper citation: RRID:Addgene_124662 Copy
Species: Synthetic
Genetic Insert: Venus-Akaluciferase
Vector Backbone Description: Backbone Marker:Eric Campeau & Paul Kaufman; Backbone Size:9780; Vector Backbone:pLenti PGK Neo DEST (Addgene #19067); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33241215
Proper citation: RRID:Addgene_124701 Copy
Species: Synthetic
Genetic Insert: jRGECO1a
Vector Backbone Description: Backbone Size:9800; Vector Backbone:fCAG-WPRE; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30952812
Proper citation: RRID:Addgene_124775 Copy
Species: Synthetic
Genetic Insert: CheRiff-GFP
Vector Backbone Description: Backbone Size:5100; Vector Backbone:AAV-CAG-FLEX; Vector Types:AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30952812
Proper citation: RRID:Addgene_124776 Copy
Species: Synthetic
Genetic Insert: hSPCas9
Vector Backbone Description: Backbone Size:9200; Vector Backbone:SpCas9(BB)-2A-GFP (PX458); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31489367
Proper citation: RRID:Addgene_124808 Copy
Species: Synthetic
Genetic Insert: cis-aconitate decarboxylase
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3519; Vector Backbone:pCOLADuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31548418
Proper citation: RRID:Addgene_124842 Copy
Species: Synthetic
Genetic Insert: SPOON
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29963852
Proper citation: RRID:Addgene_124840 Copy
Species: Synthetic
Genetic Insert: PyronicSF
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3097; Vector Backbone:pBI-CMV1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32142409
Comments: Please visit https://www.biorxiv.org/content/10.1101/611806v1 for BioRxiv preprint
Proper citation: RRID:Addgene_124830 Copy
Species: Synthetic
Genetic Insert: mTagBFP2-T2A-FLPo
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: In the NGS sequence, polymorphisms are present in a GC-rich region of the Chicken beta-actin promoter that do not affect the function of the plasmid.
Proper citation: RRID:Addgene_124834 Copy
Species: Synthetic
Genetic Insert: pac-T2A-FLPo
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_124835 Copy
Species: Synthetic
Genetic Insert: pac-T2A-dre
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_124833 Copy
Species: Synthetic
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pMCSV; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26969085
Comments: Guide RNA to be cloned into BbsI site. Supplied modified golden-gate cloning method is required given that extra BbsI site is located within the 2A sequence.
To design guide RNAs:
-add ACCG to 5' end of positive strand (positive strand defined as strand which will contact PAM sequence at 3' end)
-add AAAC to 5' end of negative strand
Proper citation: RRID:Addgene_124889 Copy
Species: Synthetic
Genetic Insert: Nanoluc, copGFP
Vector Backbone Description: Vector Backbone:pCDH-EF1 (Addgene #72266); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_124979 Copy
Species: Synthetic
Genetic Insert: mCherry-GFP-LC3B
Vector Backbone Description: Vector Backbone:pCDH-CMV (Addgene #72265) ; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29368982
Proper citation: RRID:Addgene_124974 Copy
Species: Synthetic
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:AV4-EF1-GFP; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22027994
Proper citation: RRID:Addgene_124971 Copy
Species: Synthetic
Genetic Insert: dsRed
Vector Backbone Description: Backbone Marker:BCM; Backbone Size:24000; Vector Backbone:pD21; Vector Types:Adenoviral; Bacterial Resistance:Ampicillin
Comments: As this plasmid is very large, NGS had difficulty sequencing it. There are several differences between Addgene's NGS and depositors sequence. We recommend running additional diagnostic tests before use.
Depositor also recommends growing plasmid at 30 degrees C due to their size.
Proper citation: RRID:Addgene_124977 Copy
Species: Synthetic
Genetic Insert: Nanoluc, copGFP
Vector Backbone Description: Vector Backbone:pCDH-EF1 (Addgene #72266); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_124978 Copy
Species: Synthetic
Genetic Insert: copGFP
Vector Backbone Description: Backbone Marker:BCM; Backbone Size:24000; Vector Backbone:pD21; Vector Types:Adenoviral; Bacterial Resistance:Ampicillin
Comments: As this plasmid is very large, NGS had difficulty sequencing it. There are several differences between Addgene's NGS and depositor's sequence. We recommend running additional diagnostic tests before use.
Depositor also recommends growing plasmid at 30 degrees C due to their size.
Proper citation: RRID:Addgene_124963 Copy
Species: Synthetic
Genetic Insert: dCas9-VP64
Vector Backbone Description: Backbone Size:6457; Vector Backbone:pBac[3xP3-DsRed]; Vector Types:Insect Expression, piggyback; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34078888
Proper citation: RRID:Addgene_125002 Copy
Species: Synthetic
Genetic Insert: dCas9-VP64
Vector Backbone Description: Backbone Size:6457; Vector Backbone:pBac[3xP3-DsRed]; Vector Types:Insect Expression, piggyback; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34078888
Proper citation: RRID:Addgene_125001 Copy
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