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Species: Homo sapiens
Genetic Insert: PCNA Homology Arms with mEGFP-linker
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 to add a mEGFP tag to the N-terminus of human PCNA in WTC-11 human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: GEGQGQGQGPGRGYAYRS. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ .
Proper citation: RRID:Addgene_170818 Copy
Species: Homo sapiens
Genetic Insert: ERGIC-53
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4026; Vector Backbone:pDendra2-C; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35051356
Comments: Please see the Genbank file under Resource Information for an annotated sequence file from the depositing laboratory.
Proper citation: RRID:Addgene_170717 Copy
Species: Homo sapiens
Genetic Insert: STAT5A
Vector Backbone Description: Vector Backbone:pTRIPZ; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34263728
Comments: A constitutively active form of STAT5A was cloned into the lentiviral expression vector.
Proper citation: RRID:Addgene_170662 Copy
Species: Homo sapiens
Genetic Insert: W96A-GLTP
Vector Backbone Description: Vector Backbone:pET30 Xa/LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15329726
Proper citation: RRID:Addgene_170734 Copy
Species: Homo sapiens
Genetic Insert: GLTP
Vector Backbone Description: Vector Backbone:pSUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30206120
Proper citation: RRID:Addgene_170732 Copy
Species: Homo sapiens
Genetic Insert: GLTP
Vector Backbone Description: Vector Backbone:pET30 Xa/LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15329726
Proper citation: RRID:Addgene_170733 Copy
Species: Homo sapiens
Genetic Insert: K60A-CPTP
Vector Backbone Description: Vector Backbone:pSUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23863933
Proper citation: RRID:Addgene_170736 Copy
Species: Homo sapiens
Genetic Insert: E. coli ornithine transcarbamylase (OTC), fused to human ubiquitin
Vector Backbone Description: Backbone Marker:Novagen (+ 6xHis-TEV in-house modification); Backbone Size:3810; Vector Backbone:pRSFDuet1-6xHis-TEV; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33719392
Proper citation: RRID:Addgene_170729 Copy
Species: Homo sapiens
Genetic Insert: Synaptotagmin-like 5
Vector Backbone Description: Backbone Marker:Clonetech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34172739
Proper citation: RRID:Addgene_170868 Copy
Species: Homo sapiens
Genetic Insert: Human GABAA receptor alpha1 subunit
Vector Backbone Description: Vector Backbone:pIRES2-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30481215
Proper citation: RRID:Addgene_170820 Copy
Species: Homo sapiens
Genetic Insert: Human GABAA receptor alpha1 subunit carrying A322D mutation
Vector Backbone Description: Vector Backbone:pIRES2-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30481215
Proper citation: RRID:Addgene_170821 Copy
Species: Homo sapiens
Genetic Insert: RE8
Vector Backbone Description: Vector Backbone:pRF TpC; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33852895
Proper citation: RRID:Addgene_171047 Copy
Species: Homo sapiens
Genetic Insert: RE7
Vector Backbone Description: Vector Backbone:pRF TpC; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33852895
Proper citation: RRID:Addgene_171046 Copy
Species: Homo sapiens
Genetic Insert: RE5
Vector Backbone Description: Vector Backbone:pRF TpC; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33852895
Proper citation: RRID:Addgene_171044 Copy
Species: Homo sapiens
Genetic Insert: RE4
Vector Backbone Description: Vector Backbone:pRF TpC; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33852895
Proper citation: RRID:Addgene_171043 Copy
Species: Homo sapiens
Genetic Insert: RE2
Vector Backbone Description: Vector Backbone:pRF TpC; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33852895
Proper citation: RRID:Addgene_171041 Copy
Species: Homo sapiens
Genetic Insert: RE1
Vector Backbone Description: Vector Backbone:pRF TpC; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33852895
Proper citation: RRID:Addgene_171040 Copy
Species: Homo sapiens
Genetic Insert: Fyn
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34161759
Proper citation: RRID:Addgene_171035 Copy
Species: Homo sapiens
Genetic Insert: LAT
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34161759
Proper citation: RRID:Addgene_171032 Copy
Species: Homo sapiens
Genetic Insert: EZH2
Vector Backbone Description: Vector Backbone:pX330; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32632336
Proper citation: RRID:Addgene_171031 Copy
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