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Species: Caenorhabditis elegans
Genetic Insert: tbb-2 gld-1 operon
Vector Backbone Description: Vector Backbone:pDONRP2-P3R; Vector Types:Worm targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22290181
Proper citation: RRID:Addgene_34875 Copy
Genetic Insert: NeoR
Vector Backbone Description: Vector Backbone:pDONRP2-P3R; Vector Types:Worm targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22290181
Proper citation: RRID:Addgene_34878 Copy
Species: Homo sapiens
Genetic Insert: PIMT
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5400; Vector Backbone:pET30b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18318686
Proper citation: RRID:Addgene_34852 Copy
Genetic Insert: PSmOrange2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5390; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22900938
Comments: There is a single nucleotide deletion in Addgene's SV40pA-R sequence corresponding to position 2766 of full plasmid sequence. It does not alter the ORF or any restriction sites.
https://www.ncbi.nlm.nih.gov/nuccore/JQ392581
Proper citation: RRID:Addgene_34961 Copy
Genetic Insert: PSmOrange2
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22900938
Comments: https://www.ncbi.nlm.nih.gov/nuccore/JQ392581 There are D26G and V119I amino acid changes in Addgene's quality control sequence for H2B. It does not alter the function of this plasmid.
Proper citation: RRID:Addgene_34960 Copy
Genetic Insert: PSmOrange2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5677; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22900938
Comments: https://www.ncbi.nlm.nih.gov/nuccore/JQ392581
Proper citation: RRID:Addgene_34964 Copy
Genetic Insert: PSmOrange2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5328; Vector Backbone:pC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22900938
Comments: In Addgene's CMV-F sequencing result, there is a 6 nucleotide deletion corresponding to position 595 of the full plasmid sequence. It is before the ORF and does not appear alter any restriction sites or the function of the plasmid.
https://www.ncbi.nlm.nih.gov/nuccore/JQ392581
Proper citation: RRID:Addgene_34963 Copy
Genetic Insert: PSmOrange2
Vector Backbone Description: Backbone Size:5275; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22900938
Comments: https://www.ncbi.nlm.nih.gov/nuccore/JQ392581
In Addgene's SV40pA-R sequencing result, there is one mismatch and a two nucleotide deletion corresponding to position 2652 of the full plasmid sequence. This results in a loss of the NotI site but does not alter the function of the plasmid.
Proper citation: RRID:Addgene_34962 Copy
Genetic Insert: PSmOrange2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4013; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22900938
Comments: https://www.ncbi.nlm.nih.gov/nuccore/JQ392581
Proper citation: RRID:Addgene_34958 Copy
Genetic Insert: Lck-GCaMP5G
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3950; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23035093
Comments: Lck-GCaMP5G is a fusion between the Lck domain (https://www.ncbi.nlm.nih.gov/pubmed/21205365) and cytosolic GCaMP5G (http://www.addgene.org/31788). This construct targets GCaMP5G to the plasma membrane.
Proper citation: RRID:Addgene_34924 Copy
Species: Mus musculus
Genetic Insert: focal adhesion kinase
Vector Backbone Description: Backbone Marker:BD Clontech; Backbone Size:4700; Vector Backbone:pmCherry-c1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20150423
Comments: Please note that both the full plasmid sequence and Addgene's sequencing result identified a Y42H mutation when compared to GenBank entry NP_032008.2.
The final two FAK amino acids are missing in this construct, as the original source for the FAK construct used in this plasmid was originally missing these same amino acids. The depositing laboratory states that the deletion should not affect any FAK function since it is outside of the FAK primary sequence.
Proper citation: RRID:Addgene_35040 Copy
Species: Homo sapiens
Genetic Insert: FEN1DELTAP
Vector Backbone Description: Backbone Marker:Stratgene; Backbone Size:7500; Vector Backbone:pShuttle; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20551483
Proper citation: RRID:Addgene_35030 Copy
Species: Mus musculus
Genetic Insert: FEN1mWT
Vector Backbone Description: Backbone Marker:Stratgene; Backbone Size:7500; Vector Backbone:pShuttle; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20551483
Proper citation: RRID:Addgene_35033 Copy
Species: Homo sapiens
Genetic Insert: TIN2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:7500; Vector Backbone:pCMVShuttle; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20097687
Proper citation: RRID:Addgene_35025 Copy
Species: Homo sapiens
Genetic Insert: FEN1DA
Vector Backbone Description: Backbone Marker:Stratgene; Backbone Size:7500; Vector Backbone:pShuttle; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20551483
Proper citation: RRID:Addgene_35028 Copy
Genetic Insert: SpyTagMBP
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22366317
Proper citation: RRID:Addgene_35050 Copy
Species: Homo sapiens
Genetic Insert: Rab5a
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22072788
Proper citation: RRID:Addgene_35140 Copy
Species: Homo sapiens
Genetic Insert: Rab5a
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4722; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22072788
Proper citation: RRID:Addgene_35139 Copy
Species: Homo sapiens
Genetic Insert: Rab5a
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4722; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22072788
Proper citation: RRID:Addgene_35138 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:3637; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000048; Origin: p15A
Proper citation: RRID:Addgene_35282 Copy
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