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Species: Homo sapiens
Genetic Insert: EZH2
Vector Backbone Description: Vector Backbone:puc19; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29891558
Proper citation: RRID:Addgene_171108 Copy
Species: Homo sapiens
Genetic Insert: hTERT FL-Firefly
Vector Backbone Description: Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33852895
Proper citation: RRID:Addgene_171057 Copy
Species: Homo sapiens
Genetic Insert: hTERT dEx2-nanoLuc
Vector Backbone Description: Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33852895
Proper citation: RRID:Addgene_171056 Copy
Species: Homo sapiens
Genetic Insert: pTERT sgRNA2(MS2) mCherry
Vector Backbone Description: Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33852895
Proper citation: RRID:Addgene_171055 Copy
Species: Homo sapiens
Genetic Insert: sgRNA(MS2) mCherry
Vector Backbone Description: Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33852895
Proper citation: RRID:Addgene_171053 Copy
Species: Homo sapiens
Genetic Insert: MS2-p65-HSF1_sfGFP
Vector Backbone Description: Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33852895
Proper citation: RRID:Addgene_171051 Copy
Species: Homo sapiens
Genetic Insert: Puromycin
Vector Backbone Description: Vector Backbone:pTopo pLox hPGK-Puro pA pLox; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33852895
Proper citation: RRID:Addgene_171050 Copy
Species: Homo sapiens
Genetic Insert: SUZ12
Vector Backbone Description: Vector Backbone:puc19; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29891558
Proper citation: RRID:Addgene_171110 Copy
Species: Homo sapiens
Genetic Insert: LargeBit-SNAPtag- Beta-actin
Vector Backbone Description: Backbone Marker:System Biosciences; Backbone Size:7657; Vector Backbone:pCDH; Vector Types:Mammalian Expression, Lentiviral, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34140497
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.08.20.258350v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171019 Copy
Species: Homo sapiens
Genetic Insert: U6:sgRNA_Puro-T2A-BFP
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pCRISPRia-v2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34414886
Proper citation: RRID:Addgene_171011 Copy
Species: Homo sapiens
Genetic Insert: OsTIR1-mEmerald-Cdt1-P2A-miniAID-mCherry2
Vector Backbone Description: Backbone Marker:Eric Campeau, Paul Kaufman; Vector Backbone:pLenti CMV Blast DEST (706-1); Vector Types:Mammalian Expression, Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37537305
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.04.23.441203v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171096 Copy
Species: Homo sapiens
Genetic Insert: OsTIR1-mEmerald-P2A-miniAID-mCherry2
Vector Backbone Description: Backbone Marker:Eric Campeau, Paul Kaufman; Vector Backbone:pLenti CMV Blast DEST (706-1); Vector Types:Mammalian Expression, Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37537305
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.04.23.441203v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171094 Copy
Species: Homo sapiens
Genetic Insert: human androgen receptor Ser256Ala mutant
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5500; Vector Backbone:pCIneo; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17043241
Comments: Mutation in an AR Ser-Pro phosphorylation site, and the exact amino acid residue position and composition may slightly vary based on human AR versions.
Proper citation: RRID:Addgene_171227 Copy
Species: Homo sapiens
Genetic Insert: human androgen receptor Ser81Glu mutant
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5500; Vector Backbone:pCIneo; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17043241
Comments: Mutation in an AR Ser-Pro phosphorylation site, and the exact amino acid residue position and composition may slightly vary based on human AR versions.
Proper citation: RRID:Addgene_171224 Copy
Species: Homo sapiens
Genetic Insert: human androgen receptor Ser81Ala mutant
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5500; Vector Backbone:pCIneo; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Mutation in an AR Ser-Pro phosphorylation site, and the exact amino acid residue position and composition may slightly vary based on human AR versions.
Proper citation: RRID:Addgene_171223 Copy
Species: Homo sapiens
Genetic Insert: AR-C619Y
Vector Backbone Description: Backbone Size:10000; Vector Backbone:Lenti-viral-Hygro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34911936
Comments: The mutation occurs at the end of androgen receptor zinc finger 2 and this AR mutant is defective in transactivation but not nuclear import.
Human androgen receptor reference: https://pubmed.ncbi.nlm.nih.gov/18319300/
Please Note: This plasmid contains a 6x Gln deletion in the 23x Gln track and a 8x Gly deletion in the 23x Gly track in androgen receptor compared to the depositor's provided sequence. These differences are not known to affect the function of the protein.
Proper citation: RRID:Addgene_171222 Copy
Species: Homo sapiens
Genetic Insert: AR-R599A-N600A
Vector Backbone Description: Backbone Size:10000; Vector Backbone:Lenti-viral-Hygro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34911936
Comments: The mutations occur in the D-box of the androgen receptor Zinc finger 2 that mediates AR dimerization.
Please Note: This plasmid contains a 6x Gln deletion in the 23x Gln track and a 8x Gly deletion in the 23x Gly track in androgen receptor compared to the depositor's provided sequence. These differences are not known to affect the function of the protein.
Proper citation: RRID:Addgene_171221 Copy
Species: Homo sapiens
Genetic Insert: AR-AAFF
Vector Backbone Description: Backbone Size:10000; Vector Backbone:Lenti-viral-Hygro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34911936
Comments: The mutations occur on the androgen receptor KVFF motif in the P-box of the zinc finger 1 that make contact with DNA. AR mutants of the KVFF motif would lose activity to activate AR-mediated transcription.
Please Note: This plasmid contains a 6x Gln deletion in the 23x Gln track and a 8x Gly deletion in the 23x Gly track in androgen receptor compared to the depositor's provided sequence. These differences are not known to affect the function of the protein.
Proper citation: RRID:Addgene_171218 Copy
Species: Homo sapiens
Genetic Insert: M-SPOTIT1.1
Vector Backbone Description: Backbone Size:6783; Vector Backbone:PLX208; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33662184
Proper citation: RRID:Addgene_171216 Copy
Species: Homo sapiens
Genetic Insert: K-SPOTIT1.0
Vector Backbone Description: Backbone Size:6778; Vector Backbone:PLX208; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33662184
Proper citation: RRID:Addgene_171215 Copy
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