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Species: Homo sapiens
Genetic Insert: HDAC6.DC
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20133936
Comments: Additional Reference:
Gao etal "Histone Deacetylase 6 Regulates Growth Factor-Induced Actin Remodeling and Endocytosis" MOLECULAR AND CELLULAR BIOLOGY, Dec. 2007, p. 8637–8647
Proper citation: RRID:Addgene_36189 Copy
Species: Homo sapiens
Genetic Insert: HDAC6..delta Buz
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20133936
Proper citation: RRID:Addgene_36190 Copy
Species: Homo sapiens
Genetic Insert: H2A
Vector Backbone Description: Vector Backbone:pmTurquoise2-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22434194
Comments: Please note that there is a 3bp mismatch between Addgene's quality control sequence and the provided sequence from the depositing lab. This mismatch should not affect function or localization.
Proper citation: RRID:Addgene_36207 Copy
Species: Homo sapiens
Genetic Insert: Golgi targeting sequence
Vector Backbone Description: Vector Backbone:pmTurquoise2-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22434194
Proper citation: RRID:Addgene_36205 Copy
Species: Homo sapiens
Genetic Insert: Sec23A
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pLVX-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21045114
Comments: The cDNA sequence encoding mRuby (Kredel et al., 2009) underwent codon usage
adaptation before gene synthesis (MWG Eurofins) and cloning into pBluescript II
SK(+). mRuby was excised with SalI-EcoRI for insertion into pLVX-Puro (Clontech)
to generate pLVX-Puro–mRuby. A cDNA encoding human Sec23A was amplified
by PCR, gel purified and subcloned via pGEM-T vector (excised with ApaI-XbaI)
and cloned into pLVX-Puro–mRuby.
Proper citation: RRID:Addgene_36158 Copy
Species: Homo sapiens
Genetic Insert: Sec16A
Vector Backbone Description: Backbone Marker:Clontech, then modified; Vector Backbone:pVenus-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17005010
Proper citation: RRID:Addgene_36157 Copy
Species: Homo sapiens
Genetic Insert: Sec16A
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17005010
Proper citation: RRID:Addgene_36156 Copy
Species: Homo sapiens
Genetic Insert: p150Glued
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16772339
Proper citation: RRID:Addgene_36154 Copy
Species: Homo sapiens
Genetic Insert: MeCP2
Vector Backbone Description: Backbone Marker:Steve Elledge; Backbone Size:6699; Vector Backbone:pSMP; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388813
Proper citation: RRID:Addgene_36379 Copy
Species: Homo sapiens
Genetic Insert: MBD3
Vector Backbone Description: Backbone Marker:Steve Elledge; Backbone Size:6699; Vector Backbone:pSMP; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388813
Proper citation: RRID:Addgene_36372 Copy
Species: Homo sapiens
Genetic Insert: MBD3
Vector Backbone Description: Backbone Marker:Steve Elledge; Backbone Size:6699; Vector Backbone:pSMP; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388813
Proper citation: RRID:Addgene_36371 Copy
Species: Homo sapiens
Genetic Insert: MBD2
Vector Backbone Description: Backbone Marker:Steve Elledge; Backbone Size:6699; Vector Backbone:pSMP; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388813
Proper citation: RRID:Addgene_36369 Copy
Species: Homo sapiens
Genetic Insert: Crkl SH2 domain
Vector Backbone Description: Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7545163
Comments: Constructed by Johanna ten Hoeve.
Proper citation: RRID:Addgene_36402 Copy
Species: Homo sapiens
Genetic Insert: MBD2
Vector Backbone Description: Backbone Marker:Steve Elledge; Backbone Size:6699; Vector Backbone:pSMP; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388813
Proper citation: RRID:Addgene_36368 Copy
Species: Homo sapiens
Genetic Insert: MBD1
Vector Backbone Description: Backbone Marker:Steve Elledge; Backbone Size:6699; Vector Backbone:pSMP; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388813
Proper citation: RRID:Addgene_36367 Copy
Species: Homo sapiens
Genetic Insert: MBD1
Vector Backbone Description: Backbone Marker:Steve Elledge; Backbone Size:6699; Vector Backbone:pSMP; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388813
Proper citation: RRID:Addgene_36366 Copy
Species: Homo sapiens
Genetic Insert: Bcr encoding residues 1004-1271
Vector Backbone Description: Vector Backbone:pGEX 3X; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17116687
Comments: We subcloned a Pvu II-EcoRI fragment into pGEX-3X digested with Sma I -EcoRI. This includes the natural stop codon and 3' untranslated sequences. The construct encodes the GTPase activating domain of Bcr which is active against GTP-bound Rac. The Pvu II site is located in the amino acid string -QLDP-. Constructed by Vesa Kaartinen. The Rosetta2 strain may improve yield of the fusion protein.
Proper citation: RRID:Addgene_36405 Copy
Species: Homo sapiens
Genetic Insert: Crkl tandem SH3 domains
Vector Backbone Description: Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7545163
Comments: This construct includes the N- and C-terminal SH3 domains of Crkl, including residues 115-303. It is a 630 bp BsmI fragment (blunt) cloned into the SmaI site of pGEX-2T. Constructed by johanna ten Hoeve. Additional reference PMID 9053848.
Proper citation: RRID:Addgene_36403 Copy
Species: Homo sapiens
Genetic Insert: MBD1
Vector Backbone Description: Backbone Marker:Steve Elledge; Backbone Size:6699; Vector Backbone:pSMP; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388813
Proper citation: RRID:Addgene_36365 Copy
Species: Homo sapiens
Genetic Insert: NR2F1
Vector Backbone Description: Backbone Marker:Steve Elledge; Backbone Size:6699; Vector Backbone:pSMP; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388813
Proper citation: RRID:Addgene_36363 Copy
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