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Species: Synthetic
Genetic Insert: 4xLexAOp-O2
Vector Backbone Description: Backbone Size:2171; Vector Backbone:pBSK-O1; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34525356
Comments: Enhancer
Proper citation: RRID:Addgene_165816 Copy
Species: Synthetic
Genetic Insert: All-in-one FC31 RMCE Cassette
Vector Backbone Description: Backbone Size:2171; Vector Backbone:pColE1_Omega1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34525356
Comments: Omega1
Proper citation: RRID:Addgene_165887 Copy
Species: Synthetic
Genetic Insert: All-in-one FC31 RMCE Cassette
Vector Backbone Description: Backbone Size:2171; Vector Backbone:pColE1_Omega1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34525356
Comments: Omega1
Proper citation: RRID:Addgene_165888 Copy
Species: Synthetic
Genetic Insert: All-in-one FC31 RMCE Cassette
Vector Backbone Description: Backbone Size:2171; Vector Backbone:pColE1_Omega1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34525356
Comments: Omega1
Proper citation: RRID:Addgene_165889 Copy
Species: Pseudomonas putida GPo1
Genetic Insert: alkS
Vector Backbone Description: Vector Backbone:pCK01; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34767976
Proper citation: RRID:Addgene_166502 Copy
Species: Catharanthus roseus (Madagascar periwinkle)
Genetic Insert: 8-hydroxygeraniol oxidoreductase; 10-hydroxygeraniol oxidoreductase; alcohol dehydrogenase 10
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:36088516
Comments: Genbank accession: KF302069, the CDS sequence has been domesticated (all endogenous BpiI, BsaI, BsmBI and SapI recognition sites removed) and are flanked by an inverted pair of BsaI recognition sites that produce overhangs compatible with the phytobrick assembly standard
Proper citation: RRID:Addgene_177023 Copy
Species: Catharanthus roseus (Madagascar periwinkle)
Genetic Insert: iridoid oxidase; CYP76A26
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:36088516
Comments: Genbank accession: KF302066, amplified from cDNA, contains T359S relative to KF302066, the CDS sequence has been domesticated (all endogenous BpiI, BsaI, BsmBI and SapI recognition sites removed) and are flanked by an inverted pair of BsaI recognition sites that produce overhangs compatible with the phytobrick assembly standard
Proper citation: RRID:Addgene_177026 Copy
Species: Nepeta mussinii (aka Nepeta racemosa)
Genetic Insert: major latex protein-like
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:36088516
Comments: Genbank accession: MT108267.1, the CDS sequence has been domesticated (all endogenous BpiI, BsaI, BsmBI and SapI recognition sites removed) and are flanked by an inverted pair of BsaI recognition sites that produce overhangs compatible with the phytobrick assembly standard
Proper citation: RRID:Addgene_177025 Copy
Species: Catharanthus roseus (Madagascar periwinkle)
Genetic Insert: 7-deoxyloganetic acid glucosyl transferase; UGT709C2
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:36088516
Comments: Genbank accession: KF302068, the CDS sequence has been domesticated (all endogenous BpiI, BsaI, BsmBI and SapI recognition sites removed) and are flanked by an inverted pair of BsaI recognition sites that produce overhangs compatible with the phytobrick assembly standard
Proper citation: RRID:Addgene_177027 Copy
Species: Picea abies (Norway spruce)
Genetic Insert: geranyl pyrophosphate synthase; geranyl diphosphate synthase
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:36088516
Comments: Genbank accession: GQ369788, transit peptide removed, the CDS sequence has been domesticated (all endogenous BpiI, BsaI, BsmBI and SapI recognition sites removed) and are flanked by an inverted pair of BsaI recognition sites that produce overhangs compatible with the phytobrick assembly standard
Proper citation: RRID:Addgene_177020 Copy
Species: Catharanthus roseus (Madagascar periwinkle)
Genetic Insert: geraniol 8-oxidase; geraniol-10-hydroxylase; CYP76B6
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:36088516
Comments: Genbank accession: AJ251269, the CDS sequence has been domesticated (all endogenous BpiI, BsaI, BsmBI and SapI recognition sites removed) and are flanked by an inverted pair of BsaI recognition sites that produce overhangs compatible with the phytobrick assembly standard
Proper citation: RRID:Addgene_177022 Copy
Species: Catharanthus roseus (Madagascar periwinkle)
Genetic Insert: geraniol synthase
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:36088516
Comments: Genbank accession: JN882024, transit peptide removed, the CDS sequence has been domesticated (all endogenous BpiI, BsaI, BsmBI and SapI recognition sites removed) and are flanked by an inverted pair of BsaI recognition sites that produce overhangs compatible with the phytobrick assembly standard
Proper citation: RRID:Addgene_177021 Copy
Species: Streptomyces phage phiC31
Genetic Insert: phiC31 integrase plus SV40 nuclear localization sequence (PKKKRKV)
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:36088516
Comments: codon optimized for N. benthamiana expression, the CDS sequence has been domesticated (all endogenous BpiI, BsaI, BsmBI and SapI recognition sites removed) and are flanked by an inverted pair of BsaI recognition sites that produce overhangs compatible with the phytobrick assembly standard, derived from GB1496
Proper citation: RRID:Addgene_177035 Copy
Species: Other
Genetic Insert: 2x opattB binding site plus minimal CaMV 35S promoter plus partial TMV 'UTR
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:36088516
Comments: the CDS sequence has been domesticated (all endogenous BpiI, BsaI, BsmBI and SapI recognition sites removed) and are flanked by an inverted pair of BsaI recognition sites that produce overhangs compatible with the phytobrick assembly standard
Proper citation: RRID:Addgene_177037 Copy
Species: Catharanthus roseus (Madagascar periwinkle)
Genetic Insert: secologanin synthase; CYP72C
Vector Backbone Description: Vector Backbone:pTwist; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:36088516
Comments: Genbank accession: KF309242.1 or KF415117.1, the CDS sequence has been domesticated (all endogenous BpiI, BsaI, BsmBI and SapI recognition sites removed) and are flanked by an inverted pair of BsaI recognition sites that produce overhangs compatible with the phytobrick assembly standard
Proper citation: RRID:Addgene_177030 Copy
Species: E. coli
Genetic Insert: SoxS
Vector Backbone Description: Vector Backbone:p15A; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34800362
Proper citation: RRID:Addgene_177323 Copy
Species: L. mobilis
Genetic Insert: a gRNA that targets next to PAM library, used for L. mobilis type I-E system
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:35216669
Proper citation: RRID:Addgene_178752 Copy
Species: C. difficile / synthetic
Genetic Insert: CBD-SmBiT/LgBiT
Vector Backbone Description: Backbone Marker:W. K. Smits, Leiden University Medical Center; Vector Backbone:pAF256; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:35377223
Comments: Please also cite:
Oliveira Paiva et al J Mol Biol. 2019 Jan 8. pii: S0022-2836(18)31160-4. doi: 10.1016/j.jmb.2019.01.001.
Proper citation: RRID:Addgene_178596 Copy
Species: A. chroococcum
Genetic Insert: gRNA that targets next to PAM library, used for A. chroococcum type I-E #2 system
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:35216669
Proper citation: RRID:Addgene_178740 Copy
Species: A. chroococcum
Genetic Insert: gRNA that targets next to PAM library, used for A. chroococcum type I-E #3 system
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:35216669
Proper citation: RRID:Addgene_178746 Copy
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