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Species: Synthetic
Genetic Insert: Moth Pheromone pathway
Vector Backbone Description: Vector Backbone:pDGB3_omega1; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37032497
Comments: Contains the Transcriptional Units for constitutive expression of kanamycin resistance, and for dCasEV2.1-regulated expression of Atr delta11, HarFAR and SpATF1-2
Proper citation: RRID:Addgene_187813 Copy
Species: Hemophilus influenzae
Genetic Insert: HindII
Vector Backbone Description: Backbone Size:57000; Vector Backbone:pTA-Mob 2.0; Vector Types:Bacterial Expression, Yeast Expression, Synthetic Biology, Diatom Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:37850145
Proper citation: RRID:Addgene_188625 Copy
Species: Homo sapiens
Genetic Insert: FBW7
Vector Backbone Description: Vector Backbone:pDEST-Myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33824312
Proper citation: RRID:Addgene_197455 Copy
Species: Homo sapiens
Genetic Insert: Cas9-CtIP-dnRNF168
Vector Backbone Description: Backbone Size:3197; Vector Backbone:pPIX; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37070192
Proper citation: RRID:Addgene_200254 Copy
Species: E. coli
Genetic Insert: folA, thyA
Vector Backbone Description: Vector Backbone:pTET-duet; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38637543
Comments: Please visit https://doi.org/10.1101/2023.05.28.542639 for bioRxiv preprint.
Proper citation: RRID:Addgene_191293 Copy
Species: E. coli
Genetic Insert: folA, thyA
Vector Backbone Description: Vector Backbone:pTET-duet; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38637543
Comments: Please visit https://doi.org/10.1101/2023.05.28.542639 for bioRxiv preprint.
Proper citation: RRID:Addgene_191292 Copy
Species: Epstein-Barr virus
Genetic Insert: BLRF1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2875; Vector Backbone:pENTR223.1; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:17446270
Proper citation: RRID:Addgene_195771 Copy
Species: E. coli
Genetic Insert: folA, thyA
Vector Backbone Description: Vector Backbone:pTET-duet; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38637543
Comments: Please visit https://doi.org/10.1101/2023.05.28.542639 for bioRxiv preprint.
Proper citation: RRID:Addgene_191290 Copy
Species: E. coli
Genetic Insert: folA, thyA
Vector Backbone Description: Vector Backbone:pTET-duet; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38637543
Comments: Please visit https://doi.org/10.1101/2023.05.28.542639 for bioRxiv preprint.
Proper citation: RRID:Addgene_191297 Copy
Species: E. coli
Genetic Insert: folA, thyA
Vector Backbone Description: Vector Backbone:pTET-duet; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38637543
Comments: Please visit https://doi.org/10.1101/2023.05.28.542639 for bioRxiv preprint.
Proper citation: RRID:Addgene_191296 Copy
Species: Danio rerio
Genetic Insert: bmp7
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCS2; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19377468
Proper citation: RRID:Addgene_201909 Copy
Species: Homo sapiens
Genetic Insert: GB1-His6-TEV-CHMP4C(121-233, S191C, SSSEEE)
Vector Backbone Description: Backbone Size:5643; Vector Backbone:pET-11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_180616 Copy
Species: E. coli
Genetic Insert: folA, thyA
Vector Backbone Description: Vector Backbone:pTET-duet; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38637543
Comments: Please visit https://doi.org/10.1101/2023.05.28.542639 for bioRxiv preprint.
Proper citation: RRID:Addgene_191310 Copy
Species: Streptomyces rubellomurinus
Genetic Insert: FrbA
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37122473
Comments: The pCDF-Frb-v1 plasmid can be propagated reliably in DH10b cells, but it is good practice to minimize the number of propagations. Upon receiving cells and growing a colony overnight in liquid culture, make a frozen glycerol stock of these Dh10b cells containing plasmid. This glycerol stock can be used to inoculate future cultures for isolating more plasmid.
Glycerol stocks can be made by mixing 600 uL of overnight culture with 400 uL of 50% (v/v) sterile glycerol and placing tube in a -80C freezer.
This plasmid should be paired with pERM2-nhpSer (#201922, which enables translational encoding of the biosynthesized non-hydrolyzable phosphoserine amino acid) and a compatible plasmid expressing protein of interest (e.g. #174076 for a sfGFP-150TAG control) in the cell line BL21(DE3) deltaSerC (#197656). This system is used to translationally incorporate non-hydrolyzable phosphoserine into target proteins at TAG amber stop codons in E coli.
This plasmid exists as a dimer. This is not expected to impact the end function of the plasmid and the depositor recommends that the plasmid should be used exactly as provided. However, please refer to the Addgene Blog post on multimers for more information: https://blog.addgene.org/plasmids-101-dimers-and-multimers
Additionally, a small insertion in the CloDF13 ori sequence has been identified in one half of the plasmid dimer. The depositor has confirmed that this has not impacted plasmid function.
Proper citation: RRID:Addgene_201923 Copy
Species: Methanococcus maripaludis
Genetic Insert: SepRS(2)
Vector Backbone Description: Backbone Marker:N/A; Vector Backbone:Custom; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37122473
Comments: This plasmid should be paired with pCDF-Frb-v1 (#201923, which enables the biosynthesis of non-hydrolyzable phosphoserine) and a compatible plasmid expressing protein of interest (e.g. #174076 for a sfGFP-150TAG control) in the cell line BL21(DE3) deltaSerC (#197656). This system is used to translationally incorporate non-hydrolyzable phosphoserine into target proteins at TAG amber stop codons in E coli.
Proper citation: RRID:Addgene_201922 Copy
Species: HIV-1
Genetic Insert: HIV-1 3' end (HXB2: 9045 till poly-A(10)
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:3929; Vector Backbone:pCR®2.1-TOPO®; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37426753
Proper citation: RRID:Addgene_197894 Copy
Species: E. coli
Genetic Insert: folA, thyA
Vector Backbone Description: Vector Backbone:pTET-duet; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38637543
Comments: Please visit https://doi.org/10.1101/2023.05.28.542639 for bioRxiv preprint.
Proper citation: RRID:Addgene_191288 Copy
Species: E. coli
Genetic Insert: folA, thyA
Vector Backbone Description: Vector Backbone:pTET-duet; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38637543
Comments: Please visit https://doi.org/10.1101/2023.05.28.542639 for bioRxiv preprint.
Proper citation: RRID:Addgene_191287 Copy
Vector Backbone Description: Backbone Size:10183; Vector Backbone:plex_307; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_201137 Copy
Species: Synthetic
Genetic Insert: FRB-C2-dCer3
Vector Backbone Description: Vector Backbone:P_actin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36332023
Proper citation: RRID:Addgene_191327 Copy
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