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Species: Mus musculus
Genetic Insert: Arl13b
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7100; Vector Backbone:pcDNA-DEST40; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21976698
Comments: In Larkins et al (2011), this is plasmid "Arl13b untagged" as referenced in Figure 2. Full-length Arl13b with stop codon (pENTR-Arl13b1) was Gateway cloned into pcDNA-DEST40. Because of the stop codon, the backbone-encoded C-terminal tags are not expressed.
Proper citation: RRID:Addgene_40874 Copy
Species: Mus musculus
Genetic Insert: Arl13b
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7100; Vector Backbone:pcDNA-DEST40; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21976698
Comments: Full-length Arl13b without a stop codon (pENTR-Arl13b2) was Gateway cloned into pcDNA-DEST40.
Proper citation: RRID:Addgene_40873 Copy
Species: Mus musculus
Genetic Insert: TAZ
Vector Backbone Description: Vector Backbone:pGEX-4T-2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11118213
Comments: Fragments coding for the mTAZ WW domain (amino acids 122-164 and 79-164) were subcloned into pGEX4T2 (Amersham Pharmacia Biotech) for bacterial expression.
Proper citation: RRID:Addgene_40915 Copy
Species: Mus musculus
Genetic Insert: Arl13b
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7780; Vector Backbone:pcDNA-DEST47; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21976698
Comments: In Larkins et al (2011), this is plasmid "Arl13b GFP tagged" as referenced in Figure 2. Full-length Arl13b without a stop codon (pENTR-Arl13b2) was Gateway cloned into pcDNA-DEST47.
Proper citation: RRID:Addgene_40872 Copy
Species: Mus musculus
Genetic Insert: Arl13b
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2601; Vector Backbone:pENTR/SD/D-TOPO; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21976698
Proper citation: RRID:Addgene_40870 Copy
Species: Mus musculus
Genetic Insert: EPLIN beta
Vector Backbone Description: Vector Backbone:pCMV5-Flag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11179679
Proper citation: RRID:Addgene_40956 Copy
Species: Mus musculus
Genetic Insert: Ellis van Creveld syndrome 2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7500; Vector Backbone:pEF5/FRT/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22981989
Proper citation: RRID:Addgene_41009 Copy
Species: Mus musculus
Genetic Insert: Ellis van Creveld syndrome 2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7500; Vector Backbone:pEF5/FRT/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22981989
Proper citation: RRID:Addgene_41008 Copy
Species: Mus musculus
Genetic Insert: Ellis van Creveld syndrome 2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7500; Vector Backbone:pEF5/FRT/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22981989
Proper citation: RRID:Addgene_41007 Copy
Species: Mus musculus
Genetic Insert: Ellis van Creveld syndrome 2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7500; Vector Backbone:pEF5/FRT/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22981989
Proper citation: RRID:Addgene_41005 Copy
Species: Mus musculus
Genetic Insert: p160 myb binding protein
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1 myc-His A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14744933
Comments: There is a K935R mutation in this insert. The mutation is not known to affect p160MBP function.
Proper citation: RRID:Addgene_41 Copy
Species: Mus musculus
Genetic Insert: Ellis van Creveld syndrome 2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7500; Vector Backbone:pEF5/FRT/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22981989
Proper citation: RRID:Addgene_41004 Copy
Species: Mus musculus
Genetic Insert: shh
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: The ShhN construct contains a stop codon following the site of internal cleavage and therefore produces a protein (ShhN) containing the same amino acid residues as the processed protein but lacking cholesterol modification.
Proper citation: RRID:Addgene_37680 Copy
Species: Mus musculus
Genetic Insert: shh
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12372301
Comments: The ShhN construct contains a stop codon following the site of internal cleavage and therefore produces a protein (ShhN) containing the same amino acid residues as the processed protein but lacking cholesterol modification.
Renilla luciferase was inserted in-frame into the amino-terminal signaling domain located in a peripheral loop within the structure of ShhN.
Proper citation: RRID:Addgene_37677 Copy
Species: Mus musculus
Genetic Insert: Gli2
Vector Backbone Description: Vector Backbone:pCEFL; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19996169
Proper citation: RRID:Addgene_37671 Copy
Species: Mus musculus
Genetic Insert: Gli2
Vector Backbone Description: Vector Backbone:pCEFL; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19996169
Comments: monomeric GFP was constructed by introducing the monomeric mutation, A206K, to EGFP.
Proper citation: RRID:Addgene_37672 Copy
Species: Mus musculus
Genetic Insert: Smo
Vector Backbone Description: Vector Backbone:pEG; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10984056
Comments: Addgene NGS results identified a D54N variant compared to the NCBI reference [EDL13766.1].
Proper citation: RRID:Addgene_37674 Copy
Species: Mus musculus
Genetic Insert: Estrogen-related receptor gamma
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24684682
Proper citation: RRID:Addgene_37849 Copy
Species: Mus musculus
Genetic Insert: Clathrin, light polypeptide (LCa)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-N1; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21552254
Comments: Note: The clathrin light chain has an A9D amino acid residue substitution. This is a natural variant that does not affect protein function.
Proper citation: RRID:Addgene_38012 Copy
Species: Mus musculus
Genetic Insert: Clathrin, light polypeptide (LCa)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-N1; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21552254
Comments: Note: The clathrin light chain has an A9D amino acid residue substitution. This is a natural variant that does not affect protein function.
Proper citation: RRID:Addgene_38010 Copy
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