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Species: Saccharomyces cerevisiae
Genetic Insert: Sup35R1+8H1Q
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pRS306; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17893150
Proper citation: RRID:Addgene_17722 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sup35R1+4
Vector Backbone Description: Backbone Size:2300; Vector Backbone:pJC25; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17893150
Proper citation: RRID:Addgene_17723 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sup35R1+4
Vector Backbone Description: Backbone Size:2300; Vector Backbone:pJC25; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17893150
Proper citation: RRID:Addgene_17725 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA_RNH1_RNH201
Vector Backbone Description: Vector Backbone:p0054; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34107026
Proper citation: RRID:Addgene_177271 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:6300; Vector Backbone:pRS415U; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34107026
Proper citation: RRID:Addgene_177272 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ADH1t:T7p:cgRNA_HIS3_stop:tZ
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pEDJ400; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34107026
Proper citation: RRID:Addgene_177275 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ADH1t:HIS3_23∆29-XII-5 :<-PGK1p
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pCfB2909; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34107026
Comments: Integrative plasmid
Please note: Plasmid contains a 68bp insertion in mADH1t compared to the depositor's provided sequence. This insertion is not known to affect plasmid function.
Proper citation: RRID:Addgene_177274 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL80
Vector Backbone Description: Backbone Size:7855; Vector Backbone:pCaSpeR4; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10197526
Proper citation: RRID:Addgene_17748 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pTEFmut8-mCherry
Vector Backbone Description: Vector Backbone:Empty backbone; Vector Types:Yeast Expression, Used as donor DNA in genome integration after linearisation with NotI; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35069506
Proper citation: RRID:Addgene_177706 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pTEFmut8-mTurquoise2-RNA_2.6--tCYC1
Vector Backbone Description: Vector Backbone:Empty backbone; Vector Types:Yeast Expression, Used as donor DNA in genome integration after linearisation with NotI; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35069506
Proper citation: RRID:Addgene_177711 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast MYO2 promoter
Vector Backbone Description: Backbone Size:5726; Vector Backbone:pRS426; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27323850
Comments: Please refer to Roney et al. 2016 Sci Rep. "Improvement of the reverse tetracycline transactivator by single amino acid substitutions that reduce leaky target gene expression to undetectable levels" (doi: 10.1038/srep27697) for more information.
Proper citation: RRID:Addgene_177925 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast TDH3 promoter
Vector Backbone Description: Backbone Size:5726; Vector Backbone:pRS426; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27323850
Comments: Please refer to Roney et al. 2016 Sci Rep. "Improvement of the reverse tetracycline transactivator by single amino acid substitutions that reduce leaky target gene expression to undetectable levels" (doi: 10.1038/srep27697) for more information.
Proper citation: RRID:Addgene_177924 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase
Vector Backbone Description: Backbone Size:0; Vector Backbone:n/a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:12778056
Comments: does not contain lacI/
ERG1, ERG8, MVD1: S. cerevisiae/
idi: E. Coli
Proper citation: RRID:Addgene_17816 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MevT
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBAD24; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17239639
Proper citation: RRID:Addgene_17820 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SUB2
Vector Backbone Description: Backbone Size:6018; Vector Backbone:pRS315; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11156604
Proper citation: RRID:Addgene_111302 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PRP5
Vector Backbone Description: Backbone Size:7950; Vector Backbone:yCP50; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8722763
Comments: Full plasmid sequence contains several ambiguous bases in the backbone- Unsure if these regions will affect function.
Proper citation: RRID:Addgene_111303 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SNU114
Vector Backbone Description: Backbone Size:4887; Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15911574
Proper citation: RRID:Addgene_111308 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SNU114
Vector Backbone Description: Backbone Size:4783; Vector Backbone:pRS314; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15911574
Proper citation: RRID:Addgene_111309 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PRP8
Vector Backbone Description: Vector Backbone:pRS303; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8725222
Comments: Please note PRP8 has a G1801D mutation- unsure if this affects protein function.
Proper citation: RRID:Addgene_111306 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CWC25
Vector Backbone Description: Vector Backbone:pET28B; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_111340 Copy
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