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Species: Synthetic
Genetic Insert: BSD2A
Vector Backbone Description: Backbone Size:9123; Vector Backbone:pKLV2-U6(BsmBI)-EF1a-BFP-Nkx2-5; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_117136 Copy
Vector Backbone Description: Backbone Size:4555; Vector Backbone:NA; Vector Types:Mammalian Expression, Cre/Lox, Acceptor vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16519801
Comments: Acceptor vector for the Creator Splice system.
Proper citation: RRID:Addgene_11707 Copy
Species: Arabidopsis thaliana
Genetic Insert: CIBN
Vector Backbone Description: Backbone Marker:Genlantis; Backbone Size:4962; Vector Backbone:phCMV-NGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31582560
Comments: Please visit https://www.biorxiv.org/content/10.1101/551788v3 for bioRxiv preprint.
Proper citation: RRID:Addgene_117243 Copy
Species: Other
Genetic Insert: ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30217854
Proper citation: RRID:Addgene_117235 Copy
Species: Other
Genetic Insert: ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30217854
Proper citation: RRID:Addgene_117236 Copy
Species: Homo sapiens
Genetic Insert: Cry2531-mCh-BAXS184E
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4018; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31582560
Comments: Please visit https://www.biorxiv.org/content/10.1101/551788v3 for bioRxiv preprint.
Proper citation: RRID:Addgene_117238 Copy
Species: Streptoalloteichus hindustanus
Genetic Insert: shble
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Thraustochytrid expression; Bacterial Resistance:Ampicillin
Comments: cloning method is Infusion cloning (Clontech) and restriction digest
Proper citation: RRID:Addgene_117226 Copy
Species: Homo sapiens
Genetic Insert: SMAD2
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV5B; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8752209
Proper citation: RRID:Addgene_11734 Copy
Species: Homo sapiens
Genetic Insert: 3' UTR ASIC1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:7334; Vector Backbone:pmiRGLO; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30292704
Comments: 5' NheI cloning site destroyed, 3' XbaI cloning site destroyed
Proper citation: RRID:Addgene_117339 Copy
Species: Mus musculus
Genetic Insert: Egr-1
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15225550
Proper citation: RRID:Addgene_11729 Copy
Species: E. coli
Genetic Insert: b-lactamase
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:2642; Vector Backbone:modified pGPS3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11602576
Comments: Positive control for mutagenesis experiments: reversion of the stop codon results in ampicillin resistance.
The pGPS3 has a deletion from BglI to BglII that destroys the ampicillin resistance gene. The ampicillin resistance gene containing the stop codon was then cloned in between AflIII and KpnI.
Please also see pGFPuv from BD Biosciences as another control for forward mutation assays. Catalog # 6079-1 http://www.clontech.com/US/Products/Fluorescent_Proteins_and_Reporters/Fluorescent_Proteins_by_Name/GFP_GFPuv_Fluorescent_Proteins?sitex=10020:22372:US .
Proper citation: RRID:Addgene_11724 Copy
Species: Mus musculus
Genetic Insert: Sox9
Vector Backbone Description: Backbone Size:9063; Vector Backbone:Tet-O-FUW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30127505
Proper citation: RRID:Addgene_117269 Copy
Species: Mus musculus
Genetic Insert: Nfib
Vector Backbone Description: Backbone Size:10013; Vector Backbone:Tet-O-FUW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30127505
Proper citation: RRID:Addgene_117271 Copy
Species: Homo sapiens
Genetic Insert: DPP6
Vector Backbone Description: Backbone Marker:Genescript; Backbone Size:5400; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29378180
Proper citation: RRID:Addgene_117272 Copy
Species: Cas9 (Streptococcus pyogenes M1 GAS)
Genetic Insert: Cas9, promotor P11-guide-RNA, homologous arms of Lp_0537
Vector Backbone Description: Backbone Marker:Sheng Yang, Addgene Plasmid #62225; Backbone Size:4476; Vector Backbone:pCas; Vector Types:other; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30927506
Proper citation: RRID:Addgene_117259 Copy
Species: Lactobacillus plantarum WCSF1
Genetic Insert: PorfX-Lp_0640-42, sapK-sappR
Vector Backbone Description: Vector Backbone:pSIP411, pNZ5319; Vector Types:other; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30927506
Proper citation: RRID:Addgene_117261 Copy
Species: Synthetic
Genetic Insert: SauCas9 sgRNA targeting TLR 2.0
Vector Backbone Description: Backbone Marker:Addgene#50920; Vector Backbone:pLKO.1-puro-U6; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36070530
Comments: Plasmid contains an IS1 prokaryotic transposable element that does not affect plasmid function.
Please visit https://www.biorxiv.org/content/10.1101/864199v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_117409 Copy
Species: Synthetic
Genetic Insert: SpyCas9 sgRNA targeting TLR 2.0
Vector Backbone Description: Backbone Marker:Addgene#50920; Vector Backbone:pLKO.1-puro-U6; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36070530
Comments: Plasmid contains an IS1 prokaryotic transposable element that does not affect plasmid function.
Please visit https://www.biorxiv.org/content/10.1101/864199v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_117406 Copy
Species: DNA derived from yeast E. Coli, transposon Tn5, Corynebacterium Aequoria (jellyfish)
Genetic Insert: STOP-eGFP-ROSA26TV
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2900; Vector Backbone:pBSII KS; Vector Types:Cre/Lox, /STOP; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16782029
Comments: Targeting vector for cDNA knock-in into the Rosa26 locus.
See more information about the creation of this plasmid on the PDF attached to the CTV vector page http://www.addgene.org/15912
Proper citation: RRID:Addgene_11739 Copy
Species: Homo sapiens
Genetic Insert: SMAD3
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9702197
Proper citation: RRID:Addgene_11742 Copy
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