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Species: Saccharomyces cerevisiae
Genetic Insert: synthetic yeast Ubiquitin
Vector Backbone Description: Backbone Marker:Mike Ellison - University of Alberta; Vector Backbone:pES7; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9659916
Comments: BamHI-KpnI fragment from YEp96 (D. Ecker, U PENN) was cloned into pES7 (Mike Ellison, University of Alberta) cut with BamHI/KpnI.
2 µm S. cerevisiae origin
Proper citation: RRID:Addgene_32151 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: synthetic yeast Ubiquitin
Vector Backbone Description: Backbone Marker:Mike Ellison - University of Alberta; Vector Backbone:pTER62; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9659916
Comments: Ub-no Lys; CUP1-Ub K6R, K11R, K27R, K29R, K33A, K48R, K63R (all Lys Changed to Arg); BamHI-BglII UBI fragment from YEp96 (D. Ecker, U PENN) was cloned into pTER62 (Mike Ellison, University of Alberta) cut with BamHI and BglII.
2 µm S. cerevisiae origin
Proper citation: RRID:Addgene_32150 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: hsFlp1
Vector Backbone Description: Vector Backbone:pBDP; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21831835
Proper citation: RRID:Addgene_32148 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VPS8
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:5000; Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_32241 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: INP51
Vector Backbone Description: Backbone Marker:ATCC; Vector Backbone:PCplac22; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: HA tag is likely 3xHA
Proper citation: RRID:Addgene_32240 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: INP53
Vector Backbone Description: Backbone Marker:ATCC; Vector Backbone:YEplac181; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_32198 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VPS9
Vector Backbone Description: Vector Backbone:YCplac111; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12628920
Comments: To insert an HA epitope at the N-terminus of Vps9, a NotI site was introduced after the first methionine codon of VPS9 in pPS91 (Hama et al., 1999). NotI-VPS9 was then subcloned into the centromere-based vector YCplac111 and a fragment encoding an 3xHA tag flanked by NotI sites was inserted.
Proper citation: RRID:Addgene_32431 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 20XUAS
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2560; Vector Backbone:pDONR 221 P1-P4; Vector Types:Gateway MultiSite entry clone; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21931740
Comments: Please see http://www.everyvector.com/sequences/show_public/12858 for additional plasmid map.
Proper citation: RRID:Addgene_32307 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2557; Vector Backbone:pDONR 221 P5-P2; Vector Types:Gateway MultiSite entry clone; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21931740
Comments: Please see http://www.everyvector.com/sequences/show_public/12863 for additional plasmid map.
Proper citation: RRID:Addgene_32304 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ENT2
Vector Backbone Description: Backbone Marker:ATCC; Vector Backbone:YCplac22; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_32429 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: UBC4
Vector Backbone Description: Backbone Marker:ATCC; Vector Backbone:YCplac22; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_32440 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: LEU2MX6
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24215641
Proper citation: RRID:Addgene_33138 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RP51A-synthetic minimal intron
Vector Backbone Description: Vector Backbone:pLGSD5; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2655924
Comments: The PLint sequence was derived from the short version (delta2) of the RP51A intron (Pikielny et al., 1983). The basic strategy was to make a short intron of about 60 nucleotides, keeping as much as possible of the sequence near the consensus sequences, and adding convenient restriction sites.
Inserted sequence: GTATGTTAATATGGTTAACGTCGACCGTGTTTTTGATATCTATACTAACAGGCCTTTTAATAG
Proper citation: RRID:Addgene_33145 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Rad51
Vector Backbone Description: Backbone Size:5857; Vector Backbone:pYES2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20864034
Proper citation: RRID:Addgene_33231 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TRP1
Vector Backbone Description: Backbone Size:2300; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9717241
Proper citation: RRID:Addgene_41603 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TRP1
Vector Backbone Description: Backbone Size:2300; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9717241
Proper citation: RRID:Addgene_41601 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL1 promoter
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9717241
Proper citation: RRID:Addgene_41608 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL1 promoter
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9717241
Proper citation: RRID:Addgene_41607 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ura3-FUS1-UBIMdkGFP*-SpHis5-Tim9
Vector Backbone Description: Vector Backbone:pUC118; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23172645
Proper citation: RRID:Addgene_41563 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL1 promoter
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9717241
Proper citation: RRID:Addgene_41616 Copy
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