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Species: Saccharomyces cerevisiae
Genetic Insert: Part of URA3 5' UTR and yEVenus with stop codon for recombination with pr-yEVenus-PEST-ADH1t:URA
Vector Backbone Description: Vector Backbone:pRS410; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37369667
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.08.16.253310v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_164849 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HIS3
Vector Backbone Description: Backbone Size:6614; Vector Backbone:pCENBEVY-U; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24357599
Proper citation: RRID:Addgene_38229 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HIS3
Vector Backbone Description: Backbone Size:6614; Vector Backbone:pCENBEVY-U; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24357599
Proper citation: RRID:Addgene_38227 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Renilla Luciferase
Vector Backbone Description: Backbone Size:7122; Vector Backbone:pCENBEVY-U; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24357599
Proper citation: RRID:Addgene_38221 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gas1
Vector Backbone Description: Backbone Marker:Addgene 14147; Backbone Size:5173; Vector Backbone:pAG416Gal-ccdB; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22724584
Comments: The primers for the genomic PCR and the location of the HA tag are from Fujita, MBC, 17,834,2006.
Proper citation: RRID:Addgene_38313 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: LST8
Vector Backbone Description: Backbone Size:6100; Vector Backbone:YCplac111; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16002396
Comments: Expresses LST8 from LST8 promoter. Cloned as 1.7-kb SacI-PstI fragment into YCplac111. CYC1 terminator (CEN, LEU2)
Proper citation: RRID:Addgene_39211 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: KOG1
Vector Backbone Description: Backbone Size:6100; Vector Backbone:YCplac111; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12408816
Comments: Expresses 2x-myc-tagged KOG1 under control of KOG1 promoter. The two myc-tags are integrated between codons 1 and 2 of KOG1.
A 6.0 kb PCR fragment containing the KOG1 locus (−972 to +304 with respect to the ORF) was cloned into the XbaI and Asp718I sites of YCplac111; the sequence ATG ATC CCC GGG TTA ATT AAC GGT GAA CAA AAG CTA ATC TCC GAG GAA GAC TTG AAC GGT GAA CAA AAA TTA ATC TCA GAA GAA GAC TTG AAC GGA CTC GAC GGT GAA CAA encoding two myc tags was integrated between codons 1 and 2 of KOG1
Addgene's sequencing result identified a L1512P mutation in the KOG1 sequence when compared to GenBank reference sequence NP_012056.1. The depositing laboratory confirmed the plasmid is functional as described in the associated publication.
Proper citation: RRID:Addgene_39209 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TOR2
Vector Backbone Description: Backbone Size:7800; Vector Backbone:pSEYC68; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10973982
Comments: N-terminally HA-tagged TOR2 expressed under control of GAL promoter.
Proper citation: RRID:Addgene_39207 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: yEVenus-PEST
Vector Backbone Description: Backbone Size:6682; Vector Backbone:p405; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25232010
Proper citation: RRID:Addgene_60161 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: yEVenus-PEST
Vector Backbone Description: Backbone Size:6682; Vector Backbone:p405; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25232010
Proper citation: RRID:Addgene_60160 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RdLuc
Vector Backbone Description: Backbone Marker:DNA 2.0; Backbone Size:2707; Vector Backbone:pJ204; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25232010
Proper citation: RRID:Addgene_60117 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: promoter
Vector Backbone Description: Backbone Size:6000; Vector Backbone:p416; Vector Types:Yeast Expression, Synthetic Biology, Yeast synthetic hybrid promoter construct; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22565375
Proper citation: RRID:Addgene_60326 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TUB2
Vector Backbone Description: Vector Backbone:pRS424; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24633327
Comments: C-terminal tail is from TUBB1 (tubulin, beta 1 class VI) according to HUGO nomenclature: http://www.genenames.org/genefamilies/TUB
Proper citation: RRID:Addgene_60400 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TUB2
Vector Backbone Description: Vector Backbone:pRS424; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24633327
Proper citation: RRID:Addgene_60405 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TUB2
Vector Backbone Description: Vector Backbone:pRS424; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24633327
Proper citation: RRID:Addgene_60404 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TUB2
Vector Backbone Description: Vector Backbone:pRS424; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24633327
Proper citation: RRID:Addgene_60402 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TUB2
Vector Backbone Description: Vector Backbone:pRS424; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24633327
Proper citation: RRID:Addgene_60388 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TUB1
Vector Backbone Description: Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24633327
Proper citation: RRID:Addgene_60387 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TUB1
Vector Backbone Description: Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24633327
Comments: Johnson V, Ayaz P, Huddleston P, Rice LM.
Design, overexpression, and purification of polymerization-blocked yeast αβ-tubulin mutants.
Biochemistry. 2011 Oct 11;50(40):8636-44. doi: 10.1021/bi2005174. Epub 2011 Sep 16.
Proper citation: RRID:Addgene_60385 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TUB1
Vector Backbone Description: Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24633327
Proper citation: RRID:Addgene_60393 Copy
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