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Species: Synthetic
Genetic Insert: c-roGFP2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4010; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21372177
Proper citation: RRID:Addgene_125584 Copy
Species: Synthetic
Genetic Insert: dLight1.3b
Vector Backbone Description: Backbone Size:5020; Vector Backbone:pAAV-CAG; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32895537
Comments: Addgene NGS identified chicken beta-actin promoter, but not the CMV enhancer and chimeric intron often associated with CAG promoters.
Proper citation: RRID:Addgene_125707 Copy
Species: Synthetic
Genetic Insert: RdLight1
Vector Backbone Description: Backbone Size:4579; Vector Backbone:pAAV-hSyn; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32895537
Proper citation: RRID:Addgene_125708 Copy
Species: Synthetic
Genetic Insert: YdLight1
Vector Backbone Description: Backbone Size:3983; Vector Backbone:pEGFP_N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32895537
Proper citation: RRID:Addgene_125705 Copy
Species: Synthetic
Genetic Insert: GFP1–10
Vector Backbone Description: Backbone Marker:Tzu-Yin Liu; Vector Backbone:UBQ10:sXVE:GFP1–9; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33175385
Proper citation: RRID:Addgene_125663 Copy
Species: Synthetic
Genetic Insert: ymNeongreen-T2A-mCherry
Vector Backbone Description: Vector Backbone:pDRF1-GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30783202
Proper citation: RRID:Addgene_125700 Copy
Species: Synthetic
Genetic Insert: S11-GFP1–9
Vector Backbone Description: Backbone Marker:Tzu-Yin Liu; Vector Backbone:UBQ10:sXVE:GFP1–9; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33175385
Proper citation: RRID:Addgene_125665 Copy
Species: Synthetic
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:6799; Vector Backbone:LentiV_Blast; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31697837
Comments: Please visit https://www.biorxiv.org/content/10.1101/636969v1 for BioRxiv preprint
Proper citation: RRID:Addgene_125592 Copy
Species: Synthetic
Genetic Insert: sfGFP
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32183906
Comments: Similar to the situation with some other plasmids in this collection, the missing four nucleotides at the 5' end of the WPRE renders it unrecognizable to SnapGene, but the expression vector is functional.
Nevertheless, for sfGFP expression, the depositor recommends the updated versions of this plasmid, PB CAG-sfGFP (https://www.addgene.org/133568/) or PB-CA sfGFP (https://www.addgene.org/159094/), that can also be used without the piggyBac transposase.
Red fluorescent protein versions are also available: PB CAG-tdTomato (https://www.addgene.org/133569/) or PB-CA tdTomato (https://www.addgene.org/159095/).
Proper citation: RRID:Addgene_125747 Copy
Species: Synthetic
Genetic Insert: sfGFP-GSAx9-iCre-ERT2
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32183906
Comments: Linker between sfGFP and iCre-ERT2 is a clean GSAx9 sequence, similar in length to the mutated P2A sequence in the plasmid pCAG-sfGFP-iCre-ERT2 (#125749).
The sfGFP fluorescence from the sfGFP-iCre-ERT2 is dim, as described in Nam and Capecchi, 2020 (https://doi.org/10.1186/s13064-020-00139-5) and a Figshare page (https://doi.org/10.6084/m9.figshare.13218437.v35). In short, it is not visible to the eyes, but can be detected with flow cytometry.
Similar to the situation with some other plasmids in this collection, the missing four nucleotides at the 5' end of the WPRE renders it unrecognizable to SnapGene, but the expression vector is functional.
Proper citation: RRID:Addgene_125748 Copy
Species: Synthetic
Genetic Insert: SYK biosensor
Vector Backbone Description: Backbone Marker:Invitrogen, modified at Dr. Roger Tsien Lab in University of California, San Diego; Backbone Size:5505; Vector Backbone:pcDNA3.1’; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25541586
Proper citation: RRID:Addgene_125729 Copy
Species: Synthetic
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:pLX_TRC209; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_125715 Copy
Species: Synthetic
Genetic Insert: nScCas9-ABE7.10, wheat/human codon optimized
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_125820 Copy
Species: Synthetic
Genetic Insert: A3A-PBE, wheat codon optimized
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_125818 Copy
Species: Synthetic
Genetic Insert: nSpCas9-ABE7.10 base changer, human codon optimized
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_125810 Copy
Species: Synthetic
Genetic Insert: FnCas12a, human codon optimized
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_125811 Copy
Species: Synthetic
Genetic Insert: LbCas12a, human codon optimized
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_125812 Copy
Species: Synthetic
Genetic Insert: SpCas9-NG, human codon optimized
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_125809 Copy
Species: Synthetic
Genetic Insert: Endlinker
Vector Backbone Description: Backbone Marker:GenScript; Vector Backbone:pUC57-Kan-mini; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_125761 Copy
Species: Synthetic
Genetic Insert: Endlinker
Vector Backbone Description: Backbone Marker:GenScript; Vector Backbone:pUC57-Kan-mini; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_125762 Copy
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