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Species: Saccharomyces cerevisiae
Genetic Insert: GAL1 promoter
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9717241
Proper citation: RRID:Addgene_41615 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL1 promoter
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9717241
Proper citation: RRID:Addgene_41613 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL1 promoter
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9717241
Proper citation: RRID:Addgene_41612 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL1 promoter
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9717241
Proper citation: RRID:Addgene_41610 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SEC63-mRFP
Vector Backbone Description: Vector Backbone:pRS425; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18812321
Comments: Please note that Addgene's quality control sequence shows several discrepancies with depositor's reference sequence. These are in vector region and should not affect function.
Proper citation: RRID:Addgene_41837 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VPH2
Vector Backbone Description: Backbone Marker:Dan Gottschling (Addgene plasmid# 41894); Backbone Size:7882; Vector Backbone:pAG306-GPD-ccdB chr I; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23172144
Proper citation: RRID:Addgene_41897 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VMA1
Vector Backbone Description: Backbone Marker:Dan Gottschling (Addgene plasmid# 41894); Backbone Size:7882; Vector Backbone:pAG306-GPD-ccdB chr I; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23172144
Proper citation: RRID:Addgene_41896 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Mediator of the Replication Checkpoint
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:6021; Vector Backbone:pRS415; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12865299
Comments: There is a D304N in LEU, relative to LEU start aa, this is not thought to effect function.
Proper citation: RRID:Addgene_41923 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Atg18
Vector Backbone Description: Backbone Size:5756; Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22704557
Comments: Plasmid was constructed by amplifying Atg18 with 300bp 5' of the start site.
Proper citation: RRID:Addgene_42529 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Tpt1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDEST17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20075163
Comments: See publication for expression protocol. Expressed in BL21 (DE3) RIPLE cells from Stratagene. Highly expressed and soluble.
Proper citation: RRID:Addgene_42571 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HIS3
Vector Backbone Description: Backbone Size:6614; Vector Backbone:pCENBEVY-U; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24357599
Proper citation: RRID:Addgene_38229 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HIS3
Vector Backbone Description: Backbone Size:6614; Vector Backbone:pCENBEVY-U; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24357599
Proper citation: RRID:Addgene_38227 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Renilla Luciferase
Vector Backbone Description: Backbone Size:7122; Vector Backbone:pCENBEVY-U; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24357599
Proper citation: RRID:Addgene_38221 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gas1
Vector Backbone Description: Backbone Marker:Addgene 14147; Backbone Size:5173; Vector Backbone:pAG416Gal-ccdB; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22724584
Comments: The primers for the genomic PCR and the location of the HA tag are from Fujita, MBC, 17,834,2006.
Proper citation: RRID:Addgene_38313 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: LST8
Vector Backbone Description: Backbone Size:6100; Vector Backbone:YCplac111; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16002396
Comments: Expresses LST8 from LST8 promoter. Cloned as 1.7-kb SacI-PstI fragment into YCplac111. CYC1 terminator (CEN, LEU2)
Proper citation: RRID:Addgene_39211 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: KOG1
Vector Backbone Description: Backbone Size:6100; Vector Backbone:YCplac111; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12408816
Comments: Expresses 2x-myc-tagged KOG1 under control of KOG1 promoter. The two myc-tags are integrated between codons 1 and 2 of KOG1.
A 6.0 kb PCR fragment containing the KOG1 locus (−972 to +304 with respect to the ORF) was cloned into the XbaI and Asp718I sites of YCplac111; the sequence ATG ATC CCC GGG TTA ATT AAC GGT GAA CAA AAG CTA ATC TCC GAG GAA GAC TTG AAC GGT GAA CAA AAA TTA ATC TCA GAA GAA GAC TTG AAC GGA CTC GAC GGT GAA CAA encoding two myc tags was integrated between codons 1 and 2 of KOG1
Addgene's sequencing result identified a L1512P mutation in the KOG1 sequence when compared to GenBank reference sequence NP_012056.1. The depositing laboratory confirmed the plasmid is functional as described in the associated publication.
Proper citation: RRID:Addgene_39209 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TOR2
Vector Backbone Description: Backbone Size:7800; Vector Backbone:pSEYC68; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10973982
Comments: N-terminally HA-tagged TOR2 expressed under control of GAL promoter.
Proper citation: RRID:Addgene_39207 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MAG1-sctetR
Vector Backbone Description: Backbone Size:5500; Vector Backbone:PRS415; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23470991
Comments: Please note that Addgene's sequencing results identified a few nucleotide differences when compared to the full plasmid sequence provided by the depositing laboratory. According to the depositing lab, these differences are not a concern for the function of the plasmid.
Proper citation: RRID:Addgene_44752 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: yeast Vps23-UEV(1-160)
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pGST-parallel.2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21505419
Proper citation: RRID:Addgene_45924 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL4
Vector Backbone Description: Vector Backbone:pattB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25581800
Comments: Please see this additional reference https://www.ncbi.nlm.nih.gov/pubmed/20434990 for more information on the Q-system.
Proper citation: RRID:Addgene_46107 Copy
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