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Species: Synthetic
Genetic Insert: FKBP-mCherry
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4713; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33890764
Proper citation: RRID:Addgene_178863 Copy
Species: Synthetic
Genetic Insert: mCherry-FKBP-TEVprotease(Δ220-242)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4675; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33890764
Proper citation: RRID:Addgene_178867 Copy
Species: Synthetic
Genetic Insert: FKBP-mTagBFP2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3983; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33890764
Proper citation: RRID:Addgene_178864 Copy
Species: S. thermophilus
Genetic Insert: S. thermophilus type I-E cas5
Vector Backbone Description: Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35216669
Proper citation: RRID:Addgene_178778 Copy
Species: S. thermophilus
Genetic Insert: S. thermophilus type I-E cas6
Vector Backbone Description: Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35216669
Proper citation: RRID:Addgene_178779 Copy
Species: S. thermophilus
Genetic Insert: S. thermophilus type I-E cse2
Vector Backbone Description: Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35216669
Proper citation: RRID:Addgene_178777 Copy
Species: X. albilineans
Genetic Insert: X. albilineans type I-F1 cas2-3
Vector Backbone Description: Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35216669
Proper citation: RRID:Addgene_178769 Copy
Species: Synthetic
Genetic Insert: U6-Nla repeat-BbsI-Nla repeat
Vector Backbone Description: Backbone Marker:Lucigen; Backbone Size:1812; Vector Backbone:pSmart HC Kan; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35051351
Proper citation: RRID:Addgene_178883 Copy
Species: Synthetic
Genetic Insert: mScarlet counter-selection cassette
Vector Backbone Description: Backbone Size:2654; Vector Backbone:BASIC_SEVA_29.10; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36381610
Comments: Cloning method: BASIC DNA assembly . Please visit https://www.biorxiv.org/content/10.1101/2022.01.06.446575v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_178921 Copy
Species: X. albilineans
Genetic Insert: X. albilineans type I-C cas3
Vector Backbone Description: Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35216669
Proper citation: RRID:Addgene_178766 Copy
Species: Synthetic
Genetic Insert: mScarlet counter-selection cassette
Vector Backbone Description: Backbone Size:2729; Vector Backbone:BASIC_SEVA_27.10; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36381610
Comments: Cloning method: BASIC DNA assembly . Please visit https://www.biorxiv.org/content/10.1101/2022.01.06.446575v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_178920 Copy
Species: Synthetic
Genetic Insert: EF1a-NlaCas8c-bGH PolyA
Vector Backbone Description: Backbone Marker:Lucigen; Backbone Size:1993; Vector Backbone:pSmart HC Kan; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35051351
Proper citation: RRID:Addgene_178880 Copy
Genetic Insert: EF1a-NlaCas3c-bGH polyA
Vector Backbone Description: Backbone Marker:Lucigen; Backbone Size:1993; Vector Backbone:pSmart HC Kan; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35051351
Proper citation: RRID:Addgene_178881 Copy
Species: S. thermophilus
Genetic Insert: S. thermophilus type I-E cas7
Vector Backbone Description: Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35216669
Proper citation: RRID:Addgene_178780 Copy
Genetic Insert: Cargo EGFP with AttP Bxb1 site for in-frame fusion
Vector Backbone Description: Vector Backbone:pMC.BESPX; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36424489
Comments: Please visit https://doi.org/10.1101/2021.11.01.466786 for bioRxiv preprint.
Proper citation: RRID:Addgene_179116 Copy
Species: Homo sapiens
Genetic Insert: Src, SsrA
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34637702
Proper citation: RRID:Addgene_179141 Copy
Species: Haemophilus influenzae
Genetic Insert: SspB
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34637702
Proper citation: RRID:Addgene_179142 Copy
Species: Synthetic
Genetic Insert: Sec61b-V5-TurboID
Vector Backbone Description: Backbone Marker:Tong-Chuan He laboratory; Backbone Size:35725; Vector Backbone:pAdTrack-CMV, pAdEasy-1; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34471136
Comments: Sec61b-V5-TurboID_pcDNA5 is Addgene plasmid # 166971.
To generate adenovirus, digest with PacI and perform transfection to HEK293 cells according to Luo et al (PMID:17546019) procedure #14.
Please note: The plasmid contains a 1650bp insertion just downstream of the Left Arm compared to the depositor's provided sequence. This difference is not known to affect plasmid function.
Proper citation: RRID:Addgene_179143 Copy
Species: Mus musculus
Genetic Insert: kappa opioid receptor
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34162205
Proper citation: RRID:Addgene_179285 Copy
Species: Homo sapiens
Genetic Insert: ELMSAN1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5367; Vector Backbone:pET28C (+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34912035
Comments: The insert encodes the epitope (ELMSAN1 residue 550-600) of the antibody (Catalog # A303-157A, BETHYL). Expressed protein has been confirmed by Western blot.
Proper citation: RRID:Addgene_179391 Copy
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