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Species: Synthetic
Genetic Insert: mScarlet-F-gB-T2A-PuroR
Vector Backbone Description: Backbone Marker:Based on plasmids published by Schmid-Burgk et al. 2016 (DOI: 10.1038/ncomms12338); Backbone Size:2543; Vector Backbone:pCRISPaint; Vector Types:Gene tagging; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32750334
Proper citation: RRID:Addgene_171812 Copy
Species: Synthetic
Genetic Insert: sfGFP-SpyTag003 Loop B
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34324879
Proper citation: RRID:Addgene_171777 Copy
Species: Synthetic
Genetic Insert: sfGFP-SpyTag003 Loop A
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34324879
Proper citation: RRID:Addgene_171776 Copy
Species: Synthetic
Genetic Insert: mScarlet-F-Ova-T2A-BlastR
Vector Backbone Description: Backbone Marker:Based on plasmids published by Schmid-Burgk et al. 2016 (DOI: 10.1038/ncomms12338); Backbone Size:2577; Vector Backbone:pCRISPaint; Vector Types:Gene tagging; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32750334
Proper citation: RRID:Addgene_171817 Copy
Species: Synthetic
Genetic Insert: DogCatcher
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:3641; Vector Backbone:pDEST14; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34324879
Proper citation: RRID:Addgene_171772 Copy
Species: Synthetic
Genetic Insert: Promoter SPLc17 and RBSc21
Vector Backbone Description: Vector Backbone:pStA0; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34554258
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.11.983833v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171909 Copy
Species: Synthetic
Genetic Insert: Promoter SPLc45 and RBSc249
Vector Backbone Description: Vector Backbone:pStA0; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34554258
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.11.983833v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171906 Copy
Species: Synthetic
Genetic Insert: Promoter SPLc25 and RBSc249
Vector Backbone Description: Vector Backbone:pStA0; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34554258
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.11.983833v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171905 Copy
Species: Synthetic
Genetic Insert: RNA-based (p)ppGpp sensor
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5267; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34487413
Comments: Addgene QC identified a C66Y change in the lacI coding sequence that may impact the leakiness of expression of the (p)ppGpp sensor and NirFP insert.
Proper citation: RRID:Addgene_171921 Copy
Species: Synthetic
Genetic Insert: Promoter SPLc45 and RBSc21
Vector Backbone Description: Vector Backbone:pStA0; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34554258
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.11.983833v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171887 Copy
Species: Synthetic
Genetic Insert: RNA-based (p)ppGpp sensor
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5213; Vector Backbone:pET-28c; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34487413
Proper citation: RRID:Addgene_171920 Copy
Species: Synthetic
Genetic Insert: Promoter SPLc25 and RBSc21
Vector Backbone Description: Vector Backbone:pStA0; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34554258
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.11.983833v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171886 Copy
Species: Synthetic
Genetic Insert: Promoter SPLc3 and RBSc21
Vector Backbone Description: Vector Backbone:pStA0; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34554258
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.11.983833v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171884 Copy
Species: Synthetic
Genetic Insert: Cys-DogCatcher
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:3641; Vector Backbone:pDEST14; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34324879
Proper citation: RRID:Addgene_171929 Copy
Species: Synthetic
Genetic Insert: Promoter SPLc17 and RBSc123
Vector Backbone Description: Vector Backbone:pStA0; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34554258
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.11.983833v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171912 Copy
Species: Synthetic
Genetic Insert: Promoter SPLc17 and RBSc48
Vector Backbone Description: Vector Backbone:pStA0; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34554258
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.11.983833v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171910 Copy
Species: Synthetic
Genetic Insert: CbH-Cas9-RT-T2A-GFP, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
Vector Backbone Description: Vector Backbone:pCMV-PE2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34534334
Comments: Full sequence https://benchling.com/s/seq-hFIfAYFnD7vtemxXRY19.
Proper citation: RRID:Addgene_171994 Copy
Species: Synthetic
Genetic Insert: CbH-Cas9-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
Vector Backbone Description: Vector Backbone:pCMV-PE2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34534334
Comments: Full sequence https://benchling.com/s/seq-zQAEVlUC4JpjV6Gt4RFT.
Proper citation: RRID:Addgene_171992 Copy
Species: Synthetic
Genetic Insert: CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
Vector Backbone Description: Vector Backbone:pCMV-PE2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34534334
Comments: Full sequence: https://benchling.com/s/seq-btUItL879sEOzAzXhUuy.
Proper citation: RRID:Addgene_171991 Copy
Species: Synthetic
Genetic Insert: Promoter SPLc19 and RBSc123
Vector Backbone Description: Vector Backbone:pStA0; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34554258
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.11.983833v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171899 Copy
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