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Species: Streptococcus pyogenes serotype M1
Genetic Insert: SP-CAS9
Vector Backbone Description: Backbone Size:5562; Vector Backbone:pET-15; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25910214
Proper citation: RRID:Addgene_62731 Copy
Species: Synthetic
Genetic Insert: CRISPR-SP-Cas9 target seq + tdTomato (out of frame)
Vector Backbone Description: Backbone Size:8172; Vector Backbone:PHAGE2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25910214
Proper citation: RRID:Addgene_62733 Copy
Species: Camellia sinensis
Genetic Insert: flavaone 3B-hydroxylase
Vector Backbone Description: Backbone Size:5203; Vector Backbone:pETM6; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25527438
Comments: All genes were cloned in monocistronic form with T7 promoter and T7 terminator.
Proper citation: RRID:Addgene_62617 Copy
Species: Camellia sinensis
Genetic Insert: flavaone 3B-hydroxylase
Vector Backbone Description: Backbone Size:5203; Vector Backbone:pETM6; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25527438
Comments: All genes were cloned in monocistronic form with T7 promoter and T7 terminator.
Proper citation: RRID:Addgene_62618 Copy
Species: Camellia sinensis
Genetic Insert: flavaone 3B-hydroxylase
Vector Backbone Description: Backbone Size:5203; Vector Backbone:pETM6; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25527438
Comments: All genes were cloned in monocistronic form with T7 promoter and T7 terminator.
Proper citation: RRID:Addgene_62619 Copy
Species: Homo sapiens
Genetic Insert: CACNA1B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5100; Vector Backbone:pcDNA6/V5-HisA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25296916
Comments: contains alternate exons (+e10a, +18a, Δ19a, +e31a, +e37b and +e46)
Note that the human CaV2.2 insert contains the native stop codon. The V5 and His tags present in the pcDNA6/V5-HisA backbone are NOT in frame with CaV2.2.
Proper citation: RRID:Addgene_62574 Copy
Genetic Insert: amiR-eGFP123/amiR-eGFP419/tdTomato
Vector Backbone Description: Vector Backbone:pUC119; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26285215
Proper citation: RRID:Addgene_62690 Copy
Species: Homo sapiens
Genetic Insert: hAlpha7
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5600; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25056953
Proper citation: RRID:Addgene_62629 Copy
Species: Homo sapiens
Genetic Insert: SPRED1
Vector Backbone Description: Backbone Marker:Ambion; Backbone Size:6470; Vector Backbone:pMIR-Report; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25202123
Comments: There are a few mismatches outside of the mutated seed sequence; these mismatches should not affect plasmid function.
Proper citation: RRID:Addgene_62580 Copy
Species: Homo sapiens
Genetic Insert: SPRED1
Vector Backbone Description: Backbone Marker:Ambion; Backbone Size:6470; Vector Backbone:pMIR-Report; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25202123
Comments: There are a few mismatches outside of the mutated seed sequence; these mismatches should not affect plasmid function.
Proper citation: RRID:Addgene_62581 Copy
Species: Homo sapiens
Genetic Insert: CHRFAM7A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5600; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25056953
Proper citation: RRID:Addgene_62638 Copy
Species: Homo sapiens
Genetic Insert: Cerulean-P2A-PuroR
Vector Backbone Description: Backbone Size:3671; Vector Backbone:pUC57; Vector Types:Mammalian Expression, TALEN, homology-directed repair donor; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25765640
Comments: This plasmid contains the Cerulean fluorescent protein and loxP sites.
Proper citation: RRID:Addgene_62596 Copy
Vector Backbone Description: Backbone Size:6840; Vector Backbone:JDS70; Vector Types:Mammalian Expression, TALEN, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25765640
Proper citation: RRID:Addgene_62590 Copy
Vector Backbone Description: Backbone Size:6840; Vector Backbone:JDS78; Vector Types:Mammalian Expression, TALEN, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25765640
Proper citation: RRID:Addgene_62593 Copy
Species: Synthetic
Genetic Insert: OmpA_H1346
Vector Backbone Description: Backbone Marker:DNA2.0; Backbone Size:4000; Vector Backbone:pJexpress414; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26199411
Comments: Amino acid sequence: mkktaiaiavalagfatvaqaapndntWyTgAkLgWsqyhdtgfinnngpthenqQgIgAfAgIqVnpyVgFeMgYdWlgrmpykgsvengaykaqgVqLtAkLgYpitddVdVyLrLgImVwradtksnvygknhdtgvsPvFaGgVeYaItpeIaMrLeTqHtnnigdYPYDVPDYAtrpdnWmVsLgAsYrfgCCPGCCHHHHHH
Proper citation: RRID:Addgene_62647 Copy
Species: Synthetic
Genetic Insert: OmpA_H1256
Vector Backbone Description: Backbone Marker:DNA2.0; Backbone Size:4000; Vector Backbone:pJexpress414; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26199411
Comments: Amino acid sequence: mkktaiaiavalagfatvaqaapndntWyTgAkLgWsqyhdtgfinnngpthenqLgAgAfGgYqVnpyVgLeLgVdVlgrmpykgsvengaykaqgYqAtLkMgVpitddLdIyTrLgGmVwradtksnvygknhdtgvsPvFaGgVeYaItpeIaMrLeTqHtnnigdYPYDVPDYAtrpdnWmVsLgAsYrfgCCPGCCHHHHHH
Proper citation: RRID:Addgene_62648 Copy
Species: Synthetic
Genetic Insert: OmpA_H2368
Vector Backbone Description: Backbone Marker:DNA2.0; Backbone Size:4000; Vector Backbone:pJexpress414; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26199411
Comments: Amino acid sequence:
mkktaiaiavalagfatvaqaapndntFyLgLkMgIsqyhdtgfinnngpthenqLgAgAfGgYqVnpyVgFeMgYdWlgrmpykgsvengaykaqgYqAtLkMgVpitddVdVyLrLgImVwradtksnvygknhdtgvsPvFaGgVeYaItpeIaMrLeTqHtnnigdYPYDVPDYAtrpdnGmLsLgVsYrfgCCPGCCHHHHHH
Proper citation: RRID:Addgene_62651 Copy
Species: Synthetic
Genetic Insert: OmpA_H1678
Vector Backbone Description: Backbone Marker:DNA2.0; Backbone Size:4000; Vector Backbone:pJexpress414; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26199411
Comments: Amino acid sequence: mkktaiaiavalagfatvaqaapndntWyTgAkLgWsqyhdtgfinnngpthenqQgIgAfAgIqVnpyVgLeLgVdVlgrmpykgsvengaykaqgYqAtLkMgVpitddVdVyLrLgImVwradtksnvygknhdtgvsPvFaGgVeYaItpeIaTrLeYqWtnnigdYPYDVPDYAtrpdnGmLsLgVsYrfgCCPGCCHHHHHH
Proper citation: RRID:Addgene_62652 Copy
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Lim Lab; Vector Backbone:unknown-ColE1 ori; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26261213
Proper citation: RRID:Addgene_62823 Copy
Genetic Insert: gfp
Vector Backbone Description: Backbone Marker:Lim Lab; Vector Backbone:unknown-ColE1 ori; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26261213
Proper citation: RRID:Addgene_62821 Copy
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