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http://www.addgene.org/117789

Species: Danio rerio
Genetic Insert: Cre
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a gamma-crystalin promoter driving NLS-TagBFP. Online tools and cloning help available at . May work in additional species beyond those listed. Please note that some discrepancies were found between Addgene's quality control result and the depositor's provided sequence. The depositor noted that these discrepancies do NOT affect plasmid function.

Proper citation: RRID:Addgene_117789 Copy   

•   Source: Addgene

http://www.addgene.org/117700

Species: Streptococcus canis
Genetic Insert: Streptococcus canis Cas9
Vector Backbone Description: Backbone Marker:Oxford Genetics; Vector Backbone:OG3569; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30397647

Proper citation: RRID:Addgene_117700 Copy   

•   Source: Addgene

http://www.addgene.org/117790

Species: Danio rerio
Genetic Insert: Cre
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a gamma-crystalin promoter driving NLS-mRFP. Online tools and cloning help available at . May work in additional species beyond those listed. Please note that some discrepancies were found between Addgene's quality control result and the depositor's provided sequence. The depositor noted that these discrepancies do NOT affect plasmid function.

Proper citation: RRID:Addgene_117790 Copy   

•   Source: Addgene

http://www.addgene.org/11779

Genetic Insert: CAG promoters, GFP, rtTR-KRAB-2SM2, Tet-off
Vector Backbone Description: Backbone Size:12859; Vector Backbone:None; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16432520
Comments: Transgenes can be expressed from any RNA Pol II promoter as part of bicistronic unit comprising the KRAB-based repressor; tetO sequences are inserted into the vector LTR. Tet-on and Tet-off versions rely on repressors that bind in the absence or the presence of doxycycline, respectively. Addition of Pol III promoter-small hairpin RNA cassette allows for drug-controllable RNA interference (Tet-on shRNA). Cloning shRNA into pLVET, pLVCT, and pLVPT vectors: first clone shRNA into pLVTHM downstream of the tetO-H1 region. Then cut pLVTHM containing your shRNA with MscI-FspI and clone the insert containing the 3'LTR to target plasmid opened with MscI-FspI. FspI cuts into AmpR. This means for inverted clones AmpR will not be restored; after selection you will be left with clones with the shRNA cassette and functional AmpR. pLVTHM and packaging plasmid for this system are also available at Addgene http://www.addgene.org/rnaitools Please visit Trono lab website http://tronolab.epfl.ch to see frequently asked questions on cloning strategies and packaging.

Proper citation: RRID:Addgene_11779 Copy   

•   Source: Addgene

http://www.addgene.org/117772

Species: Danio rerio
Genetic Insert: 3x3 Stop sequence
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a gamma-crystalin promoter driving NLS-mRFP. Online tools and cloning help available at . May work in additional species beyond those listed. Please note that some discrepancies were found between Addgene's quality control result and the depositor's provided sequence. The depositor noted that these discrepancies do NOT affect plasmid function.

Proper citation: RRID:Addgene_117772 Copy   

•   Source: Addgene

http://www.addgene.org/117808

Species: Homo sapiens
Genetic Insert: TagRFP
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2958; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Online tools and cloning help available at . May work in additional species beyond those listed. Please visit https://www.biorxiv.org/content/10.1101/431627v2 for bioRxiv preprint.

Proper citation: RRID:Addgene_117808 Copy   

•   Source: Addgene

http://www.addgene.org/117807

Species: Homo sapiens
Genetic Insert: TagRFP
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2958; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Online tools and cloning help available at . May work in additional species beyond those listed. Please visit https://www.biorxiv.org/content/10.1101/431627v2 for bioRxiv preprint.

Proper citation: RRID:Addgene_117807 Copy   

•   Source: Addgene

http://www.addgene.org/117858

Species: Synthetic
Genetic Insert: mGFP
Vector Backbone Description: Backbone Size:33339; Vector Backbone:pAdeasy-1; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30482691

Proper citation: RRID:Addgene_117858 Copy   

•   Source: Addgene

http://www.addgene.org/117848

Species: Synthetic
Genetic Insert: promoter, RBS, tsPurple
Vector Backbone Description: Backbone Marker:iGEM Registry; Vector Backbone:BioBrick pSB1C3 vector; Vector Types:Synthetic Biology, Escherichia coli; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29760772

Proper citation: RRID:Addgene_117848 Copy   

•   Source: Addgene

http://www.addgene.org/117846

Species: Synthetic
Genetic Insert: promoter, RBS, aeBlue
Vector Backbone Description: Backbone Marker:iGEM Registry; Vector Backbone:BioBrick pSB1C3 vector; Vector Types:Synthetic Biology, Escherichia coli; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29760772

Proper citation: RRID:Addgene_117846 Copy   

•   Source: Addgene

http://www.addgene.org/11785

Species: Mus musculus
Genetic Insert: CnA
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBJ5-stop; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7523407

Proper citation: RRID:Addgene_11785 Copy   

•   Source: Addgene

http://www.addgene.org/11788

Species: Homo sapiens
Genetic Insert: NFAT2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10200; Vector Backbone:pREP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin

Proper citation: RRID:Addgene_11788 Copy   

•   Source: Addgene

http://www.addgene.org/11787

Species: Homo sapiens
Genetic Insert: CnA CnB
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5700; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9125515

Proper citation: RRID:Addgene_11787 Copy   

•   Source: Addgene

http://www.addgene.org/117817

Species: Homo sapiens
Genetic Insert: gal4/VP16
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2958; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Online tools and cloning help available at . gal4-VP16 should be used in conjunction with a UAS expression line. May work in additional species beyond those listed. Please visit https://www.biorxiv.org/content/10.1101/431627v2 for bioRxiv preprint.

Proper citation: RRID:Addgene_117817 Copy   

•   Source: Addgene

http://www.addgene.org/117811

Species: Homo sapiens
Genetic Insert: eGFP
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2958; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Online tools and cloning help available at . May work in additional species beyond those listed. Please visit https://www.biorxiv.org/content/10.1101/431627v2 for bioRxiv preprint.

Proper citation: RRID:Addgene_117811 Copy   

•   Source: Addgene

http://www.addgene.org/118059

Species: Renilla reniformis
Genetic Insert: Renilla
Vector Backbone Description: Backbone Marker:Self made; Derived from pBluescriptSK; Backbone Size:2343; Vector Backbone:pUPD3; Vector Types:Luciferase, Synthetic Biology, GBPart; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31836712
Comments: Insert can be released with BsaI

Proper citation: RRID:Addgene_118059 Copy   

•   Source: Addgene

http://www.addgene.org/118057

Species: Synthetic
Genetic Insert: Red Firefly Luciferase CDS
Vector Backbone Description: Backbone Marker:Diego Orzaez Lab; Backbone Size:3057; Vector Backbone:pUPD; Vector Types:Synthetic Biology, Domestication of DNA parts for GoldenBraid asembly; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31836712
Comments: Insert can be released with BsaI

Proper citation: RRID:Addgene_118057 Copy   

•   Source: Addgene

http://www.addgene.org/117927

Species: Homo sapiens
Genetic Insert: ADAR1-p150
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28208661

Proper citation: RRID:Addgene_117927 Copy   

•   Source: Addgene

http://www.addgene.org/117929

Species: Homo sapiens
Genetic Insert: ADAR2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28208661

Proper citation: RRID:Addgene_117929 Copy   

•   Source: Addgene

http://www.addgene.org/118064

Species: Synthetic
Genetic Insert: CMV:RedF:bGHF
Vector Backbone Description: Backbone Marker:Self made; Derived from pBluescriptSK; Backbone Size:2327; Vector Backbone:ColE1 vector Alpha2; Vector Types:Mammalian Expression, Luciferase, Synthetic Biology, Assembly of GB2.0 transcriptional units; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31836712
Comments: Insert can be released with BsmBI

Proper citation: RRID:Addgene_118064 Copy   

•   Source: Addgene