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Vector Backbone Description: Backbone Marker:Custom; Backbone Size:6000; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35145312
Comments: Digest vector with AgeI+NheI to clone any sequence between the ribozymes
Proper citation: RRID:Addgene_180184 Copy
Species: MS2 Phage
Genetic Insert: Maturation Protein
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:2200; Vector Backbone:pMX; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_180228 Copy
Species: MS2 Phage
Genetic Insert: Maturation Protein
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:2200; Vector Backbone:pMX; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_180229 Copy
Species: Homo sapiens
Genetic Insert: p50
Vector Backbone Description: Backbone Size:4722; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27559088
Proper citation: RRID:Addgene_180238 Copy
Species: Homo sapiens
Genetic Insert: HLA-A*24:02
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET24a+; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: https://www.ebi.ac.uk/ipd/imgt/hla/alleles/allele/?accession=HLA00050 provides allele sequence data. For detailed protocols of expression, purification and use to visualize antigen-specific T cells, please refer to Altman and Davis (2016) Curr. Protoc. Immunol. doi.org/10.1002/cpim.14. We recommend the E. coli BL21(DE3) strain for expression.
Proper citation: RRID:Addgene_180445 Copy
Species: Homo sapiens
Genetic Insert: HLA-B*08:01
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET24a+; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: https://www.ebi.ac.uk/ipd/imgt/hla/alleles/allele/?accession=HLA00146 provides allele sequence data. For detailed protocols of expression, purification and use to visualize antigen-specific T cells, please refer to Altman and Davis (2016) Curr. Protoc. Immunol. doi.org/10.1002/cpim.14. We recommend the E. coli BL21(DE3) strain for expression.
Proper citation: RRID:Addgene_180447 Copy
Species: Homo sapiens
Genetic Insert: HLA-A*01:01
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET24a+; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: https://www.ebi.ac.uk/ipd/imgt/hla/alleles/allele/?accession=HLA00001 provides allele sequence data. For detailed protocols of expression, purification and use to visualize antigen-specific T cells, please refer to Altman and Davis (2016) Curr. Protoc. Immunol. doi.org/10.1002/cpim.14. We recommend the E. coli BL21(DE3) strain for expression.
Proper citation: RRID:Addgene_180448 Copy
Species: Homo sapiens
Genetic Insert: SEPTIN2
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34854883
Proper citation: RRID:Addgene_180321 Copy
Species: Homo sapiens
Genetic Insert: SEPTIN9_v3
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34854883
Proper citation: RRID:Addgene_180322 Copy
Species: Homo sapiens
Genetic Insert: SEPTIN9_v1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34854883
Proper citation: RRID:Addgene_180328 Copy
Species: Homo sapiens
Genetic Insert: SEPTIN9_v1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34854883
Proper citation: RRID:Addgene_180329 Copy
Species: Homo sapiens
Genetic Insert: HLA-B*35:01
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET24a+; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: https://www.ebi.ac.uk/ipd/imgt/hla/alleles/allele/?accession=HLA00237 provides allele sequence data. For detailed protocols of expression, purification and use to visualize antigen-specific T cells, please refer to Altman and Davis (2016) Curr. Protoc. Immunol. doi.org/10.1002/cpim.14. We recommend the E. coli BL21(DE3) strain for expression.
Proper citation: RRID:Addgene_180450 Copy
Species: Homo sapiens
Genetic Insert: HLA-A*02:01 (CD8 mut)
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET24a+; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Mutation prevents binding of CD8 (Purbhoo et al. (2001) J. Biol. Chem. doi.org/10.1074/jbc.M102498200). See https://www.ebi.ac.uk/ipd/imgt/hla/alleles/allele/?accession=HLA00005 for allele sequence data. For detailed protocols of expression, purification and use to visualize antigen-specific T cells, please refer to Altman and Davis (2016) Curr. Protoc. Immunol. doi.org/10.1002/cpim.14. We recommend the E. coli BL21(DE3) strain for expression.
Proper citation: RRID:Addgene_180459 Copy
Species: Homo sapiens
Genetic Insert: SEPTIN9_v1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34854883
Proper citation: RRID:Addgene_180334 Copy
Species: Homo sapiens
Genetic Insert: SEPTIN9_v1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34854883
Proper citation: RRID:Addgene_180339 Copy
Genetic Insert: E2 Crimson
Vector Backbone Description: Backbone Marker:Jeffrey Barrick (Addgene plasmid # 110598); Vector Backbone:pBTK402; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35467987
Comments: Please note: Plasmid contains an 85bp discrepancy downstream of E2 Crimson at the BBa_B0015 Terminator compared to the depositor's provided sequence. This discrepancy is not known to affect plasmid function.
Proper citation: RRID:Addgene_180421 Copy
Species: Homo sapiens
Genetic Insert: SEPTIN2
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34854883
Proper citation: RRID:Addgene_180319 Copy
Species: Homo sapiens
Genetic Insert: SEPTIN9_v1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34854883
Proper citation: RRID:Addgene_180340 Copy
Species: Homo sapiens
Genetic Insert: HLA-B*40:01
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET24a+; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: https://www.ebi.ac.uk/ipd/imgt/hla/alleles/allele/?accession=HLA00291 provides allele sequence data. For detailed protocols of expression, purification and use to visualize antigen-specific T cells, please refer to Altman and Davis (2016) Curr. Protoc. Immunol. doi.org/10.1002/cpim.14. We recommend the E. coli BL21(DE3) strain for expression.
Proper citation: RRID:Addgene_180463 Copy
Vector Backbone Description: Vector Backbone:pGL198; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34837319
Proper citation: RRID:Addgene_180472 Copy
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