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Vector Backbone Description: Backbone Marker:Self made; Derived from pBluescriptSK; Backbone Size:2171; Vector Backbone:ColE1 vector Omega1; Vector Types:Mammalian Expression, Bacterial Expression, Insect Expression, Synthetic Biology, Assembly of GB2.0 transcriptional units; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31836712
Comments: This is a BmsBI GB2.0 Omega entry vector. Any assembled unit(s) can be released with BsaI for further cloning into Alfa level vectors.
Proper citation: RRID:Addgene_118046 Copy
Genetic Insert: SpCas9-NG (L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)
Vector Backbone Description: Vector Backbone:pX330; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30166441
Proper citation: RRID:Addgene_117919 Copy
Species: Rattus norvegicus
Genetic Insert: GABRD
Vector Backbone Description: Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16181787
Proper citation: RRID:Addgene_118037 Copy
Species: Homo sapiens
Genetic Insert: human IFT20
Vector Backbone Description: Vector Backbone:pCD-betaG-FLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_118033 Copy
Genetic Insert: sgRNA backbone (F+E)
Vector Backbone Description: Vector Backbone:pCESAx (Addgene 59986); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30580963
Comments: Users should follow the oligo cloning protocol for plasmid lentiCRISPR v2 (Addgene #52961) to 1) remove stuffer fragment and 2) insert annealed oligos to complete gRNA.
Proper citation: RRID:Addgene_117986 Copy
Species: Arabidopsis thaliana
Genetic Insert: CTP-mCitrine
Vector Backbone Description: Backbone Size:9204; Vector Backbone:pN_35S; Vector Types:Plant Expression, Plant/bacteria binary vector; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30884945
Comments: mCitrine contains an L7E compared to the published mCitrine sequence that does not impact plasmid function.
Proper citation: RRID:Addgene_117989 Copy
Vector Backbone Description: Backbone Marker:Self made; Derived from pBluescriptSK; Backbone Size:2804; Vector Backbone:pUPD; Vector Types:Synthetic Biology, Domestication of DNA parts for GoldenBraid asembly; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31836712
Comments: This is a GB2.0 domesticator vector. GBParts can be assembled in a BsmBI-ligation reaction. Any assembled Part can be released with BsaI for further cloning into Alpha level vectors.
Proper citation: RRID:Addgene_118043 Copy
Species: Homo sapiens
Genetic Insert: sgTP53-5
Vector Backbone Description: Backbone Marker:Feng Zhang Lab; Backbone Size:9482; Vector Backbone:pXPR003; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30224644
Comments: High efficiency of TP53 deletion
Proper citation: RRID:Addgene_118023 Copy
Species: Homo sapiens
Genetic Insert: sgTP53-4
Vector Backbone Description: Backbone Marker:Feng Zhang Lab; Backbone Size:9482; Vector Backbone:pXPR003; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30224644
Comments: High efficiency of TP53 deletion
Proper citation: RRID:Addgene_118022 Copy
Species: Synthetic
Genetic Insert: eGFP
Vector Backbone Description: Backbone Size:4935; Vector Backbone:pSIRV; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26780292
Proper citation: RRID:Addgene_118031 Copy
Genetic Insert: sfTq2ox-PBP5
Vector Backbone Description: Vector Backbone:pTHV037; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30648295
Proper citation: RRID:Addgene_117960 Copy
Species: Homo sapiens
Genetic Insert: TP53
Vector Backbone Description: Backbone Marker:Broad Institute; Backbone Size:8767; Vector Backbone:pLX313; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30224644
Proper citation: RRID:Addgene_118015 Copy
Species: S. pyrogenes
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:Broad Institute; Backbone Size:8767; Vector Backbone:pLX311; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30224644
Proper citation: RRID:Addgene_118018 Copy
Species: P. pyralis
Genetic Insert: Firefly luciferase
Vector Backbone Description: Backbone Marker:Broad Institute; Backbone Size:8767; Vector Backbone:pLX313; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30224644
Proper citation: RRID:Addgene_118017 Copy
Species: Mus musculus
Genetic Insert: NFAT2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2400; Vector Backbone:pENTR11; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_11793 Copy
Vector Backbone Description: Backbone Size:7650; Vector Backbone:pLL3.7; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12590264
Comments: Lentiviral vector that expresses shRNA under the mouse U6 promoter. A CMV-EGFP reporter cassette is included in the vector to monitor expression.
For protocols regarding the use of pLL3.7, visit: http://web.mit.edu/jacks-lab/protocols/rnairesources.htm
Note: A single base pair deletion at position 11 of the U6 promoter in this plasmid does not impair the efficacy of this reagent.
Proper citation: RRID:Addgene_11795 Copy
Species: Homo sapiens
Genetic Insert: TP53
Vector Backbone Description: Backbone Marker:Broad Institute; Backbone Size:8767; Vector Backbone:pLX313; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30224644
Proper citation: RRID:Addgene_118014 Copy
Genetic Insert: sfTq2ox
Vector Backbone Description: Vector Backbone:N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30648295
Proper citation: RRID:Addgene_117930 Copy
Species: Homo sapiens
Genetic Insert: OTUB1
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30282802
Proper citation: RRID:Addgene_118209 Copy
Species: Homo sapiens
Genetic Insert: KRAB-dCas9-P2A-BlastR
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene #52961); Vector Backbone:lentiCRISPRv2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Comments: We have also generated a version of this backbone with the hPGK promoter driving the expression of KRAB-dCas9. We advise users to test both backbones in their cell type of interest.
Note: Plasmid contains a N1396S mutation in dCas9. This mutation is not known to affect plasmid function.
Proper citation: RRID:Addgene_118154 Copy
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