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Species: Saccharomyces cerevisiae
Genetic Insert: TIF3
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:7474; Vector Backbone:pTYB2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20864040
Proper citation: RRID:Addgene_37227 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPL41A
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20864040
Comments: The sequence of RPL41A mRNA is as previously described (Yu and Warner, 2001). Two G bases were added at the 5´-ends of both mRNAs in order to promote efficient transcription and the sequence GGATC was added at the 3´-end as part of a BamHI site during cloning.
Proper citation: RRID:Addgene_37223 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: eIF1A
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:7474; Vector Backbone:pTYB2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17913637
Proper citation: RRID:Addgene_37218 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TIF4631
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4900; Vector Backbone:pGEX4t1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20864040
Proper citation: RRID:Addgene_37231 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TIF4631
Vector Backbone Description: Backbone Marker:EMD bioscience; Backbone Size:5420; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20864040
Proper citation: RRID:Addgene_37232 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PAB1
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:7474; Vector Backbone:pTYB2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21139564
Proper citation: RRID:Addgene_37234 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TIF1
Vector Backbone Description: Backbone Marker:EMD bioscience; Backbone Size:5441; Vector Backbone:pET21c; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20864040
Proper citation: RRID:Addgene_37230 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Met3p-mCherry-Tub1
Vector Backbone Description: Backbone Size:5504; Vector Backbone:pRS305; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19185494
Proper citation: RRID:Addgene_37553 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SNF7
Vector Backbone Description: Backbone Size:6098; Vector Backbone:pTO_LAP_N-DEST; Vector Types:Mammalian Expression, Bacterial Expression, Gateway cloning, Flp-FRT recombination, Tet-inducible expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28604678
Comments: Part of the plasmid was derived from pmCherry_N_dest from the Helenius Lab (ETH Zuerich)
Proper citation: RRID:Addgene_101851 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YGL225W
Vector Backbone Description: Backbone Marker:Simon Newstead (Addgene plasmid # 58352); Vector Backbone:pDDGFP-Leu2d; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29143814
Proper citation: RRID:Addgene_102334 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SEC16
Vector Backbone Description: Vector Backbone:p416TEF; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28476767
Proper citation: RRID:Addgene_102676 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: crADE2-3.S
Vector Backbone Description: Backbone Size:5058; Vector Backbone:pUD628; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29106617
Proper citation: RRID:Addgene_103018 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: crHCAN1-4.S
Vector Backbone Description: Backbone Marker:Świat M... Daran-Lapujade PAS FnCpf1, a novel and efficient genome editing tool for Saccharomyces cerevisiae. NAR; Backbone Size:4995; Vector Backbone:pUD628; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29106617
Proper citation: RRID:Addgene_103022 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: X-3 site gRNA expression cassettes
Vector Backbone Description: Backbone Size:5856; Vector Backbone:pYES2; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30199094
Comments: pYES2-gRNA-hyg-MCS is an E. coli-S. cerevisiae shuttle plasmid which harbors gRNA expression cassettes targeting S. cerevisiae chromosomal X-3 site gRNA (HXK1 gene).
However, it can function as an empty gRNA expression backbone plasmid because the existing gRNA expression cassette can be easily replaced with conventional restriction enzyme digestion-ligation method. Moreover, multiple gRNA expression cassettes can be loaded in the MCS.
Proper citation: RRID:Addgene_107734 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA-CAN1.Y, gRNA-ADE2.Y
Vector Backbone Description: Backbone Marker:George Church (Addgene plasmid # 43803); Backbone Size:5462; Vector Backbone:p426-SNR52p-gRNA.CAN1.Y-SUP4t; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25743786
Proper citation: RRID:Addgene_107930 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA-CAN1.Y
Vector Backbone Description: Backbone Marker:George Church (Addgene plasmid # 43803); Backbone Size:6130; Vector Backbone:p426-SNR52p-gRNA.CAN1.Y-SUP4t; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25743786
Proper citation: RRID:Addgene_107919 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA-CAN1.Y
Vector Backbone Description: Backbone Marker:George Church (Addgene plasmid # 43803); Backbone Size:6356; Vector Backbone:p426-SNR52p-gRNA.CAN1.Y-SUP4t; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25743786
Proper citation: RRID:Addgene_107918 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA-CAN1.Y
Vector Backbone Description: Backbone Marker:George Church (Addgene plasmid # 43803); Backbone Size:6928; Vector Backbone:p426-SNR52p-gRNA.CAN1.Y-SUP4t; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25743786
Proper citation: RRID:Addgene_107917 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA-CAN1.Y
Vector Backbone Description: Backbone Marker:George Church (Addgene plasmid # 43803); Backbone Size:6109; Vector Backbone:p426-SNR52p-gRNA.CAN1.Y-SUP4t; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25743786
Proper citation: RRID:Addgene_107916 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA-CAN1.Y
Vector Backbone Description: Backbone Marker:George Church (Addgene plasmid # 43803); Backbone Size:6945; Vector Backbone:p426-SNR52p-gRNA.CAN1.Y-SUP4t; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25743786
Proper citation: RRID:Addgene_107920 Copy
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