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Species: Homo sapiens
Genetic Insert: Serotonin Transporter (SERT)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10410; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://doi.org/10.1101/2021.04.19.440442 for bioRxiv preprint.
Proper citation: RRID:Addgene_107459 Copy
Species: Homo sapiens
Genetic Insert: Serotonin Transporter (SERT)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10410; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://doi.org/10.1101/2021.04.19.440442 for bioRxiv preprint.
Proper citation: RRID:Addgene_107472 Copy
Species: Homo sapiens
Genetic Insert: Serotonin Transporter (SERT)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10410; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://doi.org/10.1101/2021.04.19.440442 for bioRxiv preprint.
Proper citation: RRID:Addgene_107471 Copy
Species: Homo sapiens
Genetic Insert: Serotonin Transporter (SERT)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10410; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://doi.org/10.1101/2021.04.19.440442 for bioRxiv preprint.
Proper citation: RRID:Addgene_107469 Copy
Species: Homo sapiens
Genetic Insert: Serotonin Transporter (SERT)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10410; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://doi.org/10.1101/2021.04.19.440442 for bioRxiv preprint.
Proper citation: RRID:Addgene_107468 Copy
Species: Homo sapiens
Genetic Insert: MXRA7 (codon optimized)
Vector Backbone Description: Vector Backbone:pLenti CMV Puro DEST (w118-1); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30404791
Proper citation: RRID:Addgene_107500 Copy
Species: Homo sapiens
Genetic Insert: Serotonin Transporter (SERT)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10410; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://doi.org/10.1101/2021.04.19.440442 for bioRxiv preprint.
Proper citation: RRID:Addgene_107466 Copy
Species: Homo sapiens
Genetic Insert: Serotonin Transporter (SERT)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10410; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://doi.org/10.1101/2021.04.19.440442 for bioRxiv preprint.
Proper citation: RRID:Addgene_107465 Copy
Species: Homo sapiens
Genetic Insert: Serotonin Transporter (SERT)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10410; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://doi.org/10.1101/2021.04.19.440442 for bioRxiv preprint.
Proper citation: RRID:Addgene_107464 Copy
Species: Homo sapiens
Genetic Insert: Serotonin Transporter (SERT)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10410; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://doi.org/10.1101/2021.04.19.440442 for bioRxiv preprint.
Proper citation: RRID:Addgene_107463 Copy
Species: Homo sapiens
Genetic Insert: Serotonin Transporter (SERT)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10410; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://doi.org/10.1101/2021.04.19.440442 for bioRxiv preprint.
Proper citation: RRID:Addgene_107462 Copy
Species: Homo sapiens
Genetic Insert: CRIP2 (codon optimized)
Vector Backbone Description: Vector Backbone:pLenti CMV Puro DEST (w118-1); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30404791
Proper citation: RRID:Addgene_107509 Copy
Species: Homo sapiens
Genetic Insert: VOPP1
Vector Backbone Description: Vector Backbone:pLenti CMV Puro DEST (w118-1); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30404791
Proper citation: RRID:Addgene_107507 Copy
Species: Homo sapiens
Genetic Insert: RALB
Vector Backbone Description: Vector Backbone:pLenti CMV Puro DEST (w118-1); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30404791
Proper citation: RRID:Addgene_107506 Copy
Species: Homo sapiens
Genetic Insert: PTEN
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11707428
Proper citation: RRID:Addgene_10760 Copy
Species: Homo sapiens
Genetic Insert: PTEN
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pSG5L-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_10767 Copy
Species: Homo sapiens
Genetic Insert: AAVS1 Homology Arms with CAGGS-driven mTagRFPT-CAAX
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 to insert a CAGGS-mTagRFPT-CAAX at the AAVS1 safe harbor locus in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. After insertion of the reporter using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ .
Proper citation: RRID:Addgene_107580 Copy
Species: Homo sapiens
Genetic Insert: TARDBP mutant
Vector Backbone Description: Vector Backbone:pCS2-Dest; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31653829
Proper citation: RRID:Addgene_107799 Copy
Species: Homo sapiens
Genetic Insert: TARDBP mutant
Vector Backbone Description: Vector Backbone:pCS2-Dest; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_107798 Copy
Species: Homo sapiens
Genetic Insert: gRNA targeting human DNM1
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 48139); Backbone Size:9200; Vector Backbone:PX459, pSpCAS9n(BB)-2A-puro; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29668686
Comments: Created using DNA assembly in yeast
Proper citation: RRID:Addgene_107796 Copy
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