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Species: Homo sapiens
Genetic Insert: TRAV
Vector Backbone Description: Backbone Size:9531; Vector Backbone:pLX301 (Addgene #25895); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33483338
Proper citation: RRID:Addgene_165005 Copy
Species: Homo sapiens
Genetic Insert: OCT4
Vector Backbone Description: Vector Backbone:pMaster; Vector Types:Mammalian Expression, Episomal; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34088009
Proper citation: RRID:Addgene_165080 Copy
Species: Homo sapiens
Genetic Insert: OCT4
Vector Backbone Description: Vector Backbone:pMaster; Vector Types:Mammalian Expression, Episomal; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34088009
Comments: Please note that Addgene's NGS found that the bGH poly(A) signal between one of the oris and Lin28 is absent as compared to the annotated sequence provided by the depositor.
Please note that this plasmid contains repetitive elements which are prone to recombination. Screen 3-5 small colonies to ensure the full plasmid is selected. For example, the restriction enzyme NheI can be used to check the plasmid size as shown in Addgene's Diagnostic Digest.
Proper citation: RRID:Addgene_165081 Copy
Species: Homo sapiens
Genetic Insert: TRB
Vector Backbone Description: Backbone Size:9531; Vector Backbone:pLX301 (Addgene #25895); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33483338
Proper citation: RRID:Addgene_165008 Copy
Species: Homo sapiens
Genetic Insert: Rheb-4Rs
Vector Backbone Description: Backbone Marker:Available at Addgene (#19319); Backbone Size:7400; Vector Backbone:pLJM1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33157014
Proper citation: RRID:Addgene_165022 Copy
Species: Homo sapiens
Genetic Insert: HLA-B*51:01
Vector Backbone Description: Backbone Size:9335; Vector Backbone:pLX303 (Addgene #25897); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33483338
Proper citation: RRID:Addgene_165011 Copy
Species: Homo sapiens
Genetic Insert: HLA-DRA
Vector Backbone Description: Backbone Size:9335; Vector Backbone:pLX303 (Addgene #25897); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33483338
Proper citation: RRID:Addgene_165012 Copy
Species: Homo sapiens
Genetic Insert: HLA-A*24:02
Vector Backbone Description: Backbone Size:9335; Vector Backbone:pLX303 (Addgene #25897); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33483338
Proper citation: RRID:Addgene_165010 Copy
Species: Homo sapiens
Genetic Insert: FAST-1 H83R
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5500; Vector Backbone:pCI-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9702198
Proper citation: RRID:Addgene_16522 Copy
Species: Homo sapiens
Genetic Insert: FAST-1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5500; Vector Backbone:pCI-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9702198
Comments: To construct an hFAST-1 expression vector, normal human colon cDNA was used as the template to PCR-amplify the hFAST-1 ORF with primers NT2-exp5′ (5′-TATGCGGCCGCCACCATGGGGCCCTGCAGCG-3′) and NT2-exp3′ (5′-TATGCGGCCGCGAGCTGCTGTGTCGCAGAC-3′). The PCR product was cloned into the NotI site of pCI-neo.
Proper citation: RRID:Addgene_16521 Copy
Species: Homo sapiens
Genetic Insert: beta catenin
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5500; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9065402
Comments: HCT116 has a 3-bp deletion that removes one amino acid (Ser45).
Proper citation: RRID:Addgene_16520 Copy
Species: Homo sapiens
Genetic Insert: mCherry-ACTB-P2A-EGFP
Vector Backbone Description: Backbone Marker:Scott Lowe; Backbone Size:5795; Vector Backbone:pLPC; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33662274
Proper citation: RRID:Addgene_165158 Copy
Species: Homo sapiens
Genetic Insert: E6AP Hect
Vector Backbone Description: Backbone Marker:GE Healthcare; Backbone Size:4900; Vector Backbone:pGEX4T-3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22350887
Comments: Best yields for protein purification were found in BL21 (DE) cells using an overnight induction at 16 degrees C.
Proper citation: RRID:Addgene_165102 Copy
Species: Homo sapiens
Genetic Insert: E6AP isoform 3
Vector Backbone Description: Backbone Marker:GE Healthcare; Backbone Size:4900; Vector Backbone:pGEX4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23439649
Comments: Best yields for protein purification were found in BL21 (DE) cells using an overnight induction at 16 degrees C.
Proper citation: RRID:Addgene_165100 Copy
Species: Homo sapiens
Genetic Insert: Nedd4.2d
Vector Backbone Description: Backbone Marker:GE Healthcare; Backbone Size:4900; Vector Backbone:pGEX6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28972136
Comments: Best yields for protein purification were found in BL21 (DE) cells using an overnight induction at 16 degrees C.
Proper citation: RRID:Addgene_165103 Copy
Species: Homo sapiens
Genetic Insert: Nedd4.2d F823A
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:7013; Vector Backbone:pDEST15; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30287686
Comments: Best yields for protein purification in BL21 AI cells using an overnight induction at 16 degrees C.
5' Cloning Site: BamHI (not destroyed), 3' Cloning Site: EcoRI (not destroyed).
Proper citation: RRID:Addgene_165104 Copy
Species: Homo sapiens
Genetic Insert: p53 binding site
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:4900; Vector Backbone:pBV-Luc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9659898
Comments: This reporter contains 3 copies of the wt p53-binding site from the 14-3-3 sigma promoter. The following oligonucleotide pairs were used: 5'-CCTGTAGCAT TAGCCCAGAC ATGTCCCTAC TCCGTAC-3' and 5'-GGAGTAGGGA CATGTCTGGG CTAATGCTAC AGGGTAC-3' for BDS-2.
Proper citation: RRID:Addgene_16515 Copy
Species: Homo sapiens
Genetic Insert: TCF4
Vector Backbone Description: Backbone Size:9200; Vector Backbone:pHR; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9065401
Comments: The TCF4 in this construct has a Q27R mutation. It is not known to affect the function.
According to the depositing scientist, other mutations may be present but they have no functional consequences on standard assays for Tcf4 activity.
Proper citation: RRID:Addgene_16514 Copy
Species: Homo sapiens
Genetic Insert: TCF4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:0; Vector Backbone:pcDNA?; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9065401
Comments: The TCF4 in this construct has a Q27R mutation. It is not known to affect the function.
Proper citation: RRID:Addgene_16512 Copy
Species: Homo sapiens
Genetic Insert: beta catenin
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5500; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9065402
Comments: SW48 has a C-to-A missense mutation that changes Ser33 to Tyr.
Note that the NheI site is not intact and cannot be used for sub-cloning.
Proper citation: RRID:Addgene_16519 Copy
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