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Species: Saccharomyces cerevisiae
Genetic Insert: BirA-heh2-Ssh1
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25378630
Proper citation: RRID:Addgene_62363 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: BirA-mVenus-Ubc6 tail anchor
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25378630
Proper citation: RRID:Addgene_62364 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: BirA-mVenus-Ssh1
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25378630
Proper citation: RRID:Addgene_62362 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: URA3 3' fragment
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:2961; Vector Backbone:pBluescript II SK (-); Vector Types:Routine cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24604451
Comments: pJH131 was created by cloning an NheI/SacII GAL10/GAL1 promoter fragment into the same sites of pJH124. The cloned insert was confirmed by DNA sequencing.
Proper citation: RRID:Addgene_48260 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: dCas9_VP64 (codon-optimized for expression in S. cerevisiae)
Vector Backbone Description: Vector Backbone:pTPGI (pRS304); Vector Types:Yeast Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23977949
Proper citation: RRID:Addgene_49013 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RFA3
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5700; Vector Backbone:pET11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8910442
Proper citation: RRID:Addgene_49339 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Inducible peptide of the dPSTR
Vector Backbone Description: Backbone Size:5053; Vector Backbone:pRS305; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27098003
Comments: This intermediate cloning vector is used to change the measured promoter (here pSTL1), by cloning it between SacI (or AatII) and XbaI (or SpeI). The entire inducible peptide has to be inserted into a pSIV by cloning AatII / SphI (into pDA183 or pDA200 for instance).
For more information, see Aymoz et al. Nat. Comm. 2016.
Proper citation: RRID:Addgene_74251 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Inducible peptide of the dPSTR
Vector Backbone Description: Backbone Size:5053; Vector Backbone:pRS305; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27098003
Comments: This intermediate cloning vector is used to change the measured promoter (here pGPD1), by cloning it between SacI (or AatII) and XbaI (or SpeI). The entire inducible peptide has to be inserted into a pSIV by cloning AatII / SphI (into pDA183 or pDA200 for instance).
For more information, see Aymoz et al. Nat. Comm. 2016.
Proper citation: RRID:Addgene_74250 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pGPD1-dPSTRy(4,3)
Vector Backbone Description: Vector Backbone:pSIVL; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27098003
Comments: This vector contains the complete pGPD1-dPSTRy(4,3). The vector can be cut either PacI or BstBI prior to transformation in yeast. Selection in Leu depleted medium.
For more information, see Aymoz et al. Nat. Comm. 2016.
Proper citation: RRID:Addgene_74248 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pSTL1-dPSTRy(4,3)
Vector Backbone Description: Vector Backbone:pSIVL; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27098003
Comments: This vector contains the complete pSTL1-dPSTRy(4,3). The vector can be cut either PacI or BstBI prior to transformation in yeast. Selection in Leu depleted medium.
For more information, see Aymoz et al. Nat. Comm. 2016.
Proper citation: RRID:Addgene_74275 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pH-tdGFP
Vector Backbone Description: Backbone Size:4150; Vector Backbone:pFA6a; Vector Types:Yeast Expression, yeast tagging; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27324986
Proper citation: RRID:Addgene_74322 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ret2LD2a K153Q
Vector Backbone Description: Vector Backbone:p415; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27298352
Proper citation: RRID:Addgene_75261 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ret2LD2a T158K
Vector Backbone Description: Vector Backbone:p415; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27298352
Proper citation: RRID:Addgene_75262 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MAK10TR
Vector Backbone Description: Backbone Marker:Solis-Escalante et.al FEMS Yeast Res 13 (2013) 126–139; Backbone Size:4807; Vector Backbone:pUG amdSYM; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_75433 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sup35R1+5
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pRS306; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17893150
Proper citation: RRID:Addgene_17718 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pRS306Sup35R1
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pRS306; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17893150
Proper citation: RRID:Addgene_17715 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sup35R1+2
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pRS306; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17893150
Proper citation: RRID:Addgene_17716 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sup35R1+4
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pRS306; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17893150
Proper citation: RRID:Addgene_17717 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sup35R1+7
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pRS306; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17893150
Proper citation: RRID:Addgene_17720 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sup35R1+8
Vector Backbone Description: Backbone Size:2300; Vector Backbone:pJC25; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17893150
Proper citation: RRID:Addgene_17724 Copy
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