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Species: Homo sapiens
Genetic Insert: H2B-MiniR-mEm
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:5863; Vector Backbone:mEmerald-H2B-6; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32786411
Proper citation: RRID:Addgene_181990 Copy
Species: Other
Genetic Insert: FRT
Vector Backbone Description: Vector Backbone:pENTR; Vector Types:pENTR plasmid; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35143616
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.11.18.469106v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_182163 Copy
Species: Homo sapiens
Genetic Insert: mEm-CoilE-Actin
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:6643; Vector Backbone:mEmerald-Actin-C-18; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30518560
Proper citation: RRID:Addgene_181993 Copy
Species: Homo sapiens
Genetic Insert: H2B-CoilE-mEm
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:5863; Vector Backbone:mEmerald-H2B-6; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30518560
Proper citation: RRID:Addgene_181992 Copy
Species: Homo sapiens
Genetic Insert: TOMM20-CoilE-mCherry
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:5923; Vector Backbone:mCherry-TOMM20-N-10; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30518560
Proper citation: RRID:Addgene_181995 Copy
Species: Synthetic
Genetic Insert: attP-GA-EGFP-EF1α-PuroR-T2A-TagBFP
Vector Backbone Description: Vector Backbone:pEF1α; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34887556
Proper citation: RRID:Addgene_182140 Copy
Species: Synthetic
Genetic Insert: MiniE-DBCO
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5368; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32786411
Proper citation: RRID:Addgene_181985 Copy
Species: Arabidopsis thaliana
Genetic Insert: MUM2 signal peptide, fused to the Citrine YFP in pAD
Vector Backbone Description: Backbone Marker:DeBono 2011; Backbone Size:5000; Vector Backbone:pAD; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34877448
Proper citation: RRID:Addgene_181963 Copy
Species: Synthetic
Genetic Insert: attP-EGFP-EF1α-PuroR-T2A-TagBFP
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34887556
Proper citation: RRID:Addgene_182139 Copy
Species: Synthetic
Genetic Insert: mVenusNB-MrkHR113A
Vector Backbone Description: Backbone Marker:J. H. Davis, A. J. Rubin, R. T. Sauer, Design, construction and characterization of a set of insulated bacterial promoters.; Backbone Size:3200; Vector Backbone:pEB2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35131853
Comments: We obtained the codon optimized MrkH sequence from Maria Schumacher, as described in her publication PMID: 27551088
Proper citation: RRID:Addgene_182292 Copy
Species: Brevibacillus sp. SYP-B805
Genetic Insert: BrCas12b
Vector Backbone Description: Backbone Marker:Scott Gradia Lab; Backbone Size:6462; Vector Backbone:pET; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35290826
Proper citation: RRID:Addgene_182276 Copy
Species: Synthetic
Genetic Insert: GFP 1-9 OPT
Vector Backbone Description: Backbone Marker:pEGFP (Clontech); Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24092409
Proper citation: RRID:Addgene_182244 Copy
Genetic Insert: mCherry-Fis1
Vector Backbone Description: Vector Backbone:pmCherry; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32778832
Proper citation: RRID:Addgene_182580 Copy
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET24; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Primers for LIC cloning: Upstream: add TATTTTCAATCCTACGTA to the 5’ end of gene of interest. Downstream: add CCCTCAATATTATACGGG to the 3’ end of GOI. Detailed cloning method available in the paper Bruni R, Kloss B. High-throughput cloning and expression of integral membrane proteins in Escherichia coli. Curr Protoc Protein Sci. 2013 Nov 5;74:29.6.1-29.6.34.
Proper citation: RRID:Addgene_182506 Copy
Species: Mus musculus
Genetic Insert: glyceraldehyde-3-phosphate dehydrogenase
Vector Backbone Description: Backbone Size:3937; Vector Backbone:pDendra2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34996455
Proper citation: RRID:Addgene_182368 Copy
Species: Rattus norvegicus
Genetic Insert: rat LC3
Vector Backbone Description: Vector Backbone:mEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34494547
Proper citation: RRID:Addgene_182865 Copy
Species: Other
Genetic Insert: GPI-mApple
Vector Backbone Description: Vector Backbone:mEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34494547
Proper citation: RRID:Addgene_182868 Copy
Species: Homo sapiens
Genetic Insert: human CD9
Vector Backbone Description: Vector Backbone:mEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34494547
Proper citation: RRID:Addgene_182864 Copy
Species: Homo sapiens
Genetic Insert: Golgi-mEGFP
Vector Backbone Description: Vector Backbone:Golgi-mTurquoise; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33239451
Proper citation: RRID:Addgene_182877 Copy
Species: Homo sapiens
Genetic Insert: hEGFR-mEGFP
Vector Backbone Description: Vector Backbone:EGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32455962
Proper citation: RRID:Addgene_182878 Copy
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