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Species: Homo sapiens
Genetic Insert: DNM2-mRuby2
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:5616; Vector Backbone:pRS424; Vector Types:Bacterial Expression, Yeast Expression, yeast-E.coli shuttle vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29668686
Comments: Created using DNA assembly in yeast
Proper citation: RRID:Addgene_107793 Copy
Species: Homo sapiens
Genetic Insert: MAPRE1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3941; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29379139
Comments: LOV2 domain starts with LAAA reported to stabilize Jα helix docking (Strickland et al. PMID: 22388287) and mutations I427V and H519L resulting in faster dark state recovery.
This LOV2 domain has the following mutations: I427V, H519L. The I427V mutation increases the dark state reversion rate of the LOV2 domain. Zdk1 binds efficiently to this LOV2 domain.
Proper citation: RRID:Addgene_107690 Copy
Species: Homo sapiens
Genetic Insert: MAPRE3 gRNA #1 (targets Exon 1)
Vector Backbone Description: Backbone Marker:Feng Zhang Lab; Backbone Size:9288; Vector Backbone:pSpCas9(BB)-2A-GFP (Addgene #48138); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29379139
Comments: For a detailed protocol on knocking out EB1 in human cell lines by CRISPR/Cas9, see our protocol on "Protocol Exchange" (doi and link can be found below)
For rapidly cloning gRNA sequences into pSpCAS9(BB)-2a-GFP in a single RE digestion/ ligation step, see support protocol 3
Generation of cell lines with light-controlled microtubule dynamics
Torsten Wittmann & Jeffrey van Haren
Protocol Exchange (2018) doi:10.1038/protex.2017.155
https://www.nature.com/protocolexchange/protocols/6427
Proper citation: RRID:Addgene_107729 Copy
Species: Homo sapiens
Genetic Insert: MAPRE1 gRNA #1 (targets Exon 1)
Vector Backbone Description: Backbone Marker:Feng Zhang Lab; Backbone Size:9288; Vector Backbone:pSpCas9(BB)-2A-GFP (Addgene #48138); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29379139
Comments: For a detailed protocol on knocking out EB1 in human cell lines by CRISPR/Cas9, see our protocol on "Protocol Exchange" (doi and link can be found below)
For rapidly cloning gRNA sequences into pSpCAS9(BB)-2a-GFP in a single RE digestion/ ligation step, see support protocol 3
Generation of cell lines with light-controlled microtubule dynamics
Torsten Wittmann & Jeffrey van Haren
Protocol Exchange (2018) doi:10.1038/protex.2017.155
https://www.nature.com/protocolexchange/protocols/6427
Proper citation: RRID:Addgene_107726 Copy
Species: Homo sapiens
Genetic Insert: PTEN
Vector Backbone Description: Backbone Size:4948; Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_10768 Copy
Species: Homo sapiens
Genetic Insert: PTEN
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pSG5L-Flag-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_10775 Copy
Species: Homo sapiens
Genetic Insert: TARDBP mutant
Vector Backbone Description: Vector Backbone:pCS2-Dest; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31653829
Proper citation: RRID:Addgene_107804 Copy
Species: Homo sapiens
Genetic Insert: TARDBP mutant
Vector Backbone Description: Vector Backbone:pCS2-Dest; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_107803 Copy
Species: Homo sapiens
Genetic Insert: Subunit C of SDH fused to pHluorin
Vector Backbone Description: Backbone Marker:NEB Biosciences; Backbone Size:5052; Vector Backbone:pSems26b; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28383048
Proper citation: RRID:Addgene_107767 Copy
Species: Homo sapiens
Genetic Insert: MAPRE3 gRNA #3 (targets Exon 1)
Vector Backbone Description: Backbone Marker:Feng Zhang Lab; Backbone Size:9288; Vector Backbone:pSpCas9(BB)-2A-GFP (Addgene #48138); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29379139
Comments: For a detailed protocol on knocking out EB1 in human cell lines by CRISPR/Cas9, see our protocol on "Protocol Exchange" (doi and link can be found below)
For rapidly cloning gRNA sequences into pSpCAS9(BB)-2a-GFP in a single RE digestion/ ligation step, see support protocol 3
Generation of cell lines with light-controlled microtubule dynamics
Torsten Wittmann & Jeffrey van Haren
Protocol Exchange (2018) doi:10.1038/protex.2017.155
https://www.nature.com/protocolexchange/protocols/6427
Proper citation: RRID:Addgene_107731 Copy
Species: Homo sapiens
Genetic Insert: MAPRE3 gRNA #2 (targets Exon 1)
Vector Backbone Description: Backbone Marker:Feng Zhang Lab; Backbone Size:9288; Vector Backbone:pSpCas9(BB)-2A-GFP (Addgene #48138); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29379139
Comments: For a detailed protocol on knocking out EB1 in human cell lines by CRISPR/Cas9, see our protocol on "Protocol Exchange" (doi and link can be found below)
For rapidly cloning gRNA sequences into pSpCAS9(BB)-2a-GFP in a single RE digestion/ ligation step, see support protocol 3
Generation of cell lines with light-controlled microtubule dynamics
Torsten Wittmann & Jeffrey van Haren
Protocol Exchange (2018) doi:10.1038/protex.2017.155
https://www.nature.com/protocolexchange/protocols/6427
Proper citation: RRID:Addgene_107730 Copy
Species: Homo sapiens
Genetic Insert: MAPRE1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3988; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29379139
Proper citation: RRID:Addgene_107696 Copy
Species: Homo sapiens
Genetic Insert: PTEN
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pSG5L-Flag-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_10786 Copy
Species: Homo sapiens
Genetic Insert: TARDBP mutant
Vector Backbone Description: Vector Backbone:pCS2-Dest; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31653829
Proper citation: RRID:Addgene_107831 Copy
Species: Homo sapiens
Genetic Insert: TARDBP mutant
Vector Backbone Description: Vector Backbone:pCS2-Dest; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31653829
Proper citation: RRID:Addgene_107830 Copy
Species: Homo sapiens
Genetic Insert: TARDBP mutant
Vector Backbone Description: Vector Backbone:pCS2-Dest; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_107835 Copy
Species: Homo sapiens
Genetic Insert: PTEN
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pSG5L-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_10788 Copy
Species: Homo sapiens
Genetic Insert: TARDBP mutant
Vector Backbone Description: Vector Backbone:pCS2-Dest; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_107834 Copy
Species: Homo sapiens
Genetic Insert: TARDBP mutant
Vector Backbone Description: Vector Backbone:pCS2-Dest; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_107833 Copy
Species: Homo sapiens
Genetic Insert: TARDBP mutant
Vector Backbone Description: Vector Backbone:pBI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_107841 Copy
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