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Species: Synthetic
Genetic Insert: IV-GGT
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_43774 Copy
Species: Synthetic
Genetic Insert: IV-GTA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_43775 Copy
Species: Homo sapiens
Genetic Insert: WNT2B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24841207
Proper citation: RRID:Addgene_43808 Copy
Species: Synthetic
Genetic Insert: IV-GCA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_43767 Copy
Genetic Insert: TALE-activator p65 backbone
Vector Backbone Description: Backbone Marker:Joung lab; Vector Backbone:unknown; Vector Types:TALE Transcriptional Activator Expression Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23396285
Comments: Location of plasmid features:
EF1a = 922-2106
start codon = 2119-2121
NLS = 2122-2165
N-term = 2166-2577
0.5 domain = 2592-2658
C-term = 2665-2949
p65 = 2959-3768
pA = 3775-3894
Proper citation: RRID:Addgene_43889 Copy
Species: Homo sapiens
Genetic Insert: Human Optimized S. pyogenes Cas9
Vector Backbone Description: Backbone Size:6670; Vector Backbone:p415; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23460208
Proper citation: RRID:Addgene_43804 Copy
Species: Mus musculus
Genetic Insert: Hes1 Promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5597; Vector Backbone:pGL2-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7906273
Proper citation: RRID:Addgene_43806 Copy
Genetic Insert: SID4X Domain
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:2710; Vector Backbone:pUC57; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22828628
Comments: This enhanced SID domain (SID4X domain) is to be used with TALE effectors to create TALE repressors, as described in Cong et al. Nature Communications. This new domain replaces the previous SID domain reported in the original article.
Proper citation: RRID:Addgene_43882 Copy
Genetic Insert: TALE-activator VP64 Backbone
Vector Backbone Description: Backbone Marker:Joung lab; Vector Backbone:unknown; Vector Types:TALE Transcriptional Activator Expression Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23396285
Comments: Location of plasmid features:
EF1a = 922-2106
start codon - 2119-2121
NLS = 2122-2165
N-term =2166-2577
0.5 domain = 2592-2658
C-term = 2665-2949
VP64 = 2956-3122
pA = 3125-3244
Proper citation: RRID:Addgene_43885 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: DIF1
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4898; Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18851834
Comments: The following mutations were found during the Addgene QC sequencing: Q31 deletion, S60G and Q68H.
Proper citation: RRID:Addgene_43909 Copy
Vector Backbone Description: Backbone Marker:Novagen, EMBL; Backbone Size:6841; Vector Backbone:pET, pETM44; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23410102
Comments: Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected.
Proper citation: RRID:Addgene_43986 Copy
Vector Backbone Description: Backbone Marker:Novagen, EMBL; Backbone Size:7237; Vector Backbone:pET, pETM14; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23410102
Comments: Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected.
Proper citation: RRID:Addgene_43987 Copy
Species: Homo sapiens
Genetic Insert: USP44
Vector Backbone Description: Backbone Size:9600; Vector Backbone:pDEST-MYC; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:17443180
Proper citation: RRID:Addgene_43901 Copy
Vector Backbone Description: Backbone Marker:Novagen, EMBL; Backbone Size:6660; Vector Backbone:pET, pET28M-Sumo1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23410102
Comments: Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected.
Proper citation: RRID:Addgene_43989 Copy
Species: Homo sapiens
Genetic Insert: USP44
Vector Backbone Description: Backbone Size:4700; Vector Backbone:p142; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17443180
Proper citation: RRID:Addgene_43903 Copy
Species: Homo sapiens
Genetic Insert: USP44
Vector Backbone Description: Backbone Size:9692; Vector Backbone:p332; Vector Types:Insect Expression, baculoviral expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17443180
Proper citation: RRID:Addgene_43905 Copy
Species: Homo sapiens
Genetic Insert: TNF receptor-associated factor 3
Vector Backbone Description: Backbone Marker:Shao-Cong Sun; Vector Backbone:pcDNA-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15084608
Comments: pCDNA-HA backbone: Inserted influenza hemagglutinin (HA) epitope tag (YPYDVPDYA) with the translation initiation codon (ATG) into pcDNA3.1 vector (Invitrogen); described in Harhaj, et al. (1999) JBC, 274, 22911–22914.
Please note that the hTRAF3 insert in this plasmid contains V274D and S393G mutations when compared to GenBank entry NP_663777.1
Proper citation: RRID:Addgene_44034 Copy
Species: Homo sapiens
Genetic Insert: TNF receptor-associated factor 3
Vector Backbone Description: Backbone Marker:Shao-Cong Sun; Vector Backbone:pcDNA-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15084608
Comments: pCDNA-HA backbone: Inserted influenza hemagglutinin (HA) epitope tag (YPYDVPDYA) with the translation initiation codon (ATG) into pcDNA3.1 vector (Invitrogen); described in Harhaj, et al. (1999) JBC, 274, 22911–22914.
Please note that the hTRAF3 insert in this plasmid contains V274D and S393G mutations when compared to GenBank entry NP_663777.1
Proper citation: RRID:Addgene_44033 Copy
Genetic Insert: AGA1
Vector Backbone Description: Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9181578
Proper citation: RRID:Addgene_44036 Copy
Vector Backbone Description: Backbone Marker:Novagen,; Backbone Size:6091; Vector Backbone:pET, pCoofy2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23410102
Comments: Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected.
Proper citation: RRID:Addgene_43982 Copy
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