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  • Organism:homo sapiens (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pBJ1 neo-hu FceRI gamma
 
Resource Report
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1+ mentions
RRID:Addgene_16540 FCER1G Homo sapiens Ampicillin PMID:2138619 This construct contains only the coding sequence. It was first used in Lin S, Cicala C, Scharenberg AM, Kinet J-P (1996) Cell 85, 985-995. It can be sequenced with primers within the insert. Backbone Marker:Mark M Davis; Backbone Size:6600; Vector Backbone:pBJ1 neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:28 1
pGEX4T-1-Optimized UBE1L
 
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RRID:Addgene_165099 UbeE1L Homo sapiens Ampicillin Best yields for protein purification were found in BL21 (DE) cells using an overnight induction at 16 degrees C. Backbone Marker:GE Healthcare; Backbone Size:4900; Vector Backbone:pGEX4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin codon optimized for E. coli expression 2026-08-15 01:09:27 0
CMV-ShadowY-Rhotekin(8-89)-P2A-Clover(T153M/F223R)-RhoA
 
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RRID:Addgene_165435 ShadowY-RBD-P2A-Clover(T153M/F223R)-RhoA Homo sapiens Ampicillin PMID:33531495 Vector Backbone:Customized Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:29 0
CMV-ShadowY-CBD-P2A-Clover(T153M/F223R)-Cdc42
 
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RRID:Addgene_165434 ShadowY-CBD-P2A-Clover(T153M/F223R)-Cdc42 Homo sapiens Ampicillin PMID:33531495 Vector Backbone:Customized Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:29 0
pCMV3Tag8li-hHP1BP3-HA
 
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RRID:Addgene_165468 Heterochromatin protein 1 binding protein 3 Homo sapiens Ampicillin PMID:24778252 Backbone contains new multiple cloning site and c-terminal HA epitope instead of FLAGs. Construction: XhoI-ACC-[HP1BP3 CDS 1650 bp, no stop]-Noti-a-Clai-HA epitope tag-ctt-STOP. T3 forward primer is too close for sequencing of initiation sequence. Backbone Marker:Stratagene /Agilent; Backbone Size:5200; Vector Backbone:pCMV-3Tag-8 modified MCS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:29 0
TANGO1S-mScarlet-i
 
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1+ mentions
RRID:Addgene_165461 TANGO1S Homo sapiens Ampicillin PMID:34350936 Backbone Marker:Clontech; Vector Backbone:pIRES_neo3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:29 1
AAVS1-Puro-CAG-fl-STOP-fl-ZEB2-GFP-Flag-WPRE-poly(A)
 
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RRID:Addgene_165456 ZEB2 Homo sapiens Ampicillin PMID:33765444 Backbone Size:11520; Vector Backbone:AAVS1-Puro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:29 0
pCMV3Tag8li-hMBOAT7-3xFLAG
 
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RRID:Addgene_165474 Membrane bound O-acyltransferase domain containing 7 Homo sapiens Ampicillin PMID:21903422 Backbone contains new MCS (multiple cloning site). CDS without stop codon was inserted with XhoI-NotI and it expresses in frame with 3xFLAG epitope tag. Construct insertion = Xhoi-GCC-[MBOAT7 CDS 1416 bp, no stop]-Noti-A-Clai-GTCGAG-3xFLAG-STOP Backbone Marker:Stratagene /Agilent; Backbone Size:5200; Vector Backbone:pCMV-3Tag-8 modified MCS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:29 0
pCMV3Tag8li-hADARB2-3xFLAG
 
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RRID:Addgene_165470 Adenosine deaminase RNA specific B2 (inactive) Homo sapiens Ampicillin PMID:24778252 Backbone contains new MCS (multiple cloning site). MCS is changed to: ctcgagctgaagcttcatggatccaaagcggccgcaatcgat (XhoI-ctg-HindIII-cat-BamHI-aaa-NotI-a-ClaI). CDS without stop codon was inserted into MCS at unkown restriction enzyme sites, and it expresses in frame with 3xFLAG epitope tag. Xhoi was probably the 5' cloning site. Backbone Marker:Stratagene /Agilent; Backbone Size:5200; Vector Backbone:pCMV-3Tag-8 modified MCS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:29 0
pGST-PPARgamma
 
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1+ mentions
RRID:Addgene_16549 PPAR gamma Homo sapiens Ampicillin PMID:10555149 Note that there are some discrepancies between the insert in this plasmid and the canonical GenBank PPARgamma. The Vogelstein lab maintains that this plasmid worked as specified when they made and used it. Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX-2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin N-terminal DNA-binding domain: aa 1-248 2026-08-15 01:09:29 2
pSico_U6-PGC1a1 sgRNA
 
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RRID:Addgene_165425 gRNA against PGC-1a variant 1 Homo sapiens Ampicillin PMID:33626346 Backbone Size:9580; Vector Backbone:pSico; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:09:28 0
pBI-p73 wt/EGFP
 
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1+ mentions
RRID:Addgene_16545 p73 Homo sapiens Ampicillin PMID:10588737 Tet-responsive p73 expression construct. Backbone Size:5210; Vector Backbone:pBI-MCS-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:29 1
pSico_U6-Pan-PGC1a sgRNA
 
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RRID:Addgene_165426 gRNA against human Total PGC-1a variants Homo sapiens Ampicillin PMID:33626346 Backbone Size:9580; Vector Backbone:pSico; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:09:28 0
pGST-PPARalpha
 
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RRID:Addgene_16550 PPAR alpha Homo sapiens Ampicillin PMID:10555149 Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX-2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin N-terminal DNA-binding domain: aa 1-249 2026-08-15 01:09:29 0
pSIN-TRE-H2BeGFP-rtTA2
 
Resource Report
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1+ mentions
RRID:Addgene_165494 Human histone H2B Homo sapiens Ampicillin PMID:29944140 ABOUT BACKBONE: The lentiviral backbone was originally designed and constructed by Barde I et al. (Barde I et al. Mol Ther. 2006. 13(2): 382-90. PMID: 16275162). ABOUT TEST DIGESTION: Since Xba I restriction site is lost during cloning, we recommend checking the plasmid using the enzymes: Xho I and Nhe I. You should get two fragments around 1600 bp (insert+vector) and 7500 bp (vector). Backbone Marker:-; Backbone Size:8023; Vector Backbone:pRRL-cPPT-hPGKTMPrtTA-WPRE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin - 2026-08-15 01:09:29 1
dCas9-MSK1
 
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RRID:Addgene_165601 ribosomal protein S6 kinase A5 Homo sapiens Ampicillin PMID:33563994 Vector Backbone:LentiCRISPR v2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:09:31 0
pIVEX2.3 TEM-5
 
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RRID:Addgene_16561 TEM5 Homo sapiens Ampicillin PMID:11559528 Backbone Marker:Boehringer; Backbone Size:3553; Vector Backbone:pIVEX2.3-MCS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Extracellular region 2026-08-15 01:09:31 0
pGEX TEM-1
 
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RRID:Addgene_16560 TEM1 Homo sapiens Ampicillin PMID:11559528 Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX-2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Extracellular region 2026-08-15 01:09:30 0
pGL3-OF (mutant TCF-4 sites)
 
Resource Report
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10+ mentions
RRID:Addgene_16559 TCF-4 binding sites Homo sapiens Ampicillin PMID:10749138 TCF-4 mutant binding sequence - CCTTTGGC Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin 3 copies of mutant Tcf-4 binding sites 2026-08-15 01:09:30 11
MadMyc-HA
 
Resource Report
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1+ mentions
RRID:Addgene_16557 MADMYC Homo sapiens Ampicillin PMID:10688915 MADMYC: the amino terminal transactivation domain of c-Myc (aa 1-263) was removed and replaced with the amino terminal transcriptional repression domain (aa 1-38, the Sin3-interaction region) of Mad. Backbone Size:0; Vector Backbone:n/a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Dominant negative mutant 2026-08-15 01:09:30 2

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