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Species: Homo sapiens
Genetic Insert: CDX2
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:7462; Vector Backbone:pHRCMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10490837
Comments: RKO (colorectal cancer cell line) mutation: 4 base-pair deletion (AGGCAGG to AGG) within the sequence corresponding to helix 3 of the CDX2 homeodomain.
Proper citation: RRID:Addgene_16554 Copy
Species: Homo sapiens
Genetic Insert: CDX2
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:7462; Vector Backbone:pHRCMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10490837
Proper citation: RRID:Addgene_16553 Copy
Species: Homo sapiens
Genetic Insert: 14-3-3 sigma
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:7462; Vector Backbone:pHRCMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9659898
Proper citation: RRID:Addgene_16552 Copy
Species: Homo sapiens
Genetic Insert: PTTG1
Vector Backbone Description: Backbone Size:3939; Vector Backbone:pFredB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11371342
Comments: 5 kb 3' targeting element, should be linearized with SalI prior to transfection.
Proper citation: RRID:Addgene_16585 Copy
Species: Homo sapiens
Genetic Insert: c-MYC binding sites
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:4867; Vector Backbone:pBV-Luc; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10688915
Comments: Different combinations of oligonucleotide pairs (A+B, A+D, C+B, C+D) were annealed and converted to double-stranded fragments through one PCR cycle. These promoter fragments were subcloned into pBV-luc. Further polymerase-derived mutants were identified while sequencing the reporter constructs.
Proper citation: RRID:Addgene_16566 Copy
Species: Homo sapiens
Genetic Insert: c-MYC binding sites
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:4867; Vector Backbone:pBV-Luc; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10688915
Comments: To generate reporter constructs, the following oligonucleotides were used:
5'-CCGGTACCGG GTTGTGGCAG CCAGTCACCT GCCCGCCGCG TAGCCACACC TCTGCTCCTC AGAGCAATGT CAAGCGGTCA CCTGTGATAG CAACAGATCA CCTGGCTGCC ATCGCCCCTC-3' [Oligo C , for mutant MBS1-3];
5'-ATGAATTCCG GACGTTCTGG GCAGGTGACC GCCACCCATG CGCTGAGGGG CGGACAGGAG GTGCTTCGAC TGGGAGGAGG GCGAAGAGTG TAAGGGGGCG GAGGGGCGAT GGCAGCCAGG-3' [Oligo D, for mutant MBS4].
Different combinations of oligonucleotide pairs (A+B, A+D, C+B, C+D) were annealed and converted to double-stranded fragments through one PCR cycle. These promoter fragments were subcloned into pBV-luc. Further polymerase-derived mutants were identified while sequencing the reporter constructs.
Proper citation: RRID:Addgene_16565 Copy
Species: Homo sapiens
Genetic Insert: c-MYC binding sites
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:4867; Vector Backbone:pBV-Luc; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10688915
Comments: To generate reporter constructs, the following oligonucleotides were used:
5'-CCGGTACCGG GTTGTGGCAG CCAGTCACGT GCCCGCCGCG TAGCCACACC TCTGCTCCTC AGAGCAATGT CAAGCGGTCA CGTGTGATAG CAACAGATCA CGTGGCTGCC ATCGCCCCTC-3' [Oligo A, for wild-type c-MYC binding sites (MBS)1-3]; 5'-ATGAATTCCG GACGTTCTGG GCACGTGACC GCCACCCATG CGCTGAGGGG CGGACAGGAG GTGCTTCGAC TGGGAGGAGG GCGAAGAGTG TAAGGGGGCG GAGGGGCGAT GGCAGCC-3' [Oligo B, for wild-type MBS4].
Different combinations of oligonucleotide pairs (A+B, A+D, C+B, C+D) were annealed and converted to double-stranded fragments through one PCR cycle. These promoter fragments were subcloned into pBV-luc. Further polymerase-derived mutants were identified while sequencing the reporter constructs.
Proper citation: RRID:Addgene_16564 Copy
Species: Homo sapiens
Genetic Insert: cAPC
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:0; Vector Backbone:pShuttle / pAdTrack ?; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10749138
Comments: The cAPC containing amino acids 958-2075 (nucleotides 2890–6240) was isolated from pCMV-APC by BglII digestion. This fragment was subcloned into the pEGFP-C1 (Clontech). The cassette containing the EGFP-tagged cAPC was further subcloned into the shuttle vector (pShuttle) using Apal I and Mlu I sites.
Proper citation: RRID:Addgene_16576 Copy
Species: Homo sapiens
Genetic Insert: BAX knockout
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript II SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11062132
Comments: This targeting construct consists of two homologous arms and a hygromycin-resistant gene.
Proper citation: RRID:Addgene_16575 Copy
Species: Homo sapiens
Genetic Insert: CDK4
Vector Backbone Description: Backbone Size:0; Vector Backbone:G1BgSVNA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10688915
Comments: The CDK4 ORF was generated by PCR using the expressed sequence tag W77860 as a template and the primers 5'-GCGGATCCGC GGCCGCCTTC CACCATGGCT ACCTCTCGAT CTGAGC-3' and 5'-CGGTCGACTC ACTCCGGATT ACCTTCATC-3'. The resulting product was digested with the enzymes NotI and SalI and inserted into the respective sites of the vector G1BgSVNA (a retroviral vector encoding a hygromycin resistance gene and beta-galactosidase).
Proper citation: RRID:Addgene_16573 Copy
Species: Homo sapiens
Genetic Insert: CDK4 promoter
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4360; Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10688915
Proper citation: RRID:Addgene_16571 Copy
Species: Homo sapiens
Genetic Insert: PUMA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10400; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11463391
Comments: Contains a 15-amino acid deletion encompassing the BH3 domain (AA 136–145).
Proper citation: RRID:Addgene_16589 Copy
Species: Homo sapiens
Genetic Insert: BAX alpha
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10400; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11463391
Proper citation: RRID:Addgene_16587 Copy
Species: Homo sapiens
Genetic Insert: c-myc promoter (4xTBE1 wt)
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:4867; Vector Backbone:pBV-Luc; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9727977
Comments: Tcf-4 binding elements (TBEs). To construct the four tandem repeats of TBE1, we dimerized oligonucleotide cassettes containing two copies of the site and cloned the products into pBV-Luc (5'-CTAGCGCACCTTTGATTTCTGCACCTTTGATTTCTG-3' and 5'-CTAGCAGAAATCAAAGGTGCAGAAATCAAAGGTGCG-3')
Proper citation: RRID:Addgene_16597 Copy
Species: Homo sapiens
Genetic Insert: p53 binding sites
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:4867; Vector Backbone:pBV-Luc; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11463391
Comments: The following oligo pairs containing two copies of wild-type p53 binding sites were used: 5'-CTAGGCTGCA AGTCCTGACT TGTCCACACT CTGCAAGTCC TGACTTGTCC-3' and 5'-CTAGGGACAA GTCAGGACTT GCAGAGTGTG GACAAGTCAG GACTTGCAGC-3'. The annealed oligonucleotide pairs were concatamerized and cloned into the NheI site of pBVLuc.
Proper citation: RRID:Addgene_16593 Copy
Species: Homo sapiens
Genetic Insert: PUMA promotor fragment
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:4867; Vector Backbone:pBV-Luc; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11463391
Comments: Deletion of 5' terminal 300 bp from Frag1. Does not contain consensus p53 binding sites BS1 and BS2.
Proper citation: RRID:Addgene_16592 Copy
Species: Homo sapiens
Genetic Insert: HuT1head1-405(T144E,T150E)
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific, Carlsbad, CA; Vector Backbone:pTrcHis C; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33046605
Proper citation: RRID:Addgene_166130 Copy
Species: Homo sapiens
Genetic Insert: T1head1-405(del37/GAG)
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific, Carlsbad, CA; Vector Backbone:pTrcHis C; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33046605
Proper citation: RRID:Addgene_166129 Copy
Species: Homo sapiens
Genetic Insert: T1head1-405(del30)
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific, Carlsbad, CA; Vector Backbone:pTrcHis C; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33046605
Proper citation: RRID:Addgene_166128 Copy
Species: Homo sapiens
Genetic Insert: Tln1Head (1-405)
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific, Carlsbad, CA; Vector Backbone:pTrcHis C; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33046605
Proper citation: RRID:Addgene_166127 Copy
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