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Species: Homo sapiens
Genetic Insert: CENP-O, IMAGE clone 3942844
Vector Backbone Description: Vector Backbone:pJAG98 (pBABEblast); Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_63068 Copy
Species: Homo sapiens
Genetic Insert: CENP-P, IMAGE clone 5554572
Vector Backbone Description: Vector Backbone:pJAG98 (pBABEblast); Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_63069 Copy
Vector Backbone Description: Backbone Size:4400; Vector Backbone:pRS403; Vector Types:Yeast Expression, Worm Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25756291
Proper citation: RRID:Addgene_63193 Copy
Vector Backbone Description: Backbone Size:4200; Vector Backbone:pRS404; Vector Types:Bacterial Expression, Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25756291
Proper citation: RRID:Addgene_63194 Copy
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pRS424; Vector Types:Bacterial Expression, Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25756291
Proper citation: RRID:Addgene_63190 Copy
Species: Homo sapiens
Genetic Insert: hspb10
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20843828
Proper citation: RRID:Addgene_63111 Copy
Vector Backbone Description: Backbone Size:4400; Vector Backbone:pRS406; Vector Types:Bacterial Expression, Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25756291
Proper citation: RRID:Addgene_63196 Copy
Species: Homo sapiens
Genetic Insert: hspb9
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20843828
Proper citation: RRID:Addgene_63110 Copy
Species: Homo sapiens
Genetic Insert: expanded CGG repeats (99 repeats) within the 5'UTR of FMR1
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Should be amplified in STBL3 bacteria and grown at Room temperature to keep the repeats stable.
This construct allows translation of CGG repeats into 99 glycine due to a near cognate ATG codon upstream of the repeat in the 5'UTR of FMR1 (an ACG codon that initiates weakly translation).
This plasmid should be verified via restriction analysis. Multiple colonies may need to be screened to isolate the correct construct.
Proper citation: RRID:Addgene_63089 Copy
Species: Other
Genetic Insert: Mm-cpn c7_87/199_HLL
Vector Backbone Description: Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24270642
Proper citation: RRID:Addgene_63122 Copy
Species: Synthetic
Genetic Insert: CCUG 1200x
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: Should be amplified in STBL3 bacteria and grown at Room temperature to keep the repeats stable.
This plasmid should be verified via restriction analysis. Multiple colonies may need to be screened to isolate the correct construct.
Proper citation: RRID:Addgene_63088 Copy
Species: Homo sapiens
Genetic Insert: hspb2
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20843828
Proper citation: RRID:Addgene_63093 Copy
Species: Homo sapiens
Genetic Insert: hspb3
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20843828
Proper citation: RRID:Addgene_63094 Copy
Species: Homo sapiens
Genetic Insert: HOIL-1L
Vector Backbone Description: Backbone Marker:GE Healthcare; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22863777
Proper citation: RRID:Addgene_63135 Copy
Species: Homo sapiens
Genetic Insert: hspb5
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20843828
Proper citation: RRID:Addgene_63096 Copy
Species: Homo sapiens
Genetic Insert: hspb7
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20843828
Proper citation: RRID:Addgene_63098 Copy
Vector Backbone Description: Vector Backbone:pXR; Vector Types:Bacterial Expression, Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25736914
Proper citation: RRID:Addgene_63144 Copy
Vector Backbone Description: Vector Backbone:pXR; Vector Types:Bacterial Expression, Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25736914
Proper citation: RRID:Addgene_63145 Copy
Vector Backbone Description: Vector Backbone:pXR; Vector Types:Bacterial Expression, Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25736914
Proper citation: RRID:Addgene_63146 Copy
Species: Halorubrum sp. TP009
Genetic Insert: ArchT-GFP
Vector Backbone Description: Backbone Marker:original from Stratagene; Backbone Size:4707; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21811444
Comments: The plasmid is sequenced completely by the depositing lab except for parts of both ITRs. Multiple digestions were done to verify the vector structure. The construct and the virus were both tested in vitro.
For additional information on Arch, please see Chow et al., Nature. 2010 Jan 7. 463(7277):98-102. PubMed ID: 20054397
Proper citation: RRID:Addgene_63141 Copy
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