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Species: Mus musculus
Genetic Insert: Protein arginine methyltransferase 7
Vector Backbone Description: Backbone Marker:System Biosciences; Backbone Size:7200; Vector Backbone:pCDH-EF1-IRES-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27625395
Proper citation: RRID:Addgene_87104 Copy
Species: Mus musculus
Genetic Insert: Stard13
Vector Backbone Description: Vector Backbone:pCS2++; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_87128 Copy
Species: Mus musculus
Genetic Insert: Prox1
Vector Backbone Description: Vector Backbone:TOPOII; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: For antisense probe: digest with HindIII and use T7 polymerase.
Proper citation: RRID:Addgene_87129 Copy
Species: Mus musculus
Genetic Insert: TRIM30A
Vector Backbone Description: Backbone Size:5427; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26468522
Proper citation: RRID:Addgene_87081 Copy
Species: Mus musculus
Genetic Insert: TRIM30D
Vector Backbone Description: Backbone Size:5427; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26468522
Proper citation: RRID:Addgene_87084 Copy
Species: Mus musculus
Genetic Insert: Stat4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pRc/CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7545930
Comments: Diagnostic restriction digest with BamHI (3.1kb, 2.0kb, 1.9kb, and 0.9kb).
Proper citation: RRID:Addgene_8711 Copy
Species: Mus musculus
Genetic Insert: hdac8
Vector Backbone Description: Backbone Size:4113; Vector Backbone:pCS2++; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_87130 Copy
Species: Mus musculus
Genetic Insert: TZAP
Vector Backbone Description: Vector Backbone:pPHAGE2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28082411
Proper citation: RRID:Addgene_87188 Copy
Species: Mus musculus
Genetic Insert: ZFP57
Vector Backbone Description: Vector Backbone:NEB DHFR control plasmid; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29159284
Comments: This construct has the DNA binding domain of mouse ZFP57 including two separate groups of zinc fingers and the linker in between. In our published work about methylation sensitivity assay, only the truncated first group of Zinc finger (F1-F3) was used for Methyl-Spec-seq assay.
Proper citation: RRID:Addgene_87224 Copy
Species: Mus musculus
Genetic Insert: Stat3-C
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pRc/CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10458605
Comments: Residues changed to Cys renders the molecule constitutively active. Diagnostic Restriction Digest with BamHI (3.2kb, 2.8kb, and 2.0kb). Due to a stop codon error made when tagging the Stat3 clone with FLAG (DYKDDDDK) additional residues precede (SR) and follow the tag (GPYSIVSPKC STOP). The ApaI cloning site is GGG CCC, which encodes the G and P residues coming after the FLAG tag. Therefore, if the insert is removed with a NotI and ApaI digestion, it will not have a stop codon included.
* E16K mutation found during QC
Proper citation: RRID:Addgene_8722 Copy
Species: Mus musculus
Genetic Insert: CXADR
Vector Backbone Description: Backbone Marker:Sigma; Vector Backbone:pFlag-CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_87239 Copy
Species: Mus musculus
Genetic Insert: CXADR
Vector Backbone Description: Backbone Marker:Sigma; Vector Backbone:pFlag-CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_87236 Copy
Species: Mus musculus
Genetic Insert: CXADR
Vector Backbone Description: Backbone Marker:Sigma; Vector Backbone:pFlag-CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_87237 Copy
Species: Mus musculus
Genetic Insert: TBK1
Vector Backbone Description: Backbone Size:6890; Vector Backbone:pEGFPC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29445180
Proper citation: RRID:Addgene_87443 Copy
Species: Mus musculus
Genetic Insert: Bcl-2
Vector Backbone Description: Backbone Size:7200; Vector Backbone:pSFFV-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8358790
Proper citation: RRID:Addgene_8750 Copy
Species: Mus musculus
Genetic Insert: Bcl-XL (Exon 1)
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript KS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7650488
Proper citation: RRID:Addgene_8755 Copy
Species: Mus musculus
Genetic Insert: Bax
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript II KS(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7569956
Comments: for in situ probes: antisense - cut with PstI and use T7 polymerase. sense - cut with SalI and use T3 polymerase.
Proper citation: RRID:Addgene_8754 Copy
Species: Mus musculus
Genetic Insert: BAD
Vector Backbone Description: Backbone Size:7200; Vector Backbone:pSFFV-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7834748
Comments: The expression plasmid pSFFVHA-Bad was constructed by using PCR primers, which incorporated the 9 codons of the HA epitope onto the 5' end of Bad, and it was ligated into pSFFV vector at the EcoRI site.
Proper citation: RRID:Addgene_8753 Copy
Species: Mus musculus
Genetic Insert: Bid p22 ORF cDNA FL
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8918887
Proper citation: RRID:Addgene_8767 Copy
Species: Mus musculus
Genetic Insert: Hox11 L1
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:3820; Vector Backbone:pSHLox; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9176491
Comments: Used for internal probe: 1.9 kb EcoRI-HindIII fragment.
Proper citation: RRID:Addgene_8761 Copy
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