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Vector Backbone Description: Vector Backbone:pCWX; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: This plasmid allows for 3-plasmid Gateway cloning of a gene of interest with a promoter of choice. GOI is in pENTRL1L2 and promoter is in pENTRL4L1R
Proper citation: RRID:Addgene_114319 Copy
Species: Homo sapiens
Genetic Insert: Ras
Vector Backbone Description: Vector Backbone:pCLXEBR65; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: Due to the role of the transgene in cell proliferation, lentivectors produced with human-tropic envelopes must be produced in BSL2+/BSL3 conditions. Subcloned from Addgene Plasmid #31200. pTF is an optimized TET-promoter, described in HGT 2011, Giry et al.
Proper citation: RRID:Addgene_114318 Copy
Species: Mus musculus
Genetic Insert: mBmi1
Vector Backbone Description: Vector Backbone:pCLXEBR65; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23097121
Comments: Due to the role of the transgene in cell proliferation, lentivectors produced with human-tropic envelopes must be produced in BSL2+/BSL3 conditions. pTF is an optimized TET-promoter, described in HGT 2011, Giry et al.
Proper citation: RRID:Addgene_114317 Copy
Species: Homo sapiens
Genetic Insert: hTERT
Vector Backbone Description: Vector Backbone:pCWX-PGK-BSD; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23097121
Comments: Due to the role of the transgene in cell proliferation, lentivectors produced with human-tropic envelopes must be produced in BSL2+/BSL3 conditions.
Proper citation: RRID:Addgene_114316 Copy
Species: Homo sapiens
Genetic Insert: TFDP2
Vector Backbone Description: Vector Backbone:pWPI-DEST; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23907129
Comments: Due to the role of the transgene in cell proliferation, lentivectors produced with human-tropic envelopes must be produced in BSL2+/BSL3 conditions.
Proper citation: RRID:Addgene_114300 Copy
Species: Homo sapiens
Genetic Insert: Ngn3
Vector Backbone Description: Vector Backbone:pCWXPG; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21761975
Proper citation: RRID:Addgene_114303 Copy
Species: Homo sapiens
Genetic Insert: MafA
Vector Backbone Description: Vector Backbone:pCWXPG; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21761975
Proper citation: RRID:Addgene_114302 Copy
Species: Homo sapiens
Genetic Insert: Ngn3
Vector Backbone Description: Vector Backbone:pCLXEBR65; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21761975
Comments: pTF is an optimized TET-promoter, described in HGT 2011, Giry et al.
Proper citation: RRID:Addgene_114307 Copy
Species: Homo sapiens
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:pCLX; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_114309 Copy
Species: Homo sapiens
Genetic Insert: gp78c
Vector Backbone Description: Backbone Marker:Amersham/GE; Backbone Size:5000; Vector Backbone:pGEX-4T2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11724934
Proper citation: RRID:Addgene_11429 Copy
Species: Mus musculus
Genetic Insert: anti-VGlut3 (Rattus norvegicus) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30667360
Comments: RRID: AB_2750780. Isotype: IgG2a. Additional species: mouse, rat.
A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_114494 Copy
Species: Mus musculus
Genetic Insert: anti-NGL-3/LRRC4B (Rattus norvegicus) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30667360
Comments: RRID: AB_2750799. Isotype: IgG2a. Additional species: rat, mouse.
A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_114493 Copy
Species: Homo sapiens
Genetic Insert: MYL2 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30956114
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 in a two-step editing process to add a mEGFP tag to the C-terminus of human MYL2 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. After initial HDR, stable expression of CAGGS-driven mCherry will indicate cells with integrated donor sequence. This selection cassette is designed to be removed with subsequent CRISPR/Cas9-mediated excision as previously described (https://doi.org/10.1101/342881). A linker of 3-21 amino acids will be produced after excision. To enrich for edited cells, see our protocol for fluorsecence-assisted cell sorting and subcloning of hiPSCs (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209).
For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/.
Please visit https://www.biorxiv.org/content/early/2018/06/09/342881 for the bioRxiv preprint.
Proper citation: RRID:Addgene_114414 Copy
Species: Homo sapiens
Genetic Insert: TNNI1 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30956114
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 in a two-step editing process to add a mEGFP tag to the C-terminus of human TNNI1 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. After initial HDR, stable expression of hPGK-driven mCherry will indicate cells with integrated donor sequence. This selection cassette is designed to be removed with subsequent CRISPR/Cas9-mediated excision as previously described (https://doi.org/10.1101/342881). A linker of 3-21 amino acids will be produced after excision. To enrich for edited cells, see our protocol for fluorsecence-assisted cell sorting and subcloning of hiPSCs (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209).
For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/.
Please visit https://www.biorxiv.org/content/early/2018/06/09/342881 for the bioRxiv preprint.
Proper citation: RRID:Addgene_114411 Copy
Species: Synthetic
Genetic Insert: iLID domain
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30182785
Proper citation: RRID:Addgene_114418 Copy
Species: Synthetic
Genetic Insert: iLID domain
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30182785
Proper citation: RRID:Addgene_114417 Copy
Species: Synthetic
Genetic Insert: iLID domain
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30182785
Proper citation: RRID:Addgene_114415 Copy
Species: Homo sapiens
Genetic Insert: MLL-AF4 fusion gene
Vector Backbone Description: Backbone Marker:Rudolf Jaenisch, Addgene Plasmid #22075; Backbone Size:6602; Vector Backbone:AAVS1 SA-2A-puro-pA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29386396
Proper citation: RRID:Addgene_114380 Copy
Species: Mus musculus
Genetic Insert: anti-Synaptotagmin-12 (Mus musculus) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30667360
Comments: RRID: AB_2750763. Isotype: IgG2a. Additional species: mouse, rat.
A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_114482 Copy
Species: Mus musculus
Genetic Insert: anti-SUR2B (Rattus norvegicus) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30667360
Comments: RRID: AB_2750775. Isotype: IgG2a. Additional species: rat, mouse, human.
A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_114480 Copy
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